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RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia
In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formatio...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162659/ https://www.ncbi.nlm.nih.gov/pubmed/32298230 http://dx.doi.org/10.7554/eLife.48480 |
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author | Fuller, Gregory G Han, Ting Freeberg, Mallory A Moresco, James J Ghanbari Niaki, Amirhossein Roach, Nathan P Yates, John R Myong, Sua Kim, John K |
author_facet | Fuller, Gregory G Han, Ting Freeberg, Mallory A Moresco, James J Ghanbari Niaki, Amirhossein Roach, Nathan P Yates, John R Myong, Sua Kim, John K |
author_sort | Fuller, Gregory G |
collection | PubMed |
description | In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We demonstrate that glycolysis enzymes are non-canonical RNA binding proteins, sharing many common mRNA substrates that are also integral constituents of G bodies. Targeting nonspecific endoribonucleases to G bodies reveals that RNA nucleates G body formation and maintains its structural integrity. Consistent with a phase separation mechanism of biogenesis, recruitment of glycolysis enzymes to G bodies relies on multivalent homotypic and heterotypic interactions. Furthermore, G bodies fuse in vivo and are largely insensitive to 1,6-hexanediol, consistent with a hydrogel-like composition. Taken together, our results elucidate the biophysical nature of G bodies and demonstrate that RNA nucleates phase separation of the glycolysis machinery in response to hypoxic stress. |
format | Online Article Text |
id | pubmed-7162659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-71626592020-04-20 RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia Fuller, Gregory G Han, Ting Freeberg, Mallory A Moresco, James J Ghanbari Niaki, Amirhossein Roach, Nathan P Yates, John R Myong, Sua Kim, John K eLife Cell Biology In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We demonstrate that glycolysis enzymes are non-canonical RNA binding proteins, sharing many common mRNA substrates that are also integral constituents of G bodies. Targeting nonspecific endoribonucleases to G bodies reveals that RNA nucleates G body formation and maintains its structural integrity. Consistent with a phase separation mechanism of biogenesis, recruitment of glycolysis enzymes to G bodies relies on multivalent homotypic and heterotypic interactions. Furthermore, G bodies fuse in vivo and are largely insensitive to 1,6-hexanediol, consistent with a hydrogel-like composition. Taken together, our results elucidate the biophysical nature of G bodies and demonstrate that RNA nucleates phase separation of the glycolysis machinery in response to hypoxic stress. eLife Sciences Publications, Ltd 2020-04-16 /pmc/articles/PMC7162659/ /pubmed/32298230 http://dx.doi.org/10.7554/eLife.48480 Text en © 2020, Fuller et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Fuller, Gregory G Han, Ting Freeberg, Mallory A Moresco, James J Ghanbari Niaki, Amirhossein Roach, Nathan P Yates, John R Myong, Sua Kim, John K RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia |
title | RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia |
title_full | RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia |
title_fullStr | RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia |
title_full_unstemmed | RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia |
title_short | RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia |
title_sort | rna promotes phase separation of glycolysis enzymes into yeast g bodies in hypoxia |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162659/ https://www.ncbi.nlm.nih.gov/pubmed/32298230 http://dx.doi.org/10.7554/eLife.48480 |
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