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Susceptible gene polymorphism in patients with three-vessel coronary artery disease

BACKGROUND: Data of susceptible gene polymorphisms related to progression of coronary atherosclerosis in patients with three-vessel disease (TVD) is limited in China. This case-control study aimed to analyze the differences of variant carrier frequencies between cases and controls, and to explain th...

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Autores principales: Liu, Ru, Song, Lei, Jiang, Lin, Tang, Xiaofang, Xu, Lianjun, Gao, Zhan, Zhao, Xueyan, Xu, Jingjing, Gao, Runlin, Yuan, Jinqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161109/
https://www.ncbi.nlm.nih.gov/pubmed/32293292
http://dx.doi.org/10.1186/s12872-020-01449-6
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author Liu, Ru
Song, Lei
Jiang, Lin
Tang, Xiaofang
Xu, Lianjun
Gao, Zhan
Zhao, Xueyan
Xu, Jingjing
Gao, Runlin
Yuan, Jinqing
author_facet Liu, Ru
Song, Lei
Jiang, Lin
Tang, Xiaofang
Xu, Lianjun
Gao, Zhan
Zhao, Xueyan
Xu, Jingjing
Gao, Runlin
Yuan, Jinqing
author_sort Liu, Ru
collection PubMed
description BACKGROUND: Data of susceptible gene polymorphisms related to progression of coronary atherosclerosis in patients with three-vessel disease (TVD) is limited in China. This case-control study aimed to analyze the differences of variant carrier frequencies between cases and controls, and to explain the possible genetic effects on the progression of TVD. METHODS: A total of 8943 TVD patients were consecutively enrolled. Major adverse cardiac and cerebrovascular events (MACCE) included all-cause death, acute myocardial infarction, repeat revascularization, readmission and stroke. Patients with 1-year MACCE in this cohort were selected as MACCE group. Blood samples from MACCE group and non-CAD control groups were collected, and a deoxyribonucleic acid library was created. A total of 34 tag or hot single nucleotide polymorphisms (SNPs) in six genes including CDKN2B-AS1, ADAMTS7, ABO, ADAMTS13, IL-18, and PECAM1 were analyzed by a SNPscan™ multi-genotyping kit. Carrier frequencies of each SNP were compared between the two groups using dominant, recessive and codominant allele model, respectively. Multivariate logistic regression model was established. RESULTS: Variant allele frequencies of rs10757274, rs1333042, rs1333049, rs4977574, rs9632884, rs1063192 and rs3217986 on CDKN2B-AS1 gene showed significant differences between the two groups in at least one allele model. Variant allele frequency of rs3217986 was not statistically significant after adjusting for the false discovery rate using Benjamini-Hochberg procedure (Q > 0.05). Variant allele frequencies of rs1333049, rs10757274, rs4977574 on CDKN2B-AS1 gene were significantly higher in MACCE group in all dominant, recessive and codominant models. Rs1055432 on ADAMTS13 and rs8176694 on ABO gene showed threshold significance between the two groups. After multivariable adjustment, G mutant homozygous rs9632884 (GG vs. GC + CC) (OR: 0.24; 95% CI: 0.09–0.65; P = 0.005) on CDKN2B-AS1 gene were independent protective factor of MACCE in recessive model. CONCLUSIONS: In patients with TVD in China, variant alleles on CDKN2B-AS1 gene may form part of the genetic basis of coronary atherosclerosis progression, promoting or suppressing ischemic events.
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spelling pubmed-71611092020-04-22 Susceptible gene polymorphism in patients with three-vessel coronary artery disease Liu, Ru Song, Lei Jiang, Lin Tang, Xiaofang Xu, Lianjun Gao, Zhan Zhao, Xueyan Xu, Jingjing Gao, Runlin Yuan, Jinqing BMC Cardiovasc Disord Research Article BACKGROUND: Data of susceptible gene polymorphisms related to progression of coronary atherosclerosis in patients with three-vessel disease (TVD) is limited in China. This case-control study aimed to analyze the differences of variant carrier frequencies between cases and controls, and to explain the possible genetic effects on the progression of TVD. METHODS: A total of 8943 TVD patients were consecutively enrolled. Major adverse cardiac and cerebrovascular events (MACCE) included all-cause death, acute myocardial infarction, repeat revascularization, readmission and stroke. Patients with 1-year MACCE in this cohort were selected as MACCE group. Blood samples from MACCE group and non-CAD control groups were collected, and a deoxyribonucleic acid library was created. A total of 34 tag or hot single nucleotide polymorphisms (SNPs) in six genes including CDKN2B-AS1, ADAMTS7, ABO, ADAMTS13, IL-18, and PECAM1 were analyzed by a SNPscan™ multi-genotyping kit. Carrier frequencies of each SNP were compared between the two groups using dominant, recessive and codominant allele model, respectively. Multivariate logistic regression model was established. RESULTS: Variant allele frequencies of rs10757274, rs1333042, rs1333049, rs4977574, rs9632884, rs1063192 and rs3217986 on CDKN2B-AS1 gene showed significant differences between the two groups in at least one allele model. Variant allele frequency of rs3217986 was not statistically significant after adjusting for the false discovery rate using Benjamini-Hochberg procedure (Q > 0.05). Variant allele frequencies of rs1333049, rs10757274, rs4977574 on CDKN2B-AS1 gene were significantly higher in MACCE group in all dominant, recessive and codominant models. Rs1055432 on ADAMTS13 and rs8176694 on ABO gene showed threshold significance between the two groups. After multivariable adjustment, G mutant homozygous rs9632884 (GG vs. GC + CC) (OR: 0.24; 95% CI: 0.09–0.65; P = 0.005) on CDKN2B-AS1 gene were independent protective factor of MACCE in recessive model. CONCLUSIONS: In patients with TVD in China, variant alleles on CDKN2B-AS1 gene may form part of the genetic basis of coronary atherosclerosis progression, promoting or suppressing ischemic events. BioMed Central 2020-04-15 /pmc/articles/PMC7161109/ /pubmed/32293292 http://dx.doi.org/10.1186/s12872-020-01449-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Liu, Ru
Song, Lei
Jiang, Lin
Tang, Xiaofang
Xu, Lianjun
Gao, Zhan
Zhao, Xueyan
Xu, Jingjing
Gao, Runlin
Yuan, Jinqing
Susceptible gene polymorphism in patients with three-vessel coronary artery disease
title Susceptible gene polymorphism in patients with three-vessel coronary artery disease
title_full Susceptible gene polymorphism in patients with three-vessel coronary artery disease
title_fullStr Susceptible gene polymorphism in patients with three-vessel coronary artery disease
title_full_unstemmed Susceptible gene polymorphism in patients with three-vessel coronary artery disease
title_short Susceptible gene polymorphism in patients with three-vessel coronary artery disease
title_sort susceptible gene polymorphism in patients with three-vessel coronary artery disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161109/
https://www.ncbi.nlm.nih.gov/pubmed/32293292
http://dx.doi.org/10.1186/s12872-020-01449-6
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