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Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure

The Polycomb group (PcG) proteins are chromatin factors underlying the process of transcriptional memory to preserve developmental decisions and keep cellular identities. However, not only developmental signals need to be memorized and thus maintained during the life of an organism. For host protect...

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Autores principales: Geigges, Marco, Arekatla, Geethika, Paro, Renato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156044/
https://www.ncbi.nlm.nih.gov/pubmed/32287290
http://dx.doi.org/10.1371/journal.pone.0231498
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author Geigges, Marco
Arekatla, Geethika
Paro, Renato
author_facet Geigges, Marco
Arekatla, Geethika
Paro, Renato
author_sort Geigges, Marco
collection PubMed
description The Polycomb group (PcG) proteins are chromatin factors underlying the process of transcriptional memory to preserve developmental decisions and keep cellular identities. However, not only developmental signals need to be memorized and thus maintained during the life of an organism. For host protection against pathogens, also a memory of previous exposures to an immunogenic stimulus is crucial to mount a more protective immune response upon re-exposure. The antigen-specific adaptive immunity in vertebrates is an example of such a memory to previous immunogenic stimulation. Recently, adaptive characteristics were also attributed to innate immunity, which was classically seen to lack memory. However, the mechanistic details of an adaptive innate immune response are yet to be fully understood and chromatin-based epigenetic mechanisms seem to play an important role in this phenomenon. Possibly, PcG proteins can contribute to such an epigenetic innate immune memory. In this study, we analyzed whether the PcG system can mediate a transcriptional memory of exposure to lipopolysaccharides (LPS). To this end, various forms of LPS pre-treatment were applied to reporter cells and expression kinetics of PcG target genes were analyzed after a second LPS exposure. Neither single nor multiple LPS pre-treatment affected the induction of endogenous LPS-responsive transcripts upon re-exposure. Altogether, our extensive analyses did not provide any evidence for a PcG system-mediated memory of LPS stimulation.
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spelling pubmed-71560442020-04-16 Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure Geigges, Marco Arekatla, Geethika Paro, Renato PLoS One Research Article The Polycomb group (PcG) proteins are chromatin factors underlying the process of transcriptional memory to preserve developmental decisions and keep cellular identities. However, not only developmental signals need to be memorized and thus maintained during the life of an organism. For host protection against pathogens, also a memory of previous exposures to an immunogenic stimulus is crucial to mount a more protective immune response upon re-exposure. The antigen-specific adaptive immunity in vertebrates is an example of such a memory to previous immunogenic stimulation. Recently, adaptive characteristics were also attributed to innate immunity, which was classically seen to lack memory. However, the mechanistic details of an adaptive innate immune response are yet to be fully understood and chromatin-based epigenetic mechanisms seem to play an important role in this phenomenon. Possibly, PcG proteins can contribute to such an epigenetic innate immune memory. In this study, we analyzed whether the PcG system can mediate a transcriptional memory of exposure to lipopolysaccharides (LPS). To this end, various forms of LPS pre-treatment were applied to reporter cells and expression kinetics of PcG target genes were analyzed after a second LPS exposure. Neither single nor multiple LPS pre-treatment affected the induction of endogenous LPS-responsive transcripts upon re-exposure. Altogether, our extensive analyses did not provide any evidence for a PcG system-mediated memory of LPS stimulation. Public Library of Science 2020-04-14 /pmc/articles/PMC7156044/ /pubmed/32287290 http://dx.doi.org/10.1371/journal.pone.0231498 Text en © 2020 Geigges et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Geigges, Marco
Arekatla, Geethika
Paro, Renato
Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure
title Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure
title_full Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure
title_fullStr Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure
title_full_unstemmed Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure
title_short Priming exposures to lipopolysaccharides do not affect the induction of Polycomb target genes upon re-exposure
title_sort priming exposures to lipopolysaccharides do not affect the induction of polycomb target genes upon re-exposure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156044/
https://www.ncbi.nlm.nih.gov/pubmed/32287290
http://dx.doi.org/10.1371/journal.pone.0231498
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