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Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production

BACKGROUND: Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implemen...

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Autores principales: Stylianou, Eleni, Pateraki, Chrysanthi, Ladakis, Dimitrios, Cruz-Fernández, María, Latorre-Sánchez, Marcos, Coll, Caterina, Koutinas, Apostolis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160979/
https://www.ncbi.nlm.nih.gov/pubmed/32322302
http://dx.doi.org/10.1186/s13068-020-01708-w
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author Stylianou, Eleni
Pateraki, Chrysanthi
Ladakis, Dimitrios
Cruz-Fernández, María
Latorre-Sánchez, Marcos
Coll, Caterina
Koutinas, Apostolis
author_facet Stylianou, Eleni
Pateraki, Chrysanthi
Ladakis, Dimitrios
Cruz-Fernández, María
Latorre-Sánchez, Marcos
Coll, Caterina
Koutinas, Apostolis
author_sort Stylianou, Eleni
collection PubMed
description BACKGROUND: Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation. RESULTS: Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. A tailor-made enzymatic cocktail was used for OFMSW hydrolysate production containing up to 107.3 g/L carbon sources and up to 638.7 mg/L free amino nitrogen. The bacterial strains Actinobacillus succinogenes and Basfia succiniciproducens were evaluated for succinic acid production with the latter strain being less efficient due to high lactic acid production. Batch A. succinogenes cultures supplemented with 5 g/L yeast extract and 5 g/L MgCO(3) reached 29.4 g/L succinic acid with productivity of 0.89 g/L/h and yield of 0.56 g/g. Continuous cultures at dilution rate of 0.06 h(−1) reached 21.2 g/L succinic acid with yield of 0.47 g/g and productivity of 1.27 g/L/h. Downstream separation and purification of succinic acid was achieved by centrifugation, treatment with activated carbon, acidification with cation exchange resins, evaporation and drying, reaching more than 99% purity. Preliminary techno-economic evaluation has been employed to evaluate the profitability potential of bio-based succinic acid production. CONCLUSIONS: The use of OFMSW hydrolysate in continuous cultures could lead to a minimum selling price of 2.5 $/kg at annual production capacity of 40,000 t succinic acid and OFMSW hydrolysate production cost of 25 $/t sugars.
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spelling pubmed-71609792020-04-22 Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production Stylianou, Eleni Pateraki, Chrysanthi Ladakis, Dimitrios Cruz-Fernández, María Latorre-Sánchez, Marcos Coll, Caterina Koutinas, Apostolis Biotechnol Biofuels Research BACKGROUND: Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation. RESULTS: Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. A tailor-made enzymatic cocktail was used for OFMSW hydrolysate production containing up to 107.3 g/L carbon sources and up to 638.7 mg/L free amino nitrogen. The bacterial strains Actinobacillus succinogenes and Basfia succiniciproducens were evaluated for succinic acid production with the latter strain being less efficient due to high lactic acid production. Batch A. succinogenes cultures supplemented with 5 g/L yeast extract and 5 g/L MgCO(3) reached 29.4 g/L succinic acid with productivity of 0.89 g/L/h and yield of 0.56 g/g. Continuous cultures at dilution rate of 0.06 h(−1) reached 21.2 g/L succinic acid with yield of 0.47 g/g and productivity of 1.27 g/L/h. Downstream separation and purification of succinic acid was achieved by centrifugation, treatment with activated carbon, acidification with cation exchange resins, evaporation and drying, reaching more than 99% purity. Preliminary techno-economic evaluation has been employed to evaluate the profitability potential of bio-based succinic acid production. CONCLUSIONS: The use of OFMSW hydrolysate in continuous cultures could lead to a minimum selling price of 2.5 $/kg at annual production capacity of 40,000 t succinic acid and OFMSW hydrolysate production cost of 25 $/t sugars. BioMed Central 2020-04-15 /pmc/articles/PMC7160979/ /pubmed/32322302 http://dx.doi.org/10.1186/s13068-020-01708-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Stylianou, Eleni
Pateraki, Chrysanthi
Ladakis, Dimitrios
Cruz-Fernández, María
Latorre-Sánchez, Marcos
Coll, Caterina
Koutinas, Apostolis
Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production
title Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production
title_full Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production
title_fullStr Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production
title_full_unstemmed Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production
title_short Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production
title_sort evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160979/
https://www.ncbi.nlm.nih.gov/pubmed/32322302
http://dx.doi.org/10.1186/s13068-020-01708-w
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