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Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins

BACKGROUND: Exosomes and other types of extracellular vesicles present an important component of circulating plasma. Exosomes released by endothelial and blood cells account for majority of plasma exosomal population; exosomes secreted by other cells might cross tissue-plasma barrier and reach circu...

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Autores principales: Shtam, Tatiana, Naryzhny, Stanislav, Kopylov, Arthur, Petrenko, Elena, Samsonov, Roman, Kamyshinsky, Roman, Zabrodskaya, Yana, Nikitin, Daniil, Sorokin, Maxim, Buzdin, Anton, Malek, Anastasia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elmer Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155850/
https://www.ncbi.nlm.nih.gov/pubmed/32300430
http://dx.doi.org/10.14740/jh412w
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author Shtam, Tatiana
Naryzhny, Stanislav
Kopylov, Arthur
Petrenko, Elena
Samsonov, Roman
Kamyshinsky, Roman
Zabrodskaya, Yana
Nikitin, Daniil
Sorokin, Maxim
Buzdin, Anton
Malek, Anastasia
author_facet Shtam, Tatiana
Naryzhny, Stanislav
Kopylov, Arthur
Petrenko, Elena
Samsonov, Roman
Kamyshinsky, Roman
Zabrodskaya, Yana
Nikitin, Daniil
Sorokin, Maxim
Buzdin, Anton
Malek, Anastasia
author_sort Shtam, Tatiana
collection PubMed
description BACKGROUND: Exosomes and other types of extracellular vesicles present an important component of circulating plasma. Exosomes released by endothelial and blood cells account for majority of plasma exosomal population; exosomes secreted by other cells might cross tissue-plasma barrier and reach circulating plasma as well. Definitely, exosomes of different cellular origins are different by content and function. However, exosomal surface membrane interacts with plasma components. This interaction may alter composition of exosomal surface and hence, provide these vesicles with new functional properties. This study was aimed to estimate composition and possible functional role of proteins attached on the surface of plasma exosomes. METHODS: Here, extracellular vesicles from human plasma were isolated by ultracentrifugation and treated by trypsin. Trypsinized and native exosomes were analyzed by nanoparticle tracking analysis, Western blotting and quantitative high-resolution mass spectrometry. RESULTS: Surface-attached proteins were removed from exosomes isolated from plasma of healthy donors by incubation with serine protease (trypsin). Treatment did not impact exosomes integrity while slightly reduced hydrodynamic radius. Mass spectrometry revealed 259 exosomal proteins; among them 79 proteins were completely removed and more than half of the proteins were partially removed by trypsinization. Gene ontology functional annotation revealed mostly extracellular locations of proteins cleaved from a surface of the plasma exosomes. Moreover, proteins cleaved from the exosome surface are supposed to be implicated into integrin-linked kinase (ILK), focal adhesion kinase (FAK) and other pathways connecting cell surface with intracellular signaling cascades. CONCLUSION: Taken together, our results demonstrate that a surface of circulating exosomes is decorated by plasma proteins, and these proteins can mask tissue-specific characteristic of the exosomal surface membrane and provide exosomes with new and uniform properties.
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spelling pubmed-71558502020-04-16 Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins Shtam, Tatiana Naryzhny, Stanislav Kopylov, Arthur Petrenko, Elena Samsonov, Roman Kamyshinsky, Roman Zabrodskaya, Yana Nikitin, Daniil Sorokin, Maxim Buzdin, Anton Malek, Anastasia J Hematol Short Communication BACKGROUND: Exosomes and other types of extracellular vesicles present an important component of circulating plasma. Exosomes released by endothelial and blood cells account for majority of plasma exosomal population; exosomes secreted by other cells might cross tissue-plasma barrier and reach circulating plasma as well. Definitely, exosomes of different cellular origins are different by content and function. However, exosomal surface membrane interacts with plasma components. This interaction may alter composition of exosomal surface and hence, provide these vesicles with new functional properties. This study was aimed to estimate composition and possible functional role of proteins attached on the surface of plasma exosomes. METHODS: Here, extracellular vesicles from human plasma were isolated by ultracentrifugation and treated by trypsin. Trypsinized and native exosomes were analyzed by nanoparticle tracking analysis, Western blotting and quantitative high-resolution mass spectrometry. RESULTS: Surface-attached proteins were removed from exosomes isolated from plasma of healthy donors by incubation with serine protease (trypsin). Treatment did not impact exosomes integrity while slightly reduced hydrodynamic radius. Mass spectrometry revealed 259 exosomal proteins; among them 79 proteins were completely removed and more than half of the proteins were partially removed by trypsinization. Gene ontology functional annotation revealed mostly extracellular locations of proteins cleaved from a surface of the plasma exosomes. Moreover, proteins cleaved from the exosome surface are supposed to be implicated into integrin-linked kinase (ILK), focal adhesion kinase (FAK) and other pathways connecting cell surface with intracellular signaling cascades. CONCLUSION: Taken together, our results demonstrate that a surface of circulating exosomes is decorated by plasma proteins, and these proteins can mask tissue-specific characteristic of the exosomal surface membrane and provide exosomes with new and uniform properties. Elmer Press 2018-12 2018-11-22 /pmc/articles/PMC7155850/ /pubmed/32300430 http://dx.doi.org/10.14740/jh412w Text en Copyright 2018, Shtam et al. http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Non-Commercial 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Shtam, Tatiana
Naryzhny, Stanislav
Kopylov, Arthur
Petrenko, Elena
Samsonov, Roman
Kamyshinsky, Roman
Zabrodskaya, Yana
Nikitin, Daniil
Sorokin, Maxim
Buzdin, Anton
Malek, Anastasia
Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins
title Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins
title_full Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins
title_fullStr Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins
title_full_unstemmed Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins
title_short Functional Properties of Circulating Exosomes Mediated by Surface-Attached Plasma Proteins
title_sort functional properties of circulating exosomes mediated by surface-attached plasma proteins
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155850/
https://www.ncbi.nlm.nih.gov/pubmed/32300430
http://dx.doi.org/10.14740/jh412w
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