Cargando…
The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway
Post-translationally modified peptides are involved in many aspects of plant growth and development. The maturation of these peptides from their larger precursors is still poorly understood. We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of pro...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162652/ https://www.ncbi.nlm.nih.gov/pubmed/32297855 http://dx.doi.org/10.7554/eLife.55580 |
_version_ | 1783523073188691968 |
---|---|
author | Stührwohldt, Nils Scholl, Stefan Lang, Lisa Katzenberger, Julia Schumacher, Karin Schaller, Andreas |
author_facet | Stührwohldt, Nils Scholl, Stefan Lang, Lisa Katzenberger, Julia Schumacher, Karin Schaller, Andreas |
author_sort | Stührwohldt, Nils |
collection | PubMed |
description | Post-translationally modified peptides are involved in many aspects of plant growth and development. The maturation of these peptides from their larger precursors is still poorly understood. We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of processing events in consecutive compartments of the secretory pathway. Following cleavage of the signal peptide upon entry into the endoplasmic reticulum (ER), the peptide precursors are processed in the cis-Golgi by the subtilase SBT6.1. SBT6.1-mediated cleavage within the variable domain allows for continued passage of the partially processed precursors through the secretory pathway, and for subsequent post-translational modifications including tyrosine sulfation and proline hydroxylation within, and proteolytic maturation after exit from the Golgi. Activation by subtilases including SBT3.8 in post-Golgi compartments depends on the N-terminal aspartate of the mature peptides. Our work highlights the complexity of post-translational precursor maturation allowing for stringent control of peptide biogenesis. |
format | Online Article Text |
id | pubmed-7162652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-71626522020-04-20 The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway Stührwohldt, Nils Scholl, Stefan Lang, Lisa Katzenberger, Julia Schumacher, Karin Schaller, Andreas eLife Plant Biology Post-translationally modified peptides are involved in many aspects of plant growth and development. The maturation of these peptides from their larger precursors is still poorly understood. We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of processing events in consecutive compartments of the secretory pathway. Following cleavage of the signal peptide upon entry into the endoplasmic reticulum (ER), the peptide precursors are processed in the cis-Golgi by the subtilase SBT6.1. SBT6.1-mediated cleavage within the variable domain allows for continued passage of the partially processed precursors through the secretory pathway, and for subsequent post-translational modifications including tyrosine sulfation and proline hydroxylation within, and proteolytic maturation after exit from the Golgi. Activation by subtilases including SBT3.8 in post-Golgi compartments depends on the N-terminal aspartate of the mature peptides. Our work highlights the complexity of post-translational precursor maturation allowing for stringent control of peptide biogenesis. eLife Sciences Publications, Ltd 2020-04-16 /pmc/articles/PMC7162652/ /pubmed/32297855 http://dx.doi.org/10.7554/eLife.55580 Text en © 2020, Stührwohldt et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Plant Biology Stührwohldt, Nils Scholl, Stefan Lang, Lisa Katzenberger, Julia Schumacher, Karin Schaller, Andreas The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway |
title | The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway |
title_full | The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway |
title_fullStr | The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway |
title_full_unstemmed | The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway |
title_short | The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway |
title_sort | biogenesis of clel peptides involves several processing events in consecutive compartments of the secretory pathway |
topic | Plant Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162652/ https://www.ncbi.nlm.nih.gov/pubmed/32297855 http://dx.doi.org/10.7554/eLife.55580 |
work_keys_str_mv | AT stuhrwohldtnils thebiogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT schollstefan thebiogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT langlisa thebiogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT katzenbergerjulia thebiogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT schumacherkarin thebiogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT schallerandreas thebiogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT stuhrwohldtnils biogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT schollstefan biogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT langlisa biogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT katzenbergerjulia biogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT schumacherkarin biogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway AT schallerandreas biogenesisofclelpeptidesinvolvesseveralprocessingeventsinconsecutivecompartmentsofthesecretorypathway |