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The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway

Post-translationally modified peptides are involved in many aspects of plant growth and development. The maturation of these peptides from their larger precursors is still poorly understood. We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of pro...

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Autores principales: Stührwohldt, Nils, Scholl, Stefan, Lang, Lisa, Katzenberger, Julia, Schumacher, Karin, Schaller, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162652/
https://www.ncbi.nlm.nih.gov/pubmed/32297855
http://dx.doi.org/10.7554/eLife.55580
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author Stührwohldt, Nils
Scholl, Stefan
Lang, Lisa
Katzenberger, Julia
Schumacher, Karin
Schaller, Andreas
author_facet Stührwohldt, Nils
Scholl, Stefan
Lang, Lisa
Katzenberger, Julia
Schumacher, Karin
Schaller, Andreas
author_sort Stührwohldt, Nils
collection PubMed
description Post-translationally modified peptides are involved in many aspects of plant growth and development. The maturation of these peptides from their larger precursors is still poorly understood. We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of processing events in consecutive compartments of the secretory pathway. Following cleavage of the signal peptide upon entry into the endoplasmic reticulum (ER), the peptide precursors are processed in the cis-Golgi by the subtilase SBT6.1. SBT6.1-mediated cleavage within the variable domain allows for continued passage of the partially processed precursors through the secretory pathway, and for subsequent post-translational modifications including tyrosine sulfation and proline hydroxylation within, and proteolytic maturation after exit from the Golgi. Activation by subtilases including SBT3.8 in post-Golgi compartments depends on the N-terminal aspartate of the mature peptides. Our work highlights the complexity of post-translational precursor maturation allowing for stringent control of peptide biogenesis.
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spelling pubmed-71626522020-04-20 The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway Stührwohldt, Nils Scholl, Stefan Lang, Lisa Katzenberger, Julia Schumacher, Karin Schaller, Andreas eLife Plant Biology Post-translationally modified peptides are involved in many aspects of plant growth and development. The maturation of these peptides from their larger precursors is still poorly understood. We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of processing events in consecutive compartments of the secretory pathway. Following cleavage of the signal peptide upon entry into the endoplasmic reticulum (ER), the peptide precursors are processed in the cis-Golgi by the subtilase SBT6.1. SBT6.1-mediated cleavage within the variable domain allows for continued passage of the partially processed precursors through the secretory pathway, and for subsequent post-translational modifications including tyrosine sulfation and proline hydroxylation within, and proteolytic maturation after exit from the Golgi. Activation by subtilases including SBT3.8 in post-Golgi compartments depends on the N-terminal aspartate of the mature peptides. Our work highlights the complexity of post-translational precursor maturation allowing for stringent control of peptide biogenesis. eLife Sciences Publications, Ltd 2020-04-16 /pmc/articles/PMC7162652/ /pubmed/32297855 http://dx.doi.org/10.7554/eLife.55580 Text en © 2020, Stührwohldt et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Plant Biology
Stührwohldt, Nils
Scholl, Stefan
Lang, Lisa
Katzenberger, Julia
Schumacher, Karin
Schaller, Andreas
The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway
title The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway
title_full The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway
title_fullStr The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway
title_full_unstemmed The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway
title_short The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway
title_sort biogenesis of clel peptides involves several processing events in consecutive compartments of the secretory pathway
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162652/
https://www.ncbi.nlm.nih.gov/pubmed/32297855
http://dx.doi.org/10.7554/eLife.55580
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