An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020

The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI...

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Detalles Bibliográficos
Autores principales: Fomsgaard, Anna S., Rosenstierne, Maiken Worsøe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160440/
https://www.ncbi.nlm.nih.gov/pubmed/32290902
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.14.2000398
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author Fomsgaard, Anna S.
Rosenstierne, Maiken Worsøe
author_facet Fomsgaard, Anna S.
Rosenstierne, Maiken Worsøe
author_sort Fomsgaard, Anna S.
collection PubMed
description The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2–99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.
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spelling pubmed-71604402020-04-21 An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020 Fomsgaard, Anna S. Rosenstierne, Maiken Worsøe Euro Surveill Rapid Communication The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2–99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure. European Centre for Disease Prevention and Control (ECDC) 2020-04-09 /pmc/articles/PMC7160440/ /pubmed/32290902 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.14.2000398 Text en This article is copyright of the authors or their affiliated institutions, 2020. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Fomsgaard, Anna S.
Rosenstierne, Maiken Worsøe
An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
title An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
title_full An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
title_fullStr An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
title_full_unstemmed An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
title_short An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
title_sort alternative workflow for molecular detection of sars-cov-2 – escape from the na extraction kit-shortage, copenhagen, denmark, march 2020
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160440/
https://www.ncbi.nlm.nih.gov/pubmed/32290902
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.14.2000398
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