Cargando…

Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections

BACKGROUND: Because of their similar modes of transmission, the simultaneous infection of viral hepatitis and human immunodeficiency virus are increasingly seen as a big problem related to human health. AIMS: To determine the drug mutations in hepatitis B virus and/or hepatitis C virus co-infected h...

Descripción completa

Detalles Bibliográficos
Autores principales: Sayan, Murat, Özgüler, Müge, Sarıgül Yıldırım, Figen, Yıldırmak, Taner, Gündüz, Alper, Dokuzoğuz, Başak, Çelen, Mustafa Kemal, İnan, Dilara, Heper, Yasemin, Ersöz, Gülden, Karaoğlan, İlkay, Ceran, Nurgül, Deveci, Aydın, Öztürk, Servet, Sayın Kutlu, Selda, Özkan Özdemir, Hülya, Akbulut, Ayhan, Yazıcı, Saadet, Şener, Alper, Çağatay, Atahan, Ünal, Serhat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Galenos Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161615/
https://www.ncbi.nlm.nih.gov/pubmed/32106666
http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.5.89
_version_ 1783522980539662336
author Sayan, Murat
Özgüler, Müge
Sarıgül Yıldırım, Figen
Yıldırmak, Taner
Gündüz, Alper
Dokuzoğuz, Başak
Çelen, Mustafa Kemal
İnan, Dilara
Heper, Yasemin
Ersöz, Gülden
Karaoğlan, İlkay
Ceran, Nurgül
Deveci, Aydın
Öztürk, Servet
Sayın Kutlu, Selda
Özkan Özdemir, Hülya
Akbulut, Ayhan
Yazıcı, Saadet
Şener, Alper
Çağatay, Atahan
Ünal, Serhat
author_facet Sayan, Murat
Özgüler, Müge
Sarıgül Yıldırım, Figen
Yıldırmak, Taner
Gündüz, Alper
Dokuzoğuz, Başak
Çelen, Mustafa Kemal
İnan, Dilara
Heper, Yasemin
Ersöz, Gülden
Karaoğlan, İlkay
Ceran, Nurgül
Deveci, Aydın
Öztürk, Servet
Sayın Kutlu, Selda
Özkan Özdemir, Hülya
Akbulut, Ayhan
Yazıcı, Saadet
Şener, Alper
Çağatay, Atahan
Ünal, Serhat
author_sort Sayan, Murat
collection PubMed
description BACKGROUND: Because of their similar modes of transmission, the simultaneous infection of viral hepatitis and human immunodeficiency virus are increasingly seen as a big problem related to human health. AIMS: To determine the drug mutations in hepatitis B virus and/or hepatitis C virus co-infected human immunodeficiency virus-1 patients in Turkey. STUDY DESIGN: Retrospective cross-sectional study. METHODS: The present study was conducted between 2010 and 2017. HBsAg, anti-hepatitis C virus, and anti-human immunodeficiency virus were tested with ELISA. All anti-human immunodeficiency virus positive results by ELISA were verified for anti-human immunodeficiency virus positivity by a Western blot test, and Anti-human immunodeficiency virus positive patients with HBsAg and/or anti-hepatitis C virus positivity were included in the study. Subtyping and genotypic resistance analyses were performed by population sequencing of the viral protease and reverse transcriptase regions of the human immunodeficiency virus-1 pol gene. RESULTS: We detected 3896 human immunodeficiency virus-1 positive patients whose sera were sent from numerous hospitals across the country to our polymerase chain reaction unit for detection of drug resistance mutations and whose molecular laboratory tests were completed. Viral hepatitis co-infections were detected in 4.3% (n=170) of patients. Hepatitis B virus and hepatitis C virus co-infection were observed in 3.2% and 0.5% of all human immunodeficiency virus-1 infected patients, respectively. The major human immunodeficiency virus-1 subtype detected was group M, subtype B (62.9%). However, 13.5% of drug resistance mutation motifs were found in human immunodeficiency virus-1 genomes of patients included in the study. CONCLUSION: Due to similar transmission routes, HIV1 patients are at risk of hepatitis B and C virus co-infection. However, antiretroviral drug resistance mutation model is similar to patients with hepatitis negative.
format Online
Article
Text
id pubmed-7161615
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Galenos Publishing
record_format MEDLINE/PubMed
spelling pubmed-71616152020-05-01 Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections Sayan, Murat Özgüler, Müge Sarıgül Yıldırım, Figen Yıldırmak, Taner Gündüz, Alper Dokuzoğuz, Başak Çelen, Mustafa Kemal İnan, Dilara Heper, Yasemin Ersöz, Gülden Karaoğlan, İlkay Ceran, Nurgül Deveci, Aydın Öztürk, Servet Sayın Kutlu, Selda Özkan Özdemir, Hülya Akbulut, Ayhan Yazıcı, Saadet Şener, Alper Çağatay, Atahan Ünal, Serhat Balkan Med J Original Article BACKGROUND: Because of their similar modes of transmission, the simultaneous infection of viral hepatitis and human immunodeficiency virus are increasingly seen as a big problem related to human health. AIMS: To determine the drug mutations in hepatitis B virus and/or hepatitis C virus co-infected human