Evaluation of Blood Collection From the Proximal Side of a Fluid Infusion Site

BACKGROUND: We aimed to determine if blood data are unaffected by transfusion method when the blood is sampled from the proximal side of the infusion site under temporary suspension of transfusion. METHODS: In five 30-week-old Japanese white male rabbits, fluid infusion routes were secured via left auricular veins with a disposable plastic indwelling cannula (24-G needle). Solita T3 (22 mL/h) was administered to each animal using a syringe pump. Ten minutes after starting infusion, 2 mL blood was sampled from a distance of 2 cm on the central side of the infusion site with ongoing fluid infusion, from the opposite side (the right auricular vein), and from the central side of the infusion site with suspended fluid infusion. We cross-verified and compared results of 41 biochemical and blood cell examination items for samples collected from the central side with ongoing fluid infusion, from the central side with suspended fluid infusion, and from the opposite sides by comparison with Tukey’s test. RESULTS: A significant difference was noted in tested items between blood samples collected from the proximal side with ongoing fluid infusion and those collected from the proximal side and from the contralateral side with suspended fluid infusion. Conversely, for all test items, no significant difference was noted in the test item data between blood samples collected from the proximal side with suspended fluid infusion and those collected from the contralateral side. CONCLUSIONS: We successfully verified and demonstrated that blood samples collected from the proximal side of the infusion site remain unaffected by fluid infusion when drawn under the conditions of suspended fluid infusion.