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Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies

Scabies is a highly contagious parasitic disease associated with long-term residence in nursing homes, and it is a public health burden worldwide. However, atypical skin manifestations are frequent and the widely used diagnostic test based on microscopic examinations has limited sensitivity. We eval...

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Autores principales: Bae, Moonsuk, Kim, Ji Yeun, Jung, Jiwon, Cha, Hye Hee, Jeon, Na-Young, Lee, Hyun-Jung, Kim, Min Jae, Chang, Sung Eun, Kim, Sung-Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164670/
https://www.ncbi.nlm.nih.gov/pubmed/32255795
http://dx.doi.org/10.1371/journal.pntd.0008229
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author Bae, Moonsuk
Kim, Ji Yeun
Jung, Jiwon
Cha, Hye Hee
Jeon, Na-Young
Lee, Hyun-Jung
Kim, Min Jae
Chang, Sung Eun
Kim, Sung-Han
author_facet Bae, Moonsuk
Kim, Ji Yeun
Jung, Jiwon
Cha, Hye Hee
Jeon, Na-Young
Lee, Hyun-Jung
Kim, Min Jae
Chang, Sung Eun
Kim, Sung-Han
author_sort Bae, Moonsuk
collection PubMed
description Scabies is a highly contagious parasitic disease associated with long-term residence in nursing homes, and it is a public health burden worldwide. However, atypical skin manifestations are frequent and the widely used diagnostic test based on microscopic examinations has limited sensitivity. We evaluated the diagnostic value of polymerase chain reaction (PCR) from skin scraping in patients with suspected scabies. Adult patients with suspected scabies, unrelated diseases or healthy volunteers were enrolled at a tertiary hospital, in Seoul, South Korea, from December 2017 through October 2018. We classified participants based on the consensus criteria established by the International Alliance for the Control of Scabies in 2018; confirmed (microscopic mite detection), clinical (scabies burrow or typical lesions with two history features including itch and close contact with scabies patients), suspected scabies (typical lesion with one history feature or atypical lesion with two history features), or no scabies. PCR was performed on the skin scrapings to target the cytochrome c oxidase subunit 1 (cox1) gene of Sarcoