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Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections
BACKGROUND: Because of their similar modes of transmission, the simultaneous infection of viral hepatitis and human immunodeficiency virus are increasingly seen as a big problem related to human health. AIMS: To determine the drug mutations in hepatitis B virus and/or hepatitis C virus co-infected h...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Galenos Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161615/ https://www.ncbi.nlm.nih.gov/pubmed/32106666 http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.5.89 |
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author | Sayan, Murat Özgüler, Müge Sarıgül Yıldırım, Figen Yıldırmak, Taner Gündüz, Alper Dokuzoğuz, Başak Çelen, Mustafa Kemal İnan, Dilara Heper, Yasemin Ersöz, Gülden Karaoğlan, İlkay Ceran, Nurgül Deveci, Aydın Öztürk, Servet Sayın Kutlu, Selda Özkan Özdemir, Hülya Akbulut, Ayhan Yazıcı, Saadet Şener, Alper Çağatay, Atahan Ünal, Serhat |
author_facet | Sayan, Murat Özgüler, Müge Sarıgül Yıldırım, Figen Yıldırmak, Taner Gündüz, Alper Dokuzoğuz, Başak Çelen, Mustafa Kemal İnan, Dilara Heper, Yasemin Ersöz, Gülden Karaoğlan, İlkay Ceran, Nurgül Deveci, Aydın Öztürk, Servet Sayın Kutlu, Selda Özkan Özdemir, Hülya Akbulut, Ayhan Yazıcı, Saadet Şener, Alper Çağatay, Atahan Ünal, Serhat |
author_sort | Sayan, Murat |
collection | PubMed |
description | BACKGROUND: Because of their similar modes of transmission, the simultaneous infection of viral hepatitis and human immunodeficiency virus are increasingly seen as a big problem related to human health. AIMS: To determine the drug mutations in hepatitis B virus and/or hepatitis C virus co-infected human immunodeficiency virus-1 patients in Turkey. STUDY DESIGN: Retrospective cross-sectional study. METHODS: The present study was conducted between 2010 and 2017. HBsAg, anti-hepatitis C virus, and anti-human immunodeficiency virus were tested with ELISA. All anti-human immunodeficiency virus positive results by ELISA were verified for anti-human immunodeficiency virus positivity by a Western blot test, and Anti-human immunodeficiency virus positive patients with HBsAg and/or anti-hepatitis C virus positivity were included in the study. Subtyping and genotypic resistance analyses were performed by population sequencing of the viral protease and reverse transcriptase regions of the human immunodeficiency virus-1 pol gene. RESULTS: We detected 3896 human immunodeficiency virus-1 positive patients whose sera were sent from numerous hospitals across the country to our polymerase chain reaction unit for detection of drug resistance mutations and whose molecular laboratory tests were completed. Viral hepatitis co-infections were detected in 4.3% (n=170) of patients. Hepatitis B virus and hepatitis C virus co-infection were observed in 3.2% and 0.5% of all human immunodeficiency virus-1 infected patients, respectively. The major human immunodeficiency virus-1 subtype detected was group M, subtype B (62.9%). However, 13.5% of drug resistance mutation motifs were found in human immunodeficiency virus-1 genomes of patients included in the study. CONCLUSION: Due to similar transmission routes, HIV1 patients are at risk of hepatitis B and C virus co-infection. However, antiretroviral drug resistance mutation model is similar to patients with hepatitis negative. |
format | Online Article Text |
id | pubmed-7161615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Galenos Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-71616152020-05-01 Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections Sayan, Murat Özgüler, Müge Sarıgül Yıldırım, Figen Yıldırmak, Taner Gündüz, Alper Dokuzoğuz, Başak Çelen, Mustafa Kemal İnan, Dilara Heper, Yasemin Ersöz, Gülden Karaoğlan, İlkay Ceran, Nurgül Deveci, Aydın Öztürk, Servet Sayın Kutlu, Selda Özkan Özdemir, Hülya Akbulut, Ayhan Yazıcı, Saadet Şener, Alper Çağatay, Atahan Ünal, Serhat Balkan Med J Original Article BACKGROUND: Because of their similar modes of transmission, the simultaneous infection of viral hepatitis and human immunodeficiency virus are increasingly seen as a big problem related to human health. AIMS: To determine the drug mutations in hepatitis B virus and/or hepatitis C virus co-infected human immunodeficiency virus-1 patients in Turkey. STUDY DESIGN: Retrospective cross-sectional study. METHODS: The present study was conducted between 2010 and 2017. HBsAg, anti-hepatitis C virus, and anti-human immunodeficiency virus were tested with ELISA. All anti-human immunodeficiency virus positive results by ELISA were verified for anti-human immunodeficiency virus positivity by a Western blot test, and Anti-human immunodeficiency virus positive patients with HBsAg and/or anti-hepatitis C virus positivity were included in the study. Subtyping and genotypic resistance analyses were performed by population sequencing of the viral protease and reverse transcriptase regions of the human immunodeficiency virus-1 pol gene. RESULTS: We detected 3896 human immunodeficiency virus-1 positive patients whose sera were sent from numerous hospitals across the country to our polymerase chain reaction unit for detection of drug resistance mutations and whose molecular laboratory tests were completed. Viral hepatitis co-infections were detected in 4.3% (n=170) of patients. Hepatitis B virus and hepatitis C virus co-infection were observed in 3.2% and 0.5% of all human immunodeficiency virus-1 infected patients, respectively. The major human immunodeficiency virus-1 subtype detected was group M, subtype B (62.9%). However, 13.5% of drug resistance mutation motifs were found in human immunodeficiency virus-1 genomes of patients included in the study. CONCLUSION: Due to similar transmission routes, HIV1 patients are at risk of hepatitis B and C virus co-infection. However, antiretroviral drug resistance mutation model is similar to patients with hepatitis negative. Galenos Publishing 2020-05 2020-04-10 /pmc/articles/PMC7161615/ /pubmed/32106666 http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.5.89 Text en ©Copyright 2020 by Trakya University Faculty of Medicine http://creativecommons.org/licenses/by/2.5/ The Balkan Medical Journal published by Galenos Publishing House. |
spellingShingle | Original Article Sayan, Murat Özgüler, Müge Sarıgül Yıldırım, Figen Yıldırmak, Taner Gündüz, Alper Dokuzoğuz, Başak Çelen, Mustafa Kemal İnan, Dilara Heper, Yasemin Ersöz, Gülden Karaoğlan, İlkay Ceran, Nurgül Deveci, Aydın Öztürk, Servet Sayın Kutlu, Selda Özkan Özdemir, Hülya Akbulut, Ayhan Yazıcı, Saadet Şener, Alper Çağatay, Atahan Ünal, Serhat Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections |
title | Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections |
title_full | Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections |
title_fullStr | Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections |
title_full_unstemmed | Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections |
title_short | Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections |
title_sort | molecular identification of hiv-1 in the presence of hepatitis b virus and hepatitis c virus co-infections |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161615/ https://www.ncbi.nlm.nih.gov/pubmed/32106666 http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.5.89 |
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