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Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae

Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. cor...

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Autores principales: Kim, Hyoun Joong, Jun, Jin Woo, Giri, Sib Sankar, Chi, Cheng, Yun, Saekil, Kim, Sang Guen, Kim, Sang Wha, Han, Se Jin, Kwon, Jun, Oh, Woo Taek, Lee, Sung Bin, Kim, Ji Hyung, Park, Se Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157721/
https://www.ncbi.nlm.nih.gov/pubmed/32168754
http://dx.doi.org/10.3390/pathogens9030206
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author Kim, Hyoun Joong
Jun, Jin Woo
Giri, Sib Sankar
Chi, Cheng
Yun, Saekil
Kim, Sang Guen
Kim, Sang Wha
Han, Se Jin
Kwon, Jun
Oh, Woo Taek
Lee, Sung Bin
Kim, Ji Hyung
Park, Se Chang
author_facet Kim, Hyoun Joong
Jun, Jin Woo
Giri, Sib Sankar
Chi, Cheng
Yun, Saekil
Kim, Sang Guen
Kim, Sang Wha
Han, Se Jin
Kwon, Jun
Oh, Woo Taek
Lee, Sung Bin
Kim, Ji Hyung
Park, Se Chang
author_sort Kim, Hyoun Joong
collection PubMed
description Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 × 10(5) CFU/mL for 24 h caused mortality to 88.65 ± 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to β-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors.
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spelling pubmed-71577212020-04-21 Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae Kim, Hyoun Joong Jun, Jin Woo Giri, Sib Sankar Chi, Cheng Yun, Saekil Kim, Sang Guen Kim, Sang Wha Han, Se Jin Kwon, Jun Oh, Woo Taek Lee, Sung Bin Kim, Ji Hyung Park, Se Chang Pathogens Article Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 × 10(5) CFU/mL for 24 h caused mortality to 88.65 ± 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to β-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors. MDPI 2020-03-11 /pmc/articles/PMC7157721/ /pubmed/32168754 http://dx.doi.org/10.3390/pathogens9030206 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Hyoun Joong
Jun, Jin Woo
Giri, Sib Sankar
Chi, Cheng
Yun, Saekil
Kim, Sang Guen
Kim, Sang Wha
Han, Se Jin
Kwon, Jun
Oh, Woo Taek
Lee, Sung Bin
Kim, Ji Hyung
Park, Se Chang
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
title Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
title_full Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
title_fullStr Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
title_full_unstemmed Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
title_short Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
title_sort identification and genome analysis of vibrio coralliilyticus causing mortality of pacific oyster (crassostrea gigas) larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157721/
https://www.ncbi.nlm.nih.gov/pubmed/32168754
http://dx.doi.org/10.3390/pathogens9030206
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