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Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy

Bacterial pathogens cause severe infections worldwide in livestock and in humans, and antibiotic resistance further increases the importance of prophylactic vaccines. Inactivated bacterial vaccines (bacterins) are usually produced via incubation of the pathogen with chemicals such as formaldehyde, w...

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Autores principales: Fertey, Jasmin, Bayer, Lea, Kähl, Sophie, Haji, Rukiya M., Burger-Kentischer, Anke, Thoma, Martin, Standfest, Bastian, Schönfelder, Jessy, Portillo Casado, Javier, Rögner, Frank-Holm, Baums, Christoph Georg, Grunwald, Thomas, Ulbert, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157226/
https://www.ncbi.nlm.nih.gov/pubmed/32121656
http://dx.doi.org/10.3390/vaccines8010113
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author Fertey, Jasmin
Bayer, Lea
Kähl, Sophie
Haji, Rukiya M.
Burger-Kentischer, Anke
Thoma, Martin
Standfest, Bastian
Schönfelder, Jessy
Portillo Casado, Javier
Rögner, Frank-Holm
Baums, Christoph Georg
Grunwald, Thomas
Ulbert, Sebastian
author_facet Fertey, Jasmin
Bayer, Lea
Kähl, Sophie
Haji, Rukiya M.
Burger-Kentischer, Anke
Thoma, Martin
Standfest, Bastian
Schönfelder, Jessy
Portillo Casado, Javier
Rögner, Frank-Holm
Baums, Christoph Georg
Grunwald, Thomas
Ulbert, Sebastian
author_sort Fertey, Jasmin
collection PubMed
description Bacterial pathogens cause severe infections worldwide in livestock and in humans, and antibiotic resistance further increases the importance of prophylactic vaccines. Inactivated bacterial vaccines (bacterins) are usually produced via incubation of the pathogen with chemicals such as formaldehyde, which is time consuming and may cause loss of immunogenicity due to the modification of structural components. We evaluated low-energy electron irradiation (LEEI) as an alternative method to generate a bacterin. Rodentibacter pneumotropicus, an invasive Gram-negative murine pathogen, was inactivated with LEEI and formaldehyde. LEEI resulted in high antigen conservation, and LPS activity was significantly better maintained when compared with formaldehyde treatment. Immunization of mice with LEEI-inactivated R. pneumotropicus elicited a strong immune response with no detectable bacterial burden upon sublethal challenge. The results of this study suggest the inactivation of bacteria with LEEI as an alternative, fast and efficient method to generate bacterial vaccines with increased efficacy.
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spelling pubmed-71572262020-05-01 Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy Fertey, Jasmin Bayer, Lea Kähl, Sophie Haji, Rukiya M. Burger-Kentischer, Anke Thoma, Martin Standfest, Bastian Schönfelder, Jessy Portillo Casado, Javier Rögner, Frank-Holm Baums, Christoph Georg Grunwald, Thomas Ulbert, Sebastian Vaccines (Basel) Article Bacterial pathogens cause severe infections worldwide in livestock and in humans, and antibiotic resistance further increases the importance of prophylactic vaccines. Inactivated bacterial vaccines (bacterins) are usually produced via incubation of the pathogen with chemicals such as formaldehyde, which is time consuming and may cause loss of immunogenicity due to the modification of structural components. We evaluated low-energy electron irradiation (LEEI) as an alternative method to generate a bacterin. Rodentibacter pneumotropicus, an invasive Gram-negative murine pathogen, was inactivated with LEEI and formaldehyde. LEEI resulted in high antigen conservation, and LPS activity was significantly better maintained when compared with formaldehyde treatment. Immunization of mice with LEEI-inactivated R. pneumotropicus elicited a strong immune response with no detectable bacterial burden upon sublethal challenge. The results of this study suggest the inactivation of bacteria with LEEI as an alternative, fast and efficient method to generate bacterial vaccines with increased efficacy. MDPI 2020-03-02 /pmc/articles/PMC7157226/ /pubmed/32121656 http://dx.doi.org/10.3390/vaccines8010113 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fertey, Jasmin
Bayer, Lea
Kähl, Sophie
Haji, Rukiya M.
Burger-Kentischer, Anke
Thoma, Martin
Standfest, Bastian
Schönfelder, Jessy
Portillo Casado, Javier
Rögner, Frank-Holm
Baums, Christoph Georg
Grunwald, Thomas
Ulbert, Sebastian
Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
title Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
title_full Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
title_fullStr Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
title_full_unstemmed Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
title_short Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
title_sort low-energy electron irradiation efficiently inactivates the gram-negative pathogen rodentibacter pneumotropicus—a new method for the generation of bacterial vaccines with increased efficacy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157226/
https://www.ncbi.nlm.nih.gov/pubmed/32121656
http://dx.doi.org/10.3390/vaccines8010113
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