Cargando…

Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection

A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using opti...

Descripción completa

Detalles Bibliográficos
Autores principales: Yamamoto, Ken, Sakurai, Ryosuke, Motosuke, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7159973/
https://www.ncbi.nlm.nih.gov/pubmed/32549921
http://dx.doi.org/10.1063/5.0006264
_version_ 1783522676410679296
author Yamamoto, Ken
Sakurai, Ryosuke
Motosuke, Masahiro
author_facet Yamamoto, Ken
Sakurai, Ryosuke
Motosuke, Masahiro
author_sort Yamamoto, Ken
collection PubMed
description A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using optics such as a camera and detector. This high sensitivity is achieved by implementing 50-μm-thick reaction chambers in which a clear contrast between the RBC agglutinations and non-reacted RBCs can be obtained. The dilution and the homogenization are enhanced by injecting bubbles into the microchannel so that the test result can be obtained 5 min after the test start. With an assumption that the device will be used by medical staffs, the device is designed to require minimum operation for the users, namely, loading whole blood, starting pumps, and looking inside the reaction chambers by their eyes to observe the test result. As the device is applicable to the cross-matching test by mixing RBCs with serum instead of the reagents, it is expected that the device provides not only the quick blood-typing but also a safer and quicker blood transfusion in emergency rooms.
format Online
Article
Text
id pubmed-7159973
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher AIP Publishing LLC
record_format MEDLINE/PubMed
spelling pubmed-71599732020-12-16 Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection Yamamoto, Ken Sakurai, Ryosuke Motosuke, Masahiro Biomicrofluidics Regular Articles A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using optics such as a camera and detector. This high sensitivity is achieved by implementing 50-μm-thick reaction chambers in which a clear contrast between the RBC agglutinations and non-reacted RBCs can be obtained. The dilution and the homogenization are enhanced by injecting bubbles into the microchannel so that the test result can be obtained 5 min after the test start. With an assumption that the device will be used by medical staffs, the device is designed to require minimum operation for the users, namely, loading whole blood, starting pumps, and looking inside the reaction chambers by their eyes to observe the test result. As the device is applicable to the cross-matching test by mixing RBCs with serum instead of the reagents, it is expected that the device provides not only the quick blood-typing but also a safer and quicker blood transfusion in emergency rooms. AIP Publishing LLC 2020-04-14 /pmc/articles/PMC7159973/ /pubmed/32549921 http://dx.doi.org/10.1063/5.0006264 Text en © 2020 Author(s). 0021-8979/2020/14(2)/024111/8/ All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular Articles
Yamamoto, Ken
Sakurai, Ryosuke
Motosuke, Masahiro
Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
title Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
title_full Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
title_fullStr Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
title_full_unstemmed Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
title_short Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
title_sort fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7159973/
https://www.ncbi.nlm.nih.gov/pubmed/32549921
http://dx.doi.org/10.1063/5.0006264
work_keys_str_mv AT yamamotoken fullyautomaticbloodtypingchipexploitingbubblesforquickdilutionanddetection
AT sakurairyosuke fullyautomaticbloodtypingchipexploitingbubblesforquickdilutionanddetection
AT motosukemasahiro fullyautomaticbloodtypingchipexploitingbubblesforquickdilutionanddetection