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Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets
Legionella pneumophila governs its interactions with host cells by secreting >300 different “effector” proteins. Some of these effectors contain eukaryotic domains such as the RCC1 (regulator of chromosome condensation 1) repeats promoting the activation of the small GTPase Ran. In this report, w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157520/ https://www.ncbi.nlm.nih.gov/pubmed/32209684 http://dx.doi.org/10.1128/mBio.00405-20 |
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author | Swart, A. Leoni Steiner, Bernhard Gomez-Valero, Laura Schütz, Sabina Hannemann, Mandy Janning, Petra Irminger, Michael Rothmeier, Eva Buchrieser, Carmen Itzen, Aymelt Panse, Vikram Govind Hilbi, Hubert |
author_facet | Swart, A. Leoni Steiner, Bernhard Gomez-Valero, Laura Schütz, Sabina Hannemann, Mandy Janning, Petra Irminger, Michael Rothmeier, Eva Buchrieser, Carmen Itzen, Aymelt Panse, Vikram Govind Hilbi, Hubert |
author_sort | Swart, A. Leoni |
collection | PubMed |
description | Legionella pneumophila governs its interactions with host cells by secreting >300 different “effector” proteins. Some of these effectors contain eukaryotic domains such as the RCC1 (regulator of chromosome condensation 1) repeats promoting the activation of the small GTPase Ran. In this report, we reveal a conserved pattern of L. pneumophila RCC1 repeat genes, which are distributed in two main clusters of strains. Accordingly, strain Philadelphia-1 contains two RCC1 genes implicated in bacterial virulence, legG1 (Legionella eukaryotic gene 1), and ppgA, while strain Paris contains only one, pieG. The RCC1 repeat effectors localize to different cellular compartments and bind distinct components of the Ran GTPase cycle, including Ran modulators and the small GTPase itself, and yet they all promote the activation of Ran. The pieG gene spans the corresponding open reading frames of legG1 and a separate adjacent upstream gene, lpg1975. legG1 and lpg1975 are fused upon addition of a single nucleotide to encode a protein that adopts the binding specificity of PieG. Thus, a point mutation in pieG splits the gene, altering the effector target. These results indicate that divergent evolution of RCC1 repeat effectors defines the Ran GTPase cycle targets and that modulation of different components of the cycle might fine-tune Ran activation during Legionella infection. |
format | Online Article Text |
id | pubmed-7157520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-71575202020-04-15 Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets Swart, A. Leoni Steiner, Bernhard Gomez-Valero, Laura Schütz, Sabina Hannemann, Mandy Janning, Petra Irminger, Michael Rothmeier, Eva Buchrieser, Carmen Itzen, Aymelt Panse, Vikram Govind Hilbi, Hubert mBio Research Article Legionella pneumophila governs its interactions with host cells by secreting >300 different “effector” proteins. Some of these effectors contain eukaryotic domains such as the RCC1 (regulator of chromosome condensation 1) repeats promoting the activation of the small GTPase Ran. In this report, we reveal a conserved pattern of L. pneumophila RCC1 repeat genes, which are distributed in two main clusters of strains. Accordingly, strain Philadelphia-1 contains two RCC1 genes implicated in bacterial virulence, legG1 (Legionella eukaryotic gene 1), and ppgA, while strain Paris contains only one, pieG. The RCC1 repeat effectors localize to different cellular compartments and bind distinct components of the Ran GTPase cycle, including Ran modulators and the small GTPase itself, and yet they all promote the activation of Ran. The pieG gene spans the corresponding open reading frames of legG1 and a separate adjacent upstream gene, lpg1975. legG1 and lpg1975 are fused upon addition of a single nucleotide to encode a protein that adopts the binding specificity of PieG. Thus, a point mutation in pieG splits the gene, altering the effector target. These results indicate that divergent evolution of RCC1 repeat effectors defines the Ran GTPase cycle targets and that modulation of different components of the cycle might fine-tune Ran activation during Legionella infection. American Society for Microbiology 2020-03-24 /pmc/articles/PMC7157520/ /pubmed/32209684 http://dx.doi.org/10.1128/mBio.00405-20 Text en Copyright © 2020 Swart et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Swart, A. Leoni Steiner, Bernhard Gomez-Valero, Laura Schütz, Sabina Hannemann, Mandy Janning, Petra Irminger, Michael Rothmeier, Eva Buchrieser, Carmen Itzen, Aymelt Panse, Vikram Govind Hilbi, Hubert Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets |
title | Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets |
title_full | Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets |
title_fullStr | Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets |
title_full_unstemmed | Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets |
title_short | Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets |
title_sort | divergent evolution of legionella rcc1 repeat effectors defines the range of ran gtpase cycle targets |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157520/ https://www.ncbi.nlm.nih.gov/pubmed/32209684 http://dx.doi.org/10.1128/mBio.00405-20 |
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