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Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using opti...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7159973/ https://www.ncbi.nlm.nih.gov/pubmed/32549921 http://dx.doi.org/10.1063/5.0006264 |
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author | Yamamoto, Ken Sakurai, Ryosuke Motosuke, Masahiro |
author_facet | Yamamoto, Ken Sakurai, Ryosuke Motosuke, Masahiro |
author_sort | Yamamoto, Ken |
collection | PubMed |
description | A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using optics such as a camera and detector. This high sensitivity is achieved by implementing 50-μm-thick reaction chambers in which a clear contrast between the RBC agglutinations and non-reacted RBCs can be obtained. The dilution and the homogenization are enhanced by injecting bubbles into the microchannel so that the test result can be obtained 5 min after the test start. With an assumption that the device will be used by medical staffs, the device is designed to require minimum operation for the users, namely, loading whole blood, starting pumps, and looking inside the reaction chambers by their eyes to observe the test result. As the device is applicable to the cross-matching test by mixing RBCs with serum instead of the reagents, it is expected that the device provides not only the quick blood-typing but also a safer and quicker blood transfusion in emergency rooms. |
format | Online Article Text |
id | pubmed-7159973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-71599732020-12-16 Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection Yamamoto, Ken Sakurai, Ryosuke Motosuke, Masahiro Biomicrofluidics Regular Articles A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using optics such as a camera and detector. This high sensitivity is achieved by implementing 50-μm-thick reaction chambers in which a clear contrast between the RBC agglutinations and non-reacted RBCs can be obtained. The dilution and the homogenization are enhanced by injecting bubbles into the microchannel so that the test result can be obtained 5 min after the test start. With an assumption that the device will be used by medical staffs, the device is designed to require minimum operation for the users, namely, loading whole blood, starting pumps, and looking inside the reaction chambers by their eyes to observe the test result. As the device is applicable to the cross-matching test by mixing RBCs with serum instead of the reagents, it is expected that the device provides not only the quick blood-typing but also a safer and quicker blood transfusion in emergency rooms. AIP Publishing LLC 2020-04-14 /pmc/articles/PMC7159973/ /pubmed/32549921 http://dx.doi.org/10.1063/5.0006264 Text en © 2020 Author(s). 0021-8979/2020/14(2)/024111/8/ All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Regular Articles Yamamoto, Ken Sakurai, Ryosuke Motosuke, Masahiro Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection |
title | Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection |
title_full | Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection |
title_fullStr | Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection |
title_full_unstemmed | Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection |
title_short | Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection |
title_sort | fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7159973/ https://www.ncbi.nlm.nih.gov/pubmed/32549921 http://dx.doi.org/10.1063/5.0006264 |
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