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Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones

Mediator of cell motility 1 (MEMO1) is a ubiquitously expressed modulator of cellular responses to growth factors including FGF23 signaling, and Memo1‐deficient mice share some phenotypic traits with Fgf23‐ or Klotho‐deficient mouse models. Here, we tested whether Memo1 gene expression is regulated...

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Detalles Bibliográficos
Autores principales: Moor, Matthias B., Bonny, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156332/
https://www.ncbi.nlm.nih.gov/pubmed/32291966
http://dx.doi.org/10.14814/phy2.14410
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author Moor, Matthias B.
Bonny, Olivier
author_facet Moor, Matthias B.
Bonny, Olivier
author_sort Moor, Matthias B.
collection PubMed
description Mediator of cell motility 1 (MEMO1) is a ubiquitously expressed modulator of cellular responses to growth factors including FGF23 signaling, and Memo1‐deficient mice share some phenotypic traits with Fgf23‐ or Klotho‐deficient mouse models. Here, we tested whether Memo1 gene expression is regulated by calciotropic hormones or by changing the dietary mineral load. MLO‐Y4 osteocyte‐like cells were cultured and treated with 1,25(OH)(2)‐vitamin D(3). Wild‐type C57BL/6N mice underwent treatments with 1,25(OH)(2)‐vitamin D(3), parathyroid hormone, 17β‐estradiol or vehicle. Other cohorts of C57BL/6N mice were fed diets varying in calcium or phosphate content. Expression of Memo1 and control genes was assessed by qPCR. 1,25(OH)(2)‐vitamin D(3) caused an acute decrease in Memo1 transcript levels in vitro, but not in vivo. None of the hormones tested had an influence on Memo1 transcripts, whereas the assessed control genes reacted the expected way. Dietary interventions with calcium and phosphate did not affect Memo1 transcripts but altered the chosen control genes’ expression. We observed that Memo1 was not regulated by calciotropic hormones or change in mineral load, suggesting major differences between the regulation and physiological roles of Klotho, Fgf23, and Memo1.
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spelling pubmed-71563322020-04-20 Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones Moor, Matthias B. Bonny, Olivier Physiol Rep Original Research Mediator of cell motility 1 (MEMO1) is a ubiquitously expressed modulator of cellular responses to growth factors including FGF23 signaling, and Memo1‐deficient mice share some phenotypic traits with Fgf23‐ or Klotho‐deficient mouse models. Here, we tested whether Memo1 gene expression is regulated by calciotropic hormones or by changing the dietary mineral load. MLO‐Y4 osteocyte‐like cells were cultured and treated with 1,25(OH)(2)‐vitamin D(3). Wild‐type C57BL/6N mice underwent treatments with 1,25(OH)(2)‐vitamin D(3), parathyroid hormone, 17β‐estradiol or vehicle. Other cohorts of C57BL/6N mice were fed diets varying in calcium or phosphate content. Expression of Memo1 and control genes was assessed by qPCR. 1,25(OH)(2)‐vitamin D(3) caused an acute decrease in Memo1 transcript levels in vitro, but not in vivo. None of the hormones tested had an influence on Memo1 transcripts, whereas the assessed control genes reacted the expected way. Dietary interventions with calcium and phosphate did not affect Memo1 transcripts but altered the chosen control genes’ expression. We observed that Memo1 was not regulated by calciotropic hormones or change in mineral load, suggesting major differences between the regulation and physiological roles of Klotho, Fgf23, and Memo1. John Wiley and Sons Inc. 2020-04-14 /pmc/articles/PMC7156332/ /pubmed/32291966 http://dx.doi.org/10.14814/phy2.14410 Text en © 2020 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Moor, Matthias B.
Bonny, Olivier
Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones
title Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones
title_full Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones
title_fullStr Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones
title_full_unstemmed Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones
title_short Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones
title_sort memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156332/
https://www.ncbi.nlm.nih.gov/pubmed/32291966
http://dx.doi.org/10.14814/phy2.14410
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