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Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma

Analysis of microRNA (miRNA) regulatory networks is useful for exploring novel biomarkers and therapeutic targets in cancer cells. The Cancer Genome Atlas dataset shows that low expression of both strands of pre‐miR‐101 (miR‐101‐5p and miR‐101‐3p) significantly predicted poor prognosis in clear cell...

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Autores principales: Yamada, Yasutaka, Nohata, Nijiro, Uchida, Akifumi, Kato, Mayuko, Arai, Takayuki, Moriya, Shogo, Mizuno, Keiko, Kojima, Satoko, Yamazaki, Kazuto, Naya, Yukio, Ichikawa, Tomohiko, Seki, Naohiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156888/
https://www.ncbi.nlm.nih.gov/pubmed/31975570
http://dx.doi.org/10.1111/cas.14327
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author Yamada, Yasutaka
Nohata, Nijiro
Uchida, Akifumi
Kato, Mayuko
Arai, Takayuki
Moriya, Shogo
Mizuno, Keiko
Kojima, Satoko
Yamazaki, Kazuto
Naya, Yukio
Ichikawa, Tomohiko
Seki, Naohiko
author_facet Yamada, Yasutaka
Nohata, Nijiro
Uchida, Akifumi
Kato, Mayuko
Arai, Takayuki
Moriya, Shogo
Mizuno, Keiko
Kojima, Satoko
Yamazaki, Kazuto
Naya, Yukio
Ichikawa, Tomohiko
Seki, Naohiko
author_sort Yamada, Yasutaka
collection PubMed
description Analysis of microRNA (miRNA) regulatory networks is useful for exploring novel biomarkers and therapeutic targets in cancer cells. The Cancer Genome Atlas dataset shows that low expression of both strands of pre‐miR‐101 (miR‐101‐5p and miR‐101‐3p) significantly predicted poor prognosis in clear cell renal cell carcinoma (ccRCC). The functional significance of miR‐101‐5p in cancer cells is poorly understood. Here, we focused on miR‐101‐5p to investigate the antitumor function and its regulatory networks in ccRCC cells. Ectopic expression of mature miRNAs or siRNAs was investigated in cancer cell lines to characterize cell function, ie, proliferation, apoptosis, migration, and invasion. Genome‐wide gene expression and in silico database analyses were undertaken to predict miRNA regulatory networks. Expression of miR‐101‐5p caused cell cycle arrest and apoptosis in ccRCC cells. Downstream neighbor of son (DONSON) was directly regulated by miR‐101‐5p, and its aberrant expression was significantly associated with shorter survival in propensity score‐matched analysis (P = .0001). Knockdown of DONSON attenuated ccRCC cell aggressiveness. Several replisome genes controlled by DONSON and their expression were closely associated with ccRCC pathogenesis. The antitumor miR‐101‐5p/DONSON axis and its modulated replisome genes might be a novel diagnostic and therapeutic target for ccRCC.
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spelling pubmed-71568882020-04-20 Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma Yamada, Yasutaka Nohata, Nijiro Uchida, Akifumi Kato, Mayuko Arai, Takayuki Moriya, Shogo Mizuno, Keiko Kojima, Satoko Yamazaki, Kazuto Naya, Yukio Ichikawa, Tomohiko Seki, Naohiko Cancer Sci Original Articles Analysis of microRNA (miRNA) regulatory networks is useful for exploring novel biomarkers and therapeutic targets in cancer cells. The Cancer Genome Atlas dataset shows that low expression of both strands of pre‐miR‐101 (miR‐101‐5p and miR‐101‐3p) significantly predicted poor prognosis in clear cell renal cell carcinoma (ccRCC). The functional significance of miR‐101‐5p in cancer cells is poorly understood. Here, we focused on miR‐101‐5p to investigate the antitumor function and its regulatory networks in ccRCC cells. Ectopic expression of mature miRNAs or siRNAs was investigated in cancer cell lines to characterize cell function, ie, proliferation, apoptosis, migration, and invasion. Genome‐wide gene expression and in silico database analyses were undertaken to predict miRNA regulatory networks. Expression of miR‐101‐5p caused cell cycle arrest and apoptosis in ccRCC cells. Downstream neighbor of son (DONSON) was directly regulated by miR‐101‐5p, and its aberrant expression was significantly associated with shorter survival in propensity score‐matched analysis (P = .0001). Knockdown of DONSON attenuated ccRCC cell aggressiveness. Several replisome genes controlled by DONSON and their expression were closely associated with ccRCC pathogenesis. The antitumor miR‐101‐5p/DONSON axis and its modulated replisome genes might be a novel diagnostic and therapeutic target for ccRCC. John Wiley and Sons Inc. 2020-02-22 2020-04 /pmc/articles/PMC7156888/ /pubmed/31975570 http://dx.doi.org/10.1111/cas.14327 Text en © 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Yamada, Yasutaka
Nohata, Nijiro
Uchida, Akifumi
Kato, Mayuko
Arai, Takayuki
Moriya, Shogo
Mizuno, Keiko
Kojima, Satoko
Yamazaki, Kazuto
Naya, Yukio
Ichikawa, Tomohiko
Seki, Naohiko
Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma
title Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma
title_full Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma
title_fullStr Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma
title_full_unstemmed Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma
title_short Replisome genes regulation by antitumor miR‐101‐5p in clear cell renal cell carcinoma
title_sort replisome genes regulation by antitumor mir‐101‐5p in clear cell renal cell carcinoma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156888/
https://www.ncbi.nlm.nih.gov/pubmed/31975570
http://dx.doi.org/10.1111/cas.14327
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