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Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop

[Image: see text] In India, the major crop is wheat. Its production is severely hampered by seed-borne diseases such as smut and bunt which are responsible for the reduction of crop yield with poor grain quality. In the current study, an attempt was made to prepare a photoluminescence (PL)-based imm...

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Autores principales: Mishra, Manjita, Singh, Shailendra Kumar, Bhardwaj, Abhishek, Kumar, Lokendra, Singh, Manoj Kumar, Sundaram, Shanthy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161024/
https://www.ncbi.nlm.nih.gov/pubmed/32309735
http://dx.doi.org/10.1021/acsomega.0c00551
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author Mishra, Manjita
Singh, Shailendra Kumar
Bhardwaj, Abhishek
Kumar, Lokendra
Singh, Manoj Kumar
Sundaram, Shanthy
author_facet Mishra, Manjita
Singh, Shailendra Kumar
Bhardwaj, Abhishek
Kumar, Lokendra
Singh, Manoj Kumar
Sundaram, Shanthy
author_sort Mishra, Manjita
collection PubMed
description [Image: see text] In India, the major crop is wheat. Its production is severely hampered by seed-borne diseases such as smut and bunt which are responsible for the reduction of crop yield with poor grain quality. In the current study, an attempt was made to prepare a photoluminescence (PL)-based immunosensor for early detection of Karnal bunt (KB) disease. The KB disease-causing pathogen Tilletia indica was detected using functionalized diatom frustules as a sensing platform. The teliospore-covered platform, on exposure to light, showed enhanced intensity of PL in comparison to control. This response was directly proportional to the concentration of spores. For the development of a stable frustule-based immunosensor platform, gluteraldehyde was added for the covalent immobilization of the T. indica antibody onto amine-functionalized diatom substrates. Frustules of diatom consisting of a nanoporous three-dimensional biogenic silica material exhibit a unique property of emitting strong, visible blue PL under ultraviolet (UV) excitation. PL studies were done to reveal the specificity and binding of the conjugated diatom platform that will distinguish between the T. indica (complementary) and A. niger (noncomplementary) antigens. Four times better intensity of PL was observed against the complementary one in comparison to a noncomplementary setup (control). The immunocomplex frustule-based platform serves as a suitable sensor platform for early detection of KB.
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spelling pubmed-71610242020-04-17 Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop Mishra, Manjita Singh, Shailendra Kumar Bhardwaj, Abhishek Kumar, Lokendra Singh, Manoj Kumar Sundaram, Shanthy ACS Omega [Image: see text] In India, the major crop is wheat. Its production is severely hampered by seed-borne diseases such as smut and bunt which are responsible for the reduction of crop yield with poor grain quality. In the current study, an attempt was made to prepare a photoluminescence (PL)-based immunosensor for early detection of Karnal bunt (KB) disease. The KB disease-causing pathogen Tilletia indica was detected using functionalized diatom frustules as a sensing platform. The teliospore-covered platform, on exposure to light, showed enhanced intensity of PL in comparison to control. This response was directly proportional to the concentration of spores. For the development of a stable frustule-based immunosensor platform, gluteraldehyde was added for the covalent immobilization of the T. indica antibody onto amine-functionalized diatom substrates. Frustules of diatom consisting of a nanoporous three-dimensional biogenic silica material exhibit a unique property of emitting strong, visible blue PL under ultraviolet (UV) excitation. PL studies were done to reveal the specificity and binding of the conjugated diatom platform that will distinguish between the T. indica (complementary) and A. niger (noncomplementary) antigens. Four times better intensity of PL was observed against the complementary one in comparison to a noncomplementary setup (control). The immunocomplex frustule-based platform serves as a suitable sensor platform for early detection of KB. American Chemical Society 2020-04-02 /pmc/articles/PMC7161024/ /pubmed/32309735 http://dx.doi.org/10.1021/acsomega.0c00551 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Mishra, Manjita
Singh, Shailendra Kumar
Bhardwaj, Abhishek
Kumar, Lokendra
Singh, Manoj Kumar
Sundaram, Shanthy
Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop
title Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop
title_full Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop
title_fullStr Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop
title_full_unstemmed Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop
title_short Development of a Diatom-Based Photoluminescent Immunosensor for the Early Detection of Karnal Bunt Disease of Wheat Crop
title_sort development of a diatom-based photoluminescent immunosensor for the early detection of karnal bunt disease of wheat crop
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161024/
https://www.ncbi.nlm.nih.gov/pubmed/32309735
http://dx.doi.org/10.1021/acsomega.0c00551
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