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Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer

Helicobacter pylori (HP) colonizes the human stomach and induces acute gastritis, peptic ulcer disease, atrophic gastritis, and gastric adenocarcinoma. Increased virulence in HP isolates derives from harboring the cag (cytotoxin-associated genes) pathogenicity island (cagPAI). We analyzed the microv...

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Autores principales: Rizzato, Cosmeri, Torres, Javier, Obazee, Ofure, Camorlinga-Ponce, Margarita, Trujillo, Esperanza, Stein, Angelika, Mendez-Tenorio, Alfonso, Bravo, Maria Mercedes, Canzian, Federico, Kato, Ikuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162905/
https://www.ncbi.nlm.nih.gov/pubmed/32300197
http://dx.doi.org/10.1038/s41598-020-63463-0
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author Rizzato, Cosmeri
Torres, Javier
Obazee, Ofure
Camorlinga-Ponce, Margarita
Trujillo, Esperanza
Stein, Angelika
Mendez-Tenorio, Alfonso
Bravo, Maria Mercedes
Canzian, Federico
Kato, Ikuko
author_facet Rizzato, Cosmeri
Torres, Javier
Obazee, Ofure
Camorlinga-Ponce, Margarita
Trujillo, Esperanza
Stein, Angelika
Mendez-Tenorio, Alfonso
Bravo, Maria Mercedes
Canzian, Federico
Kato, Ikuko
author_sort Rizzato, Cosmeri
collection PubMed
description Helicobacter pylori (HP) colonizes the human stomach and induces acute gastritis, peptic ulcer disease, atrophic gastritis, and gastric adenocarcinoma. Increased virulence in HP isolates derives from harboring the cag (cytotoxin-associated genes) pathogenicity island (cagPAI). We analyzed the microvariants in cagPAI genes with the hypothesis that they may play an important role in determining HP virulence. We tested DNAs from cagA positive patients HP isolates; a total of 74 patients with chronic gastritis (CG, N = 37), intestinal metaplasia (IM, N = 21) or gastric cancer (GC, N = 16) from Mexico and Colombia. We selected 520 non-synonymous variants with at least 7.5% frequency in the original sequence outputs or with a minimum of 5 isolates with minor allele. After adjustment for multiple comparisons, no variants were statistically significantly associated with IM or GC. However, 19 non-synonymous showed conventional P-values < 0.05 comparing the frequency of the alleles between the isolates from subjects with gastritis and isolates from subjects with IM or GC; 12 of these showed a significant correlation with the severity of the disease. The present study revealed that several cagPAI genes from Latin American Western HP strains contains a number of non-synonymous variants in relatively high frequencies which could influence on the clinical outcome. However, none of the associations remained statistically significant after adjustment for multiple comparison.
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spelling pubmed-71629052020-04-22 Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer Rizzato, Cosmeri Torres, Javier Obazee, Ofure Camorlinga-Ponce, Margarita Trujillo, Esperanza Stein, Angelika Mendez-Tenorio, Alfonso Bravo, Maria Mercedes Canzian, Federico Kato, Ikuko Sci Rep Article Helicobacter pylori (HP) colonizes the human stomach and induces acute gastritis, peptic ulcer disease, atrophic gastritis, and gastric adenocarcinoma. Increased virulence in HP isolates derives from harboring the cag (cytotoxin-associated genes) pathogenicity island (cagPAI). We analyzed the microvariants in cagPAI genes with the hypothesis that they may play an important role in determining HP virulence. We tested DNAs from cagA positive patients HP isolates; a total of 74 patients with chronic gastritis (CG, N = 37), intestinal metaplasia (IM, N = 21) or gastric cancer (GC, N = 16) from Mexico and Colombia. We selected 520 non-synonymous variants with at least 7.5% frequency in the original sequence outputs or with a minimum of 5 isolates with minor allele. After adjustment for multiple comparisons, no variants were statistically significantly associated with IM or GC. However, 19 non-synonymous showed conventional P-values < 0.05 comparing the frequency of the alleles between the isolates from subjects with gastritis and isolates from subjects with IM or GC; 12 of these showed a significant correlation with the severity of the disease. The present study revealed that several cagPAI genes from Latin American Western HP strains contains a number of non-synonymous variants in relatively high frequencies which could influence on the clinical outcome. However, none of the associations remained statistically significant after adjustment for multiple comparison. Nature Publishing Group UK 2020-04-16 /pmc/articles/PMC7162905/ /pubmed/32300197 http://dx.doi.org/10.1038/s41598-020-63463-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rizzato, Cosmeri
Torres, Javier
Obazee, Ofure
Camorlinga-Ponce, Margarita
Trujillo, Esperanza
Stein, Angelika
Mendez-Tenorio, Alfonso
Bravo, Maria Mercedes
Canzian, Federico
Kato, Ikuko
Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer
title Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer
title_full Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer
title_fullStr Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer
title_full_unstemmed Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer
title_short Variations in cag pathogenicity island genes of Helicobacter pylori from Latin American groups may influence neoplastic progression to gastric cancer
title_sort variations in cag pathogenicity island genes of helicobacter pylori from latin american groups may influence neoplastic progression to gastric cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162905/
https://www.ncbi.nlm.nih.gov/pubmed/32300197
http://dx.doi.org/10.1038/s41598-020-63463-0
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