Cargando…
Lipidomic study of cell lines reveals differences between breast cancer subtypes
Breast cancer (BC) is the most prevalent type of cancer in women in western countries. BC mortality has not declined despite early detection by screening, indicating the need for better informed treatment decisions. Therefore, a novel noninvasive diagnostic tool for BC would give the opportunity of...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156077/ https://www.ncbi.nlm.nih.gov/pubmed/32287294 http://dx.doi.org/10.1371/journal.pone.0231289 |
_version_ | 1783522146532720640 |
---|---|
author | Eiriksson, Finnur Freyr Nøhr, Martha Kampp Costa, Margarida Bödvarsdottir, Sigridur Klara Ögmundsdottir, Helga Margret Thorsteinsdottir, Margret |
author_facet | Eiriksson, Finnur Freyr Nøhr, Martha Kampp Costa, Margarida Bödvarsdottir, Sigridur Klara Ögmundsdottir, Helga Margret Thorsteinsdottir, Margret |
author_sort | Eiriksson, Finnur Freyr |
collection | PubMed |
description | Breast cancer (BC) is the most prevalent type of cancer in women in western countries. BC mortality has not declined despite early detection by screening, indicating the need for better informed treatment decisions. Therefore, a novel noninvasive diagnostic tool for BC would give the opportunity of subtype-specific treatment and improved prospects for the patients. Heterogeneity of BC tumor subtypes is reflected in the expression levels of enzymes in lipid metabolism. The aim of the study was to investigate whether the subtype defined by the transcriptome is reflected in the lipidome of BC cell lines. A liquid chromatography mass spectrometry (LC-MS) platform was applied to analyze the lipidome of six cell lines derived from human BC cell lines representing different BC subtypes. We identified an increased abundance of triacylglycerols (TG) ≥ C-48 with moderate or multiple unsaturation in fatty acyl chains and down-regulated ether-phosphatidylethanolamines (PE) (C-34 to C-38) in cell lines representing estrogen receptor and progesterone receptor positive tumor subtypes. In a cell line representing HER2-overexpressing tumor subtype an elevated expression of TG (≤ C-46), phosphatidylcholines (PC) and PE containing short-chained (≤ C-16) saturated or monounsaturated fatty acids were observed. Increased abundance of PC ≥ C-40 was found in cell lines of triple negative BC subtype. In addition, differences were detected in lipidomes within these previously defined subtypes. We conclude that subtypes defined by the transcriptome are indeed reflected in differences in the lipidome and, furthermore, potentially biologically relevant differences may exist within these defined subtypes. |
format | Online Article Text |
id | pubmed-7156077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-71560772020-04-16 Lipidomic study of cell lines reveals differences between breast cancer subtypes Eiriksson, Finnur Freyr Nøhr, Martha Kampp Costa, Margarida Bödvarsdottir, Sigridur Klara Ögmundsdottir, Helga Margret Thorsteinsdottir, Margret PLoS One Research Article Breast cancer (BC) is the most prevalent type of cancer in women in western countries. BC mortality has not declined despite early detection by screening, indicating the need for better informed treatment decisions. Therefore, a novel noninvasive diagnostic tool for BC would give the opportunity of subtype-specific treatment and improved prospects for the patients. Heterogeneity of BC tumor subtypes is reflected in the expression levels of enzymes in lipid metabolism. The aim of the study was to investigate whether the subtype defined by the transcriptome is reflected in the lipidome of BC cell lines. A liquid chromatography mass spectrometry (LC-MS) platform was applied to analyze the lipidome of six cell lines derived from human BC cell lines representing different BC subtypes. We identified an increased abundance of triacylglycerols (TG) ≥ C-48 with moderate or multiple unsaturation in fatty acyl chains and down-regulated ether-phosphatidylethanolamines (PE) (C-34 to C-38) in cell lines representing estrogen receptor and progesterone receptor positive tumor subtypes. In a cell line representing HER2-overexpressing tumor subtype an elevated expression of TG (≤ C-46), phosphatidylcholines (PC) and PE containing short-chained (≤ C-16) saturated or monounsaturated fatty acids were observed. Increased abundance of PC ≥ C-40 was found in cell lines of triple negative BC subtype. In addition, differences were detected in lipidomes within these previously defined subtypes. We conclude that subtypes defined by the transcriptome are indeed reflected in differences in the lipidome and, furthermore, potentially biologically relevant differences may exist within these defined subtypes. Public Library of Science 2020-04-14 /pmc/articles/PMC7156077/ /pubmed/32287294 http://dx.doi.org/10.1371/journal.pone.0231289 Text en © 2020 Eiriksson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Eiriksson, Finnur Freyr Nøhr, Martha Kampp Costa, Margarida Bödvarsdottir, Sigridur Klara Ögmundsdottir, Helga Margret Thorsteinsdottir, Margret Lipidomic study of cell lines reveals differences between breast cancer subtypes |
title | Lipidomic study of cell lines reveals differences between breast cancer subtypes |
title_full | Lipidomic study of cell lines reveals differences between breast cancer subtypes |
title_fullStr | Lipidomic study of cell lines reveals differences between breast cancer subtypes |
title_full_unstemmed | Lipidomic study of cell lines reveals differences between breast cancer subtypes |
title_short | Lipidomic study of cell lines reveals differences between breast cancer subtypes |
title_sort | lipidomic study of cell lines reveals differences between breast cancer subtypes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156077/ https://www.ncbi.nlm.nih.gov/pubmed/32287294 http://dx.doi.org/10.1371/journal.pone.0231289 |
work_keys_str_mv | AT eirikssonfinnurfreyr lipidomicstudyofcelllinesrevealsdifferencesbetweenbreastcancersubtypes AT nøhrmarthakampp lipidomicstudyofcelllinesrevealsdifferencesbetweenbreastcancersubtypes AT costamargarida lipidomicstudyofcelllinesrevealsdifferencesbetweenbreastcancersubtypes AT bodvarsdottirsigridurklara lipidomicstudyofcelllinesrevealsdifferencesbetweenbreastcancersubtypes AT ogmundsdottirhelgamargret lipidomicstudyofcelllinesrevealsdifferencesbetweenbreastcancersubtypes AT thorsteinsdottirmargret lipidomicstudyofcelllinesrevealsdifferencesbetweenbreastcancersubtypes |