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High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids

BACKGROUND: To investigate the effect of transabdominal hysterectomy on the diversity of the intestinal flora in patients with uterine fibroids. Patients with uterine fibroids were selected from September 2018 to December 2018, in the Department of Obstetrics and Gynecology, Shengjing Hospital of Ch...

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Autores principales: Wang, Wantong, Li, Yibing, Wu, Qijun, Pan, Xin, He, Xinhui, Ma, Xiaoxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161020/
https://www.ncbi.nlm.nih.gov/pubmed/32299359
http://dx.doi.org/10.1186/s12866-020-01779-7
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author Wang, Wantong
Li, Yibing
Wu, Qijun
Pan, Xin
He, Xinhui
Ma, Xiaoxin
author_facet Wang, Wantong
Li, Yibing
Wu, Qijun
Pan, Xin
He, Xinhui
Ma, Xiaoxin
author_sort Wang, Wantong
collection PubMed
description BACKGROUND: To investigate the effect of transabdominal hysterectomy on the diversity of the intestinal flora in patients with uterine fibroids. Patients with uterine fibroids were selected from September 2018 to December 2018, in the Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, and stool specimens were collected from patients before and after surgery. RESULTS: High-throughput sequencing of the 16S rRNA gene was used to detect the changes in microbial community structure and diversity, and the effects of total hysterectomy on the intestinal flora were further analyzed. Estrogen levels decreased after trans-abdominal hysterectomy. High-throughput sequencing showed that after abdominal hysterectomy, the abundance and diversity of the intestinal flora decreased. The abundance changes were mainly due to Proteobacteria, where their abundance increased. CONCLUSIONS: Trans-abdominal hysterectomy changes the intestinal flora of the body by lowering the level of estrogen in the body, which reduces the diversity and abundance of the intestinal flora.
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spelling pubmed-71610202020-04-22 High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids Wang, Wantong Li, Yibing Wu, Qijun Pan, Xin He, Xinhui Ma, Xiaoxin BMC Microbiol Research Article BACKGROUND: To investigate the effect of transabdominal hysterectomy on the diversity of the intestinal flora in patients with uterine fibroids. Patients with uterine fibroids were selected from September 2018 to December 2018, in the Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, and stool specimens were collected from patients before and after surgery. RESULTS: High-throughput sequencing of the 16S rRNA gene was used to detect the changes in microbial community structure and diversity, and the effects of total hysterectomy on the intestinal flora were further analyzed. Estrogen levels decreased after trans-abdominal hysterectomy. High-throughput sequencing showed that after abdominal hysterectomy, the abundance and diversity of the intestinal flora decreased. The abundance changes were mainly due to Proteobacteria, where their abundance increased. CONCLUSIONS: Trans-abdominal hysterectomy changes the intestinal flora of the body by lowering the level of estrogen in the body, which reduces the diversity and abundance of the intestinal flora. BioMed Central 2020-04-15 /pmc/articles/PMC7161020/ /pubmed/32299359 http://dx.doi.org/10.1186/s12866-020-01779-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wang, Wantong
Li, Yibing
Wu, Qijun
Pan, Xin
He, Xinhui
Ma, Xiaoxin
High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids
title High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids
title_full High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids
title_fullStr High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids
title_full_unstemmed High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids
title_short High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids
title_sort high-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161020/
https://www.ncbi.nlm.nih.gov/pubmed/32299359
http://dx.doi.org/10.1186/s12866-020-01779-7
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