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Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform

Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic alp...

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Autores principales: Kimpston-Burkgren, Kay, Mora-Díaz, Juan Carlos, Roby, Philippe, Bjustrom-Kraft, Jordan, Main, Rodger, Bosse, Roger, Giménez-Lirola, Luis Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157568/
https://www.ncbi.nlm.nih.gov/pubmed/32245150
http://dx.doi.org/10.3390/pathogens9030233
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author Kimpston-Burkgren, Kay
Mora-Díaz, Juan Carlos
Roby, Philippe
Bjustrom-Kraft, Jordan
Main, Rodger
Bosse, Roger
Giménez-Lirola, Luis Gabriel
author_facet Kimpston-Burkgren, Kay
Mora-Díaz, Juan Carlos
Roby, Philippe
Bjustrom-Kraft, Jordan
Main, Rodger
Bosse, Roger
Giménez-Lirola, Luis Gabriel
author_sort Kimpston-Burkgren, Kay
collection PubMed
description Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic alphacoronavirus PED virus (PEDV). In the absence of effective vaccines, infection prevention and control through diagnostic testing and quarantine are critical. Early detection and differential diagnosis of PEDV infections increase the chance of successful control of the disease. Therefore, there is a continuous need for development of reduced assay-step protocols, no-wash, high-throughput immunoassays. This study described the characterization of the humoral immune response against PEDV under experimental and field conditions using a rapid, sensitive, luminescent proximity homogenous assay (AlphaLISA). PEDV IgG and IgA antibodies were developed toward the beginning of the second week of infection. PEDV IgG antibodies were detected for at least 16 weeks post-exposure. Remarkably, the serum IgA levels remained high and relatively stable throughout the study, lasting longer than the serum IgG response. Overall, AlphaLISA allows the detection and characterization of pathogen-specific antibodies with new speed, sensitivity, and simplicity of use. Particularly, the bridge assay constitutes a rapid diagnostic that substantially improves upon the “time to result” metric of currently available immunoassays.
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spelling pubmed-71575682020-05-01 Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform Kimpston-Burkgren, Kay Mora-Díaz, Juan Carlos Roby, Philippe Bjustrom-Kraft, Jordan Main, Rodger Bosse, Roger Giménez-Lirola, Luis Gabriel Pathogens Article Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic alphacoronavirus PED virus (PEDV). In the absence of effective vaccines, infection prevention and control through diagnostic testing and quarantine are critical. Early detection and differential diagnosis of PEDV infections increase the chance of successful control of the disease. Therefore, there is a continuous need for development of reduced assay-step protocols, no-wash, high-throughput immunoassays. This study described the characterization of the humoral immune response against PEDV under experimental and field conditions using a rapid, sensitive, luminescent proximity homogenous assay (AlphaLISA). PEDV IgG and IgA antibodies were developed toward the beginning of the second week of infection. PEDV IgG antibodies were detected for at least 16 weeks post-exposure. Remarkably, the serum IgA levels remained high and relatively stable throughout the study, lasting longer than the serum IgG response. Overall, AlphaLISA allows the detection and characterization of pathogen-specific antibodies with new speed, sensitivity, and simplicity of use. Particularly, the bridge assay constitutes a rapid diagnostic that substantially improves upon the “time to result” metric of currently available immunoassays. MDPI 2020-03-21 /pmc/articles/PMC7157568/ /pubmed/32245150 http://dx.doi.org/10.3390/pathogens9030233 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kimpston-Burkgren, Kay
Mora-Díaz, Juan Carlos
Roby, Philippe
Bjustrom-Kraft, Jordan
Main, Rodger
Bosse, Roger
Giménez-Lirola, Luis Gabriel
Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform
title Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform
title_full Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform
title_fullStr Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform
title_full_unstemmed Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform
title_short Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform
title_sort characterization of the humoral immune response to porcine epidemic diarrhea virus infection under experimental and field conditions using an alphalisa platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157568/
https://www.ncbi.nlm.nih.gov/pubmed/32245150
http://dx.doi.org/10.3390/pathogens9030233
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