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Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current...

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Detalles Bibliográficos
Autores principales: Wang, Qin, Zhong, Chao, Xiao, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156587/
https://www.ncbi.nlm.nih.gov/pubmed/32322579
http://dx.doi.org/10.3389/fbioe.2020.00293
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author Wang, Qin
Zhong, Chao
Xiao, Han
author_facet Wang, Qin
Zhong, Chao
Xiao, Han
author_sort Wang, Qin
collection PubMed
description Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi.
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spelling pubmed-71565872020-04-22 Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion Wang, Qin Zhong, Chao Xiao, Han Front Bioeng Biotechnol Bioengineering and Biotechnology Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi. Frontiers Media S.A. 2020-04-08 /pmc/articles/PMC7156587/ /pubmed/32322579 http://dx.doi.org/10.3389/fbioe.2020.00293 Text en Copyright © 2020 Wang, Zhong and Xiao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wang, Qin
Zhong, Chao
Xiao, Han
Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion
title Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion
title_full Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion
title_fullStr Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion
title_full_unstemmed Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion
title_short Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion
title_sort genetic engineering of filamentous fungi for efficient protein expression and secretion
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156587/
https://www.ncbi.nlm.nih.gov/pubmed/32322579
http://dx.doi.org/10.3389/fbioe.2020.00293
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