immunodeficiency virus-1 patients in Turkey. STUDY DESIGN: Retrospective cross-sectional study. METHODS: The present study was conducted between 2010 and 2017. HBsAg, anti-hepatitis C virus, and anti-human immunodeficiency virus were tested with ELISA. All anti-human immunodeficiency virus positive results by ELISA were verified for anti-human immunodeficiency virus positivity by a Western blot test, and Anti-human immunodeficiency virus positive patients with HBsAg and/or anti-hepatitis C virus positivity were included in the study. Subtyping and genotypic resistance analyses were performed by population sequencing of the viral protease and reverse transcriptase regions of the human immunodeficiency virus-1 pol gene. RESULTS: We detected 3896 human immunodeficiency virus-1 positive patients whose sera were sent from numerous hospitals across the country to our polymerase chain reaction unit for detection of drug resistance mutations and whose molecular laboratory tests were completed. Viral hepatitis co-infections were detected in 4.3% (n=170) of patients. Hepatitis B virus and hepatitis C virus co-infection were observed in 3.2% and 0.5% of all human immunodeficiency virus-1 infected patients, respectively. The major human immunodeficiency virus-1 subtype detected was group M, subtype B (62.9%). However, 13.5% of drug resistance mutation motifs were found in human immunodeficiency virus-1 genomes of patients included in the study. CONCLUSION: Due to similar transmission routes, HIV1 patients are at risk of hepatitis B and C virus co-infection. However, antiretroviral drug resistance mutation model is similar to patients with hepatitis negative. Galenos Publishing 2020-05 2020-04-10 /pmc/articles/PMC7161615/ /pubmed/32106666 http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.5.89 Text en ©Copyright 2020 by Trakya University Faculty of Medicine http://creativecommons.org/licenses/by/2.5/ The Balkan Medical Journal published by Galenos Publishing House.
spellingShingle Original Article
Sayan, Murat
Özgüler, Müge
Sarıgül Yıldırım, Figen
Yıldırmak, Taner
Gündüz, Alper
Dokuzoğuz, Başak
Çelen, Mustafa Kemal
İnan, Dilara
Heper, Yasemin
Ersöz, Gülden
Karaoğlan, İlkay
Ceran, Nurgül
Deveci, Aydın
Öztürk, Servet
Sayın Kutlu, Selda
Özkan Özdemir, Hülya
Akbulut, Ayhan
Yazıcı, Saadet
Şener, Alper
Çağatay, Atahan
Ünal, Serhat
Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections
title Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections
title_full Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections
title_fullStr Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections
title_full_unstemmed Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections
title_short Molecular Identification of HIV-1 in the Presence of Hepatitis B Virus and Hepatitis C Virus Co-infections
title_sort molecular identification of hiv-1 in the presence of hepatitis b virus and hepatitis c virus co-infections
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161615/
https://www.ncbi.nlm.nih.gov/pubmed/32106666
http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.5.89
work_keys_str_mv AT sayanmurat molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT ozgulermuge molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT sarıgulyıldırımfigen molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT yıldırmaktaner molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT gunduzalper molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT dokuzoguzbasak molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT celenmustafakemal molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT inandilara molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT heperyasemin molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT ersozgulden molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT karaoglanilkay molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT cerannurgul molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT deveciaydın molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT ozturkservet molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT sayınkutluselda molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT ozkanozdemirhulya molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT akbulutayhan molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT yazıcısaadet molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT seneralper molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT cagatayatahan molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections
AT unalserhat molecularidentificationofhiv1inthepresenceofhepatitisbvirusandhepatitiscviruscoinfections