Cargando…

Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas)

Green turtles (Chelonia mydas) have a hindgut fermentation digestive tract, which uses cellulolytic microbes to break down plant matter in the cecum and proximal colon. Previous studies on bacterial communities of green turtles have not identified in situ hindgut microbiota, and never before in Hawa...

Descripción completa

Detalles Bibliográficos
Autores principales: McDermid, Karla J, Kittle, Ronald P, Veillet, Anne, Plouviez, Sophie, Muehlstein, Lisa, Balazs, George H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160772/
https://www.ncbi.nlm.nih.gov/pubmed/32313421
http://dx.doi.org/10.1177/1176934320914603
_version_ 1783522818947809280
author McDermid, Karla J
Kittle, Ronald P
Veillet, Anne
Plouviez, Sophie
Muehlstein, Lisa
Balazs, George H
author_facet McDermid, Karla J
Kittle, Ronald P
Veillet, Anne
Plouviez, Sophie
Muehlstein, Lisa
Balazs, George H
author_sort McDermid, Karla J
collection PubMed
description Green turtles (Chelonia mydas) have a hindgut fermentation digestive tract, which uses cellulolytic microbes to break down plant matter in the cecum and proximal colon. Previous studies on bacterial communities of green turtles have not identified in situ hindgut microbiota, and never before in Hawaiian green turtles, which comprise an isolated metapopulation. Fresh samples using sterile swabs were taken from five locations along the gastrointestinal tracts of eight green turtles that had required euthanization. Bacteria were cultured, aerobically and anaerobically, on nutrient agar and four differential and selective media. Samples at three sections along the gastrointestinal tracts of two green turtles were analyzed using 16S metagenomics on an Ion Torrent Personal Genome Machine. More than half of the 4 532 104 sequences belonged to the phylum Firmicutes, followed by Bacteroidetes and Proteobacteria, which are characteristic of herbivore gut microbiota. Some microbiota variation existed between turtles and among gastrointestinal sections. The 16S sequence analysis provided a better representation of the total gastrointestinal bacterial community, much of which cannot be cultured using traditional microbial techniques. These metagenomic analyses serve as a foundation for a better understanding of the microbiome of green turtles in the Hawaiian Islands and elsewhere.
format Online
Article
Text
id pubmed-7160772
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-71607722020-04-20 Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas) McDermid, Karla J Kittle, Ronald P Veillet, Anne Plouviez, Sophie Muehlstein, Lisa Balazs, George H Evol Bioinform Online Original Research Green turtles (Chelonia mydas) have a hindgut fermentation digestive tract, which uses cellulolytic microbes to break down plant matter in the cecum and proximal colon. Previous studies on bacterial communities of green turtles have not identified in situ hindgut microbiota, and never before in Hawaiian green turtles, which comprise an isolated metapopulation. Fresh samples using sterile swabs were taken from five locations along the gastrointestinal tracts of eight green turtles that had required euthanization. Bacteria were cultured, aerobically and anaerobically, on nutrient agar and four differential and selective media. Samples at three sections along the gastrointestinal tracts of two green turtles were analyzed using 16S metagenomics on an Ion Torrent Personal Genome Machine. More than half of the 4 532 104 sequences belonged to the phylum Firmicutes, followed by Bacteroidetes and Proteobacteria, which are characteristic of herbivore gut microbiota. Some microbiota variation existed between turtles and among gastrointestinal sections. The 16S sequence analysis provided a better representation of the total gastrointestinal bacterial community, much of which cannot be cultured using traditional microbial techniques. These metagenomic analyses serve as a foundation for a better understanding of the microbiome of green turtles in the Hawaiian Islands and elsewhere. SAGE Publications 2020-04-15 /pmc/articles/PMC7160772/ /pubmed/32313421 http://dx.doi.org/10.1177/1176934320914603 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
McDermid, Karla J
Kittle, Ronald P
Veillet, Anne
Plouviez, Sophie
Muehlstein, Lisa
Balazs, George H
Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas)
title Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas)
title_full Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas)
title_fullStr Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas)
title_full_unstemmed Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas)
title_short Identification of Gastrointestinal Microbiota in Hawaiian Green Turtles (Chelonia mydas)
title_sort identification of gastrointestinal microbiota in hawaiian green turtles (chelonia mydas)
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160772/
https://www.ncbi.nlm.nih.gov/pubmed/32313421
http://dx.doi.org/10.1177/1176934320914603
work_keys_str_mv AT mcdermidkarlaj identificationofgastrointestinalmicrobiotainhawaiiangreenturtlescheloniamydas
AT kittleronaldp identificationofgastrointestinalmicrobiotainhawaiiangreenturtlescheloniamydas
AT veilletanne identificationofgastrointestinalmicrobiotainhawaiiangreenturtlescheloniamydas
AT plouviezsophie identificationofgastrointestinalmicrobiotainhawaiiangreenturtlescheloniamydas
AT muehlsteinlisa identificationofgastrointestinalmicrobiotainhawaiiangreenturtlescheloniamydas
AT balazsgeorgeh identificationofgastrointestinalmicrobiotainhawaiiangreenturtlescheloniamydas