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Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
Bacterial flagellar motility plays an important role in many processes that occur at surfaces or in hydrogels, including adhesion, biofilm formation, and bacterium-host interactions. Consequently, expression of flagellar genes, as well as genes involved in biofilm formation and virulence, can be reg...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157525/ https://www.ncbi.nlm.nih.gov/pubmed/32209689 http://dx.doi.org/10.1128/mBio.02269-19 |
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author | Laganenka, Leanid López, María Esteban Colin, Remy Sourjik, Victor |
author_facet | Laganenka, Leanid López, María Esteban Colin, Remy Sourjik, Victor |
author_sort | Laganenka, Leanid |
collection | PubMed |
description | Bacterial flagellar motility plays an important role in many processes that occur at surfaces or in hydrogels, including adhesion, biofilm formation, and bacterium-host interactions. Consequently, expression of flagellar genes, as well as genes involved in biofilm formation and virulence, can be regulated by the surface contact. In a few bacterial species, flagella themselves are known to serve as mechanosensors, where an increased load on flagella experienced during surface contact or swimming in viscous media controls gene expression. In this study, we show that gene regulation by motility-dependent mechanosensing is common among pathogenic Escherichia coli strains. This regulatory mechanism requires flagellar rotation, and it enables pathogenic E. coli to repress flagellar genes at low loads in liquid culture, while activating motility in porous medium (soft agar) or upon surface contact. It also controls several other cellular functions, including metabolism and signaling. The mechanosensing response in pathogenic E. coli depends on the negative regulator of motility, RflP (YdiV), which inhibits basal expression of flagellar genes in liquid. While no conditional inhibition of flagellar gene expression in liquid and therefore no upregulation in porous medium was observed in the wild-type commensal or laboratory strains of E. coli, mechanosensitive regulation could be recovered by overexpression of RflP in the laboratory strain. We hypothesize that this conditional activation of flagellar genes in pathogenic E. coli reflects adaptation to the dual role played by flagella and motility during infection. |
format | Online Article Text |
id | pubmed-7157525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-71575252020-04-15 Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli Laganenka, Leanid López, María Esteban Colin, Remy Sourjik, Victor mBio Observation Bacterial flagellar motility plays an important role in many processes that occur at surfaces or in hydrogels, including adhesion, biofilm formation, and bacterium-host interactions. Consequently, expression of flagellar genes, as well as genes involved in biofilm formation and virulence, can be regulated by the surface contact. In a few bacterial species, flagella themselves are known to serve as mechanosensors, where an increased load on flagella experienced during surface contact or swimming in viscous media controls gene expression. In this study, we show that gene regulation by motility-dependent mechanosensing is common among pathogenic Escherichia coli strains. This regulatory mechanism requires flagellar rotation, and it enables pathogenic E. coli to repress flagellar genes at low loads in liquid culture, while activating motility in porous medium (soft agar) or upon surface contact. It also controls several other cellular functions, including metabolism and signaling. The mechanosensing response in pathogenic E. coli depends on the negative regulator of motility, RflP (YdiV), which inhibits basal expression of flagellar genes in liquid. While no conditional inhibition of flagellar gene expression in liquid and therefore no upregulation in porous medium was observed in the wild-type commensal or laboratory strains of E. coli, mechanosensitive regulation could be recovered by overexpression of RflP in the laboratory strain. We hypothesize that this conditional activation of flagellar genes in pathogenic E. coli reflects adaptation to the dual role played by flagella and motility during infection. American Society for Microbiology 2020-03-24 /pmc/articles/PMC7157525/ /pubmed/32209689 http://dx.doi.org/10.1128/mBio.02269-19 Text en Copyright © 2020 Laganenka et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Observation Laganenka, Leanid López, María Esteban Colin, Remy Sourjik, Victor Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli |
title | Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli |
title_full | Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli |
title_fullStr | Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli |
title_full_unstemmed | Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli |
title_short | Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli |
title_sort | flagellum-mediated mechanosensing and rflp control motility state of pathogenic escherichia coli |
topic | Observation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157525/ https://www.ncbi.nlm.nih.gov/pubmed/32209689 http://dx.doi.org/10.1128/mBio.02269-19 |
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