Cargando…

Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli

Bacterial flagellar motility plays an important role in many processes that occur at surfaces or in hydrogels, including adhesion, biofilm formation, and bacterium-host interactions. Consequently, expression of flagellar genes, as well as genes involved in biofilm formation and virulence, can be reg...

Descripción completa

Detalles Bibliográficos
Autores principales: Laganenka, Leanid, López, María Esteban, Colin, Remy, Sourjik, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157525/
https://www.ncbi.nlm.nih.gov/pubmed/32209689
http://dx.doi.org/10.1128/mBio.02269-19
_version_ 1783522362263601152
author Laganenka, Leanid
López, María Esteban
Colin, Remy
Sourjik, Victor
author_facet Laganenka, Leanid
López, María Esteban
Colin, Remy
Sourjik, Victor
author_sort Laganenka, Leanid
collection PubMed
description Bacterial flagellar motility plays an important role in many processes that occur at surfaces or in hydrogels, including adhesion, biofilm formation, and bacterium-host interactions. Consequently, expression of flagellar genes, as well as genes involved in biofilm formation and virulence, can be regulated by the surface contact. In a few bacterial species, flagella themselves are known to serve as mechanosensors, where an increased load on flagella experienced during surface contact or swimming in viscous media controls gene expression. In this study, we show that gene regulation by motility-dependent mechanosensing is common among pathogenic Escherichia coli strains. This regulatory mechanism requires flagellar rotation, and it enables pathogenic E. coli to repress flagellar genes at low loads in liquid culture, while activating motility in porous medium (soft agar) or upon surface contact. It also controls several other cellular functions, including metabolism and signaling. The mechanosensing response in pathogenic E. coli depends on the negative regulator of motility, RflP (YdiV), which inhibits basal expression of flagellar genes in liquid. While no conditional inhibition of flagellar gene expression in liquid and therefore no upregulation in porous medium was observed in the wild-type commensal or laboratory strains of E. coli, mechanosensitive regulation could be recovered by overexpression of RflP in the laboratory strain. We hypothesize that this conditional activation of flagellar genes in pathogenic E. coli reflects adaptation to the dual role played by flagella and motility during infection.
format Online
Article
Text
id pubmed-7157525
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-71575252020-04-15 Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli Laganenka, Leanid López, María Esteban Colin, Remy Sourjik, Victor mBio Observation Bacterial flagellar motility plays an important role in many processes that occur at surfaces or in hydrogels, including adhesion, biofilm formation, and bacterium-host interactions. Consequently, expression of flagellar genes, as well as genes involved in biofilm formation and virulence, can be regulated by the surface contact. In a few bacterial species, flagella themselves are known to serve as mechanosensors, where an increased load on flagella experienced during surface contact or swimming in viscous media controls gene expression. In this study, we show that gene regulation by motility-dependent mechanosensing is common among pathogenic Escherichia coli strains. This regulatory mechanism requires flagellar rotation, and it enables pathogenic E. coli to repress flagellar genes at low loads in liquid culture, while activating motility in porous medium (soft agar) or upon surface contact. It also controls several other cellular functions, including metabolism and signaling. The mechanosensing response in pathogenic E. coli depends on the negative regulator of motility, RflP (YdiV), which inhibits basal expression of flagellar genes in liquid. While no conditional inhibition of flagellar gene expression in liquid and therefore no upregulation in porous medium was observed in the wild-type commensal or laboratory strains of E. coli, mechanosensitive regulation could be recovered by overexpression of RflP in the laboratory strain. We hypothesize that this conditional activation of flagellar genes in pathogenic E. coli reflects adaptation to the dual role played by flagella and motility during infection. American Society for Microbiology 2020-03-24 /pmc/articles/PMC7157525/ /pubmed/32209689 http://dx.doi.org/10.1128/mBio.02269-19 Text en Copyright © 2020 Laganenka et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Observation
Laganenka, Leanid
López, María Esteban
Colin, Remy
Sourjik, Victor
Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
title Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
title_full Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
title_fullStr Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
title_full_unstemmed Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
title_short Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
title_sort flagellum-mediated mechanosensing and rflp control motility state of pathogenic escherichia coli
topic Observation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157525/
https://www.ncbi.nlm.nih.gov/pubmed/32209689
http://dx.doi.org/10.1128/mBio.02269-19
work_keys_str_mv AT laganenkaleanid flagellummediatedmechanosensingandrflpcontrolmotilitystateofpathogenicescherichiacoli
AT lopezmariaesteban flagellummediatedmechanosensingandrflpcontrolmotilitystateofpathogenicescherichiacoli
AT colinremy flagellummediatedmechanosensingandrflpcontrolmotilitystateofpathogenicescherichiacoli
AT sourjikvictor flagellummediatedmechanosensingandrflpcontrolmotilitystateofpathogenicescherichiacoli