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Gnotobiotics and the Microbiome
This chapter provides an overview of germfree (GF), gnotobiotic (GN), and defined flora (DF) laboratory rats, relating their history, traditional and modern derivation procedures, the anatomy and physiology, and their use in the study of mammalian host–microbiome relationships. Extensive literature...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158190/ http://dx.doi.org/10.1016/B978-0-12-814338-4.00021-0 |
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author | Carter, Philip B. Norin, Elisabeth Swennes, Alton G. |
author_facet | Carter, Philip B. Norin, Elisabeth Swennes, Alton G. |
author_sort | Carter, Philip B. |
collection | PubMed |
description | This chapter provides an overview of germfree (GF), gnotobiotic (GN), and defined flora (DF) laboratory rats, relating their history, traditional and modern derivation procedures, the anatomy and physiology, and their use in the study of mammalian host–microbiome relationships. Extensive literature on the nutrition and physiology of GF rats and the expanding library of immunological reagents have increased the research utility of GF, GN, or DF rats. Such rats have been extensively used in metabolic experiments as nucleus seed stocks for the production of disease-free animals and as tools for infectious disease studies, among others. The chapter also presents research applications of GF rats that are particularly suitable for testing candidate viral carcinogens since they are uniquely free of all known viruses, for pathology studies in the distinguishing of primary mediation lesions from those associated with infections, and the study of the biological effects of radiation. |
format | Online Article Text |
id | pubmed-7158190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71581902020-04-15 Gnotobiotics and the Microbiome Carter, Philip B. Norin, Elisabeth Swennes, Alton G. The Laboratory Rat Article This chapter provides an overview of germfree (GF), gnotobiotic (GN), and defined flora (DF) laboratory rats, relating their history, traditional and modern derivation procedures, the anatomy and physiology, and their use in the study of mammalian host–microbiome relationships. Extensive literature on the nutrition and physiology of GF rats and the expanding library of immunological reagents have increased the research utility of GF, GN, or DF rats. Such rats have been extensively used in metabolic experiments as nucleus seed stocks for the production of disease-free animals and as tools for infectious disease studies, among others. The chapter also presents research applications of GF rats that are particularly suitable for testing candidate viral carcinogens since they are uniquely free of all known viruses, for pathology studies in the distinguishing of primary mediation lesions from those associated with infections, and the study of the biological effects of radiation. 2020 2019-11-15 /pmc/articles/PMC7158190/ http://dx.doi.org/10.1016/B978-0-12-814338-4.00021-0 Text en Copyright © 2020 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Carter, Philip B. Norin, Elisabeth Swennes, Alton G. Gnotobiotics and the Microbiome |
title | Gnotobiotics and the Microbiome |
title_full | Gnotobiotics and the Microbiome |
title_fullStr | Gnotobiotics and the Microbiome |
title_full_unstemmed | Gnotobiotics and the Microbiome |
title_short | Gnotobiotics and the Microbiome |
title_sort | gnotobiotics and the microbiome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158190/ http://dx.doi.org/10.1016/B978-0-12-814338-4.00021-0 |
work_keys_str_mv | AT carterphilipb gnotobioticsandthemicrobiome AT norinelisabeth gnotobioticsandthemicrobiome AT swennesaltong gnotobioticsandthemicrobiome |