Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese

The WST-1 assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based assays can, however, be affected by the interference of tested chemicals, including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 assay. E...

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Detalles Bibliográficos
Autores principales: Scarcello, Eleonora, Lambremont, Alexia, Vanbever, Rita, Jacques, Pascal J., Lison, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161962/
https://www.ncbi.nlm.nih.gov/pubmed/32298350
http://dx.doi.org/10.1371/journal.pone.0231634
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author Scarcello, Eleonora
Lambremont, Alexia
Vanbever, Rita
Jacques, Pascal J.
Lison, Dominique
author_facet Scarcello, Eleonora
Lambremont, Alexia
Vanbever, Rita
Jacques, Pascal J.
Lison, Dominique
author_sort Scarcello, Eleonora
collection PubMed
description The WST-1 assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based assays can, however, be affected by the interference of tested chemicals, including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 assay. Endothelial cells exposed to Mn particles (Mn alone or Fe-Mn alloy from 50 to 1600 μg/ml) were severely damaged according to the WST-1 assay, but not the ATP content assay. Subsequent experiments revealed that Mn particles interfere with the reduction of the tetrazolium salt to formazan. Therefore, the WST-1 assay is not suitable to evaluate the in vitro cytotoxicity of Mn-containing materials, and luminescence-based assays such as CellTiter-Glo(®) appear more appropriate.
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spelling pubmed-71619622020-04-21 Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese Scarcello, Eleonora Lambremont, Alexia Vanbever, Rita Jacques, Pascal J. Lison, Dominique PLoS One Research Article The WST-1 assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based assays can, however, be affected by the interference of tested chemicals, including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 assay. Endothelial cells exposed to Mn particles (Mn alone or Fe-Mn alloy from 50 to 1600 μg/ml) were severely damaged according to the WST-1 assay, but not the ATP content assay. Subsequent experiments revealed that Mn particles interfere with the reduction of the tetrazolium salt to formazan. Therefore, the WST-1 assay is not suitable to evaluate the in vitro cytotoxicity of Mn-containing materials, and luminescence-based assays such as CellTiter-Glo(®) appear more appropriate. Public Library of Science 2020-04-16 /pmc/articles/PMC7161962/ /pubmed/32298350 http://dx.doi.org/10.1371/journal.pone.0231634 Text en © 2020 Scarcello et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Scarcello, Eleonora
Lambremont, Alexia
Vanbever, Rita
Jacques, Pascal J.
Lison, Dominique
Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese
title Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese
title_full Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese
title_fullStr Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese
title_full_unstemmed Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese
title_short Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese
title_sort mind your assays: misleading cytotoxicity with the wst-1 assay in the presence of manganese
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161962/
https://www.ncbi.nlm.nih.gov/pubmed/32298350
http://dx.doi.org/10.1371/journal.pone.0231634
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