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Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment
Lemna species have been used in the food, feed, and pharmaceutical industries, as they are inexpensive sources of proteins, starches, and fatty acids. In this study, we treated L. paucicostata with different concentrations (0.05, 0.1, 0.2, 0.5, or 1 mM) of ethephon. The total dry weight decreased in...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162458/ https://www.ncbi.nlm.nih.gov/pubmed/32298342 http://dx.doi.org/10.1371/journal.pone.0231652 |
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author | Kim, EunBi Kim, Myeongsun Choi, Hyung-Kyoon |
author_facet | Kim, EunBi Kim, Myeongsun Choi, Hyung-Kyoon |
author_sort | Kim, EunBi |
collection | PubMed |
description | Lemna species have been used in the food, feed, and pharmaceutical industries, as they are inexpensive sources of proteins, starches, and fatty acids. In this study, we treated L. paucicostata with different concentrations (0.05, 0.1, 0.2, 0.5, or 1 mM) of ethephon. The total dry weight decreased in all ethephon-treated groups compared to the control group. We also investigated the alteration of metabolic profiles induced by ethephon treatment by using gas chromatography-mass spectrometry. This analysis identified 48 metabolites, and the relative levels of most of alcohols, amino acids, fatty acids, and phenols increased by the ethephon treatment, whereas levels of organic acids and sugars decreased. Among these, the highest production of γ-aminobutyric acid (GABA, 5.041 ± 1.373 mg/L) and ferulic acid (0.640 ± 0.071 mg/L) was observed in the 0.5 mM and the 0.2 mM ethephon treatment groups, respectively. These results could be useful for large-scale culture of L. paucicostata with enhanced GABA and ferulic acid content for utilization in the food, feed, cosmetic, and pharmaceutical industries. |
format | Online Article Text |
id | pubmed-7162458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-71624582020-04-21 Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment Kim, EunBi Kim, Myeongsun Choi, Hyung-Kyoon PLoS One Research Article Lemna species have been used in the food, feed, and pharmaceutical industries, as they are inexpensive sources of proteins, starches, and fatty acids. In this study, we treated L. paucicostata with different concentrations (0.05, 0.1, 0.2, 0.5, or 1 mM) of ethephon. The total dry weight decreased in all ethephon-treated groups compared to the control group. We also investigated the alteration of metabolic profiles induced by ethephon treatment by using gas chromatography-mass spectrometry. This analysis identified 48 metabolites, and the relative levels of most of alcohols, amino acids, fatty acids, and phenols increased by the ethephon treatment, whereas levels of organic acids and sugars decreased. Among these, the highest production of γ-aminobutyric acid (GABA, 5.041 ± 1.373 mg/L) and ferulic acid (0.640 ± 0.071 mg/L) was observed in the 0.5 mM and the 0.2 mM ethephon treatment groups, respectively. These results could be useful for large-scale culture of L. paucicostata with enhanced GABA and ferulic acid content for utilization in the food, feed, cosmetic, and pharmaceutical industries. Public Library of Science 2020-04-16 /pmc/articles/PMC7162458/ /pubmed/32298342 http://dx.doi.org/10.1371/journal.pone.0231652 Text en © 2020 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kim, EunBi Kim, Myeongsun Choi, Hyung-Kyoon Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment |
title | Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment |
title_full | Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment |
title_fullStr | Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment |
title_full_unstemmed | Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment |
title_short | Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment |
title_sort | alteration of metabolic profiles in lemna paucicostata culture and enhanced production of gaba and ferulic acid by ethephon treatment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162458/ https://www.ncbi.nlm.nih.gov/pubmed/32298342 http://dx.doi.org/10.1371/journal.pone.0231652 |
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