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Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters

INTRODUCTION: The genus Rhodnius in the subfamily Triatominae comprises 20 species, which can transmit Trypanosoma cruzi and Trypanosoma rangeli. Due to the development of molecular techniques, Triatominae species can now be characterized by mitochondrial and nuclear markers, making it possible to v...

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Autores principales: Falcone, Rossana, Ribeiro, Aline Rimoldi, de Oliveira, Jader, Mendonça, Vagner José, Graminha, Márcia, da Rosa, João Aristeu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156257/
https://www.ncbi.nlm.nih.gov/pubmed/32267457
http://dx.doi.org/10.1590/0037-8682-0503-2019
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author Falcone, Rossana
Ribeiro, Aline Rimoldi
de Oliveira, Jader
Mendonça, Vagner José
Graminha, Márcia
da Rosa, João Aristeu
author_facet Falcone, Rossana
Ribeiro, Aline Rimoldi
de Oliveira, Jader
Mendonça, Vagner José
Graminha, Márcia
da Rosa, João Aristeu
author_sort Falcone, Rossana
collection PubMed
description INTRODUCTION: The genus Rhodnius in the subfamily Triatominae comprises 20 species, which can transmit Trypanosoma cruzi and Trypanosoma rangeli. Due to the development of molecular techniques, Triatominae species can now be characterized by mitochondrial and nuclear markers, making it possible to verify and/or correct the existing data on these species. The results achieved in this study provide a more detailed and accurate differentiation of the Rhodnius species, helping the establishment of a more appropriate classification. METHODS: Data collection was performed by DNA analysis, morphological and morphometric studies to distinguish four populations of R. neglectus and four of R. prolixus. Phylogenetic data were compared to morphological and morphometric data. RESULTS: The analysis of Cytb fragments suggests that the four colonies designated to Rhodnius neglectus as well as those of R. prolixus were correctly identified. CONCLUSIONS: The morphological characters observed in the specimens of the colonies originally identified as R. prolixus and R. neglectus, such as the presence or absence of collar in the eggs, the patterns of the median process of the pygophore, and anterolateral angle, are consistent with the species. Geometric morphometrics also show an intraspecific variability in R. prolixus.
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spelling pubmed-71562572020-04-16 Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters Falcone, Rossana Ribeiro, Aline Rimoldi de Oliveira, Jader Mendonça, Vagner José Graminha, Márcia da Rosa, João Aristeu Rev Soc Bras Med Trop Major Article INTRODUCTION: The genus Rhodnius in the subfamily Triatominae comprises 20 species, which can transmit Trypanosoma cruzi and Trypanosoma rangeli. Due to the development of molecular techniques, Triatominae species can now be characterized by mitochondrial and nuclear markers, making it possible to verify and/or correct the existing data on these species. The results achieved in this study provide a more detailed and accurate differentiation of the Rhodnius species, helping the establishment of a more appropriate classification. METHODS: Data collection was performed by DNA analysis, morphological and morphometric studies to distinguish four populations of R. neglectus and four of R. prolixus. Phylogenetic data were compared to morphological and morphometric data. RESULTS: The analysis of Cytb fragments suggests that the four colonies designated to Rhodnius neglectus as well as those of R. prolixus were correctly identified. CONCLUSIONS: The morphological characters observed in the specimens of the colonies originally identified as R. prolixus and R. neglectus, such as the presence or absence of collar in the eggs, the patterns of the median process of the pygophore, and anterolateral angle, are consistent with the species. Geometric morphometrics also show an intraspecific variability in R. prolixus. Sociedade Brasileira de Medicina Tropical - SBMT 2020-04-03 /pmc/articles/PMC7156257/ /pubmed/32267457 http://dx.doi.org/10.1590/0037-8682-0503-2019 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Major Article
Falcone, Rossana
Ribeiro, Aline Rimoldi
de Oliveira, Jader
Mendonça, Vagner José
Graminha, Márcia
da Rosa, João Aristeu
Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters
title Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters
title_full Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters
title_fullStr Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters
title_full_unstemmed Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters
title_short Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters
title_sort differentiation of rhodnius neglectus and rhodnius prolixus (hemiptera: reduviidae: triatominae) by multiple parameters
topic Major Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156257/
https://www.ncbi.nlm.nih.gov/pubmed/32267457
http://dx.doi.org/10.1590/0037-8682-0503-2019
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