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Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F)
OBJECTIVES: Alzheimer's disease (AD) is the most common neurodegenerative disease which is characterized by the formation of amyloid beta (Aβ) plaques and neurofibrillary tangles. These abnormal proteins induce disturbance in mitochondrial dynamics and defect in autophagy system. Since presenil...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162796/ https://www.ncbi.nlm.nih.gov/pubmed/32216003 http://dx.doi.org/10.1111/cpr.12798 |
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author | Li, Ling Kim, Hee Jin Roh, Jee Hoon Kim, Minchul Koh, Wonyoung Kim, Younghoon Heo, Hyohoon Chung, Jaehoon Nakanishi, Mahito Yoon, Taeyoung Hong, Chang Pyo Seo, Sang Won Na, Duk L. Song, Jihwan |
author_facet | Li, Ling Kim, Hee Jin Roh, Jee Hoon Kim, Minchul Koh, Wonyoung Kim, Younghoon Heo, Hyohoon Chung, Jaehoon Nakanishi, Mahito Yoon, Taeyoung Hong, Chang Pyo Seo, Sang Won Na, Duk L. Song, Jihwan |
author_sort | Li, Ling |
collection | PubMed |
description | OBJECTIVES: Alzheimer's disease (AD) is the most common neurodegenerative disease which is characterized by the formation of amyloid beta (Aβ) plaques and neurofibrillary tangles. These abnormal proteins induce disturbance in mitochondrial dynamics and defect in autophagy system. Since presenilin‐1 (PS1) is a core component in γ‐secretase complex, the mutations of PS1 gene cause the interference of γ‐secretase activity and lead to the increased Aβ(42) secretion. We aimed to characterize the patient‐specific induced pluripotent stem cell (iPSC) line carrying PS1‐S170F mutation. Furthermore, we tested whether disease‐modifying drug can reduce AD pathology in the AD iPSC‐derived neurons. MATERIALS AND METHODS: Mononuclear cells (MNCs) were isolated freshly from the peripheral blood of an autosomal dominant AD (ADAD) patient carrying presenilin‐1 (PS1) mutation (Ser170Phe; PS1‐S170F) and a cognitively normal control. We generated induced pluripotent stem cell (iPSC) lines, which were differentiated into functional cortical neurons. Then, we measured the markers indicative of AD pathogenesis using immunocytochemistry and Western blot. We also investigated the mitochondrial dynamics in the AD iPSC‐derived neurons using Mito‐tracker. RESULTS: We observed that both extracellular and intracellular Aβ levels were dramatically increased in the PS1‐S170F iPSC‐derived neurons, compared with the control iPSC‐derived neurons. Furthermore, PS1‐S170F iPSC‐derived neurons showed high expression levels of p‐Tau, which were detected both in the soma and neurites. The mitochondrial velocity in the PS1‐S170F iPSC‐derived neurons was much reduced, compared with that of the control. We also found a significant decrease of fusion‐related protein Mfn1 (membrane proteins mitofusin 1) and an increase of fission‐related protein DRP1 (dynamin‐related protein 1) in the PS1‐S170F iPSC‐derived neurons. We further observed the defects of autophagy‐related clearance in the PS1‐S170F iPSC‐derived neurons. Finally, we demonstrated the levels of Aβ and p‐Tau can be dramatically reduced by the treatment of LY‐2886721, a BACE1 inhibitor. CONCLUSIONS: Taken together, we have established and characterized the pathological features of an AD patient carrying PS1‐S170F mutation using iPSC technology, which will be the first case on this mutation and this iPSC line will serve as a useful resource for studying AD pathogenesis and drug screening in the future. |
format | Online Article Text |
id | pubmed-7162796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71627962020-04-20 Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F) Li, Ling Kim, Hee Jin Roh, Jee Hoon Kim, Minchul Koh, Wonyoung Kim, Younghoon Heo, Hyohoon Chung, Jaehoon Nakanishi, Mahito Yoon, Taeyoung Hong, Chang Pyo Seo, Sang Won Na, Duk L. Song, Jihwan Cell Prolif Original Articles OBJECTIVES: Alzheimer's disease (AD) is the most common neurodegenerative disease which is characterized by the formation of amyloid beta (Aβ) plaques and neurofibrillary tangles. These abnormal proteins induce disturbance in mitochondrial dynamics and defect in autophagy system. Since presenilin‐1 (PS1) is a core component in γ‐secretase complex, the mutations of PS1 gene cause the interference of γ‐secretase activity and lead to the increased Aβ(42) secretion. We aimed to characterize the patient‐specific induced pluripotent stem cell (iPSC) line carrying PS1‐S170F mutation. Furthermore, we tested whether disease‐modifying drug can reduce AD pathology in the AD iPSC‐derived neurons. MATERIALS AND METHODS: Mononuclear cells (MNCs) were isolated freshly from the peripheral blood of an autosomal dominant AD (ADAD) patient carrying presenilin‐1 (PS1) mutation (Ser170Phe; PS1‐S170F) and a cognitively normal control. We generated induced pluripotent stem cell (iPSC) lines, which were differentiated into functional cortical neurons. Then, we measured the markers indicative of AD pathogenesis using immunocytochemistry and Western blot. We also investigated the mitochondrial dynamics in the AD iPSC‐derived neurons using Mito‐tracker. RESULTS: We observed that both extracellular and intracellular Aβ levels were dramatically increased in the PS1‐S170F iPSC‐derived neurons, compared with the control iPSC‐derived neurons. Furthermore, PS1‐S170F iPSC‐derived neurons showed high expression levels of p‐Tau, which were detected both in the soma and neurites. The mitochondrial velocity in the PS1‐S170F iPSC‐derived neurons was much reduced, compared with that of the control. We also found a significant decrease of fusion‐related protein Mfn1 (membrane proteins mitofusin 1) and an increase of fission‐related protein DRP1 (dynamin‐related protein 1) in the PS1‐S170F iPSC‐derived neurons. We further observed the defects of autophagy‐related clearance in the PS1‐S170F iPSC‐derived neurons. Finally, we demonstrated the levels of Aβ and p‐Tau can be dramatically reduced by the treatment of LY‐2886721, a BACE1 inhibitor. CONCLUSIONS: Taken together, we have established and characterized the pathological features of an AD patient carrying PS1‐S170F mutation using iPSC technology, which will be the first case on this mutation and this iPSC line will serve as a useful resource for studying AD pathogenesis and drug screening in the future. John Wiley and Sons Inc. 2020-03-25 /pmc/articles/PMC7162796/ /pubmed/32216003 http://dx.doi.org/10.1111/cpr.12798 Text en © 2020 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Ling Kim, Hee Jin Roh, Jee Hoon Kim, Minchul Koh, Wonyoung Kim, Younghoon Heo, Hyohoon Chung, Jaehoon Nakanishi, Mahito Yoon, Taeyoung Hong, Chang Pyo Seo, Sang Won Na, Duk L. Song, Jihwan Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F) |
title | Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F) |
title_full | Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F) |
title_fullStr | Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F) |
title_full_unstemmed | Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F) |
title_short | Pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset Alzheimer's disease patient carrying a presenilin‐1 mutation (S170F) |
title_sort | pathological manifestation of the induced pluripotent stem cell‐derived cortical neurons from an early‐onset alzheimer's disease patient carrying a presenilin‐1 mutation (s170f) |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162796/ https://www.ncbi.nlm.nih.gov/pubmed/32216003 http://dx.doi.org/10.1111/cpr.12798 |
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