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IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis

Dupilumab is a fully human antibody to interleukin-4 receptor α that improves the signs and symptoms of moderate to severe atopic dermatitis (AD). To determine the effects of dupilumab on Staphylococcus aureus colonization and microbial diversity on the skin, bacterial DNA was analyzed from swabs co...

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Autores principales: Callewaert, Chris, Nakatsuji, Teruaki, Knight, Rob, Kosciolek, Tomasz, Vrbanac, Alison, Kotol, Paul, Ardeleanu, Marius, Hultsch, Thomas, Guttman-Yassky, Emma, Bissonnette, Robert, Silverberg, Jonathan I., Krueger, James, Menter, Alan, Graham, Neil M.H., Pirozzi, Gianluca, Hamilton, Jennifer D., Gallo, Richard L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7163930/
https://www.ncbi.nlm.nih.gov/pubmed/31252032
http://dx.doi.org/10.1016/j.jid.2019.05.024
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author Callewaert, Chris
Nakatsuji, Teruaki
Knight, Rob
Kosciolek, Tomasz
Vrbanac, Alison
Kotol, Paul
Ardeleanu, Marius
Hultsch, Thomas
Guttman-Yassky, Emma
Bissonnette, Robert
Silverberg, Jonathan I.
Krueger, James
Menter, Alan
Graham, Neil M.H.
Pirozzi, Gianluca
Hamilton, Jennifer D.
Gallo, Richard L.
author_facet Callewaert, Chris
Nakatsuji, Teruaki
Knight, Rob
Kosciolek, Tomasz
Vrbanac, Alison
Kotol, Paul
Ardeleanu, Marius
Hultsch, Thomas
Guttman-Yassky, Emma
Bissonnette, Robert
Silverberg, Jonathan I.
Krueger, James
Menter, Alan
Graham, Neil M.H.
Pirozzi, Gianluca
Hamilton, Jennifer D.
Gallo, Richard L.
author_sort Callewaert, Chris
collection PubMed
description Dupilumab is a fully human antibody to interleukin-4 receptor α that improves the signs and symptoms of moderate to severe atopic dermatitis (AD). To determine the effects of dupilumab on Staphylococcus aureus colonization and microbial diversity on the skin, bacterial DNA was analyzed from swabs collected from lesional and nonlesional skin in a double-blind, placebo-controlled study of 54 patients with moderate to severe AD randomized (1:1) and treated with either dupilumab (200 mg weekly) or placebo for 16 weeks. Microbial diversity and relative abundance of Staphylococcus were assessed by DNA sequencing of 16S ribosomal RNA, and absolute S. aureus abundance was measured by quantitative PCR. Before treatment, lesional skin had lower microbial diversity and higher overall abundance of S. aureus than nonlesional skin. During dupilumab treatment, microbial diversity increased and the abundance of S. aureus decreased. Pronounced changes were seen in nonlesional and lesional skin. Decreased S. aureus abundance during dupilumab treatment correlated with clinical improvement of AD and biomarkers of type 2 immunity. We conclude that clinical improvement of AD that is mediated by interleukin-4 receptor α inhibition and the subsequent suppression of type 2 inflammation is correlated with increased microbial diversity and reduced abundance of S. aureus.
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spelling pubmed-71639302020-04-17 IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis Callewaert, Chris Nakatsuji, Teruaki Knight, Rob Kosciolek, Tomasz Vrbanac, Alison Kotol, Paul Ardeleanu, Marius Hultsch, Thomas Guttman-Yassky, Emma Bissonnette, Robert Silverberg, Jonathan I. Krueger, James Menter, Alan Graham, Neil M.H. Pirozzi, Gianluca Hamilton, Jennifer D. Gallo, Richard L. J Invest Dermatol Article Dupilumab is a fully human antibody to interleukin-4 receptor α that improves the signs and symptoms of moderate to severe atopic dermatitis (AD). To determine the effects of dupilumab on Staphylococcus aureus colonization and microbial diversity on the skin, bacterial DNA was analyzed from swabs collected from lesional and nonlesional skin in a double-blind, placebo-controlled study of 54 patients with moderate to severe AD randomized (1:1) and treated with either dupilumab (200 mg weekly) or placebo for 16 weeks. Microbial diversity and relative abundance of Staphylococcus were assessed by DNA sequencing of 16S ribosomal RNA, and absolute S. aureus abundance was measured by quantitative PCR. Before treatment, lesional skin had lower microbial diversity and higher overall abundance of S. aureus than nonlesional skin. During dupilumab treatment, microbial diversity increased and the abundance of S. aureus decreased. Pronounced changes were seen in nonlesional and lesional skin. Decreased S. aureus abundance during dupilumab treatment correlated with clinical improvement of AD and biomarkers of type 2 immunity. We conclude that clinical improvement of AD that is mediated by interleukin-4 receptor α inhibition and the subsequent suppression of type 2 inflammation is correlated with increased microbial diversity and reduced abundance of S. aureus. 2019-06-25 2020-01 /pmc/articles/PMC7163930/ /pubmed/31252032 http://dx.doi.org/10.1016/j.jid.2019.05.024 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. To view a copy of this license, visit
spellingShingle Article
Callewaert, Chris
Nakatsuji, Teruaki
Knight, Rob
Kosciolek, Tomasz
Vrbanac, Alison
Kotol, Paul
Ardeleanu, Marius
Hultsch, Thomas
Guttman-Yassky, Emma
Bissonnette, Robert
Silverberg, Jonathan I.
Krueger, James
Menter, Alan
Graham, Neil M.H.
Pirozzi, Gianluca
Hamilton, Jennifer D.
Gallo, Richard L.
IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis
title IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis
title_full IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis
title_fullStr IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis
title_full_unstemmed IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis
title_short IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis
title_sort il-4rα blockade by dupilumab decreases staphylococcus aureus colonization and increases microbial diversity in atopic dermatitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7163930/
https://www.ncbi.nlm.nih.gov/pubmed/31252032
http://dx.doi.org/10.1016/j.jid.2019.05.024
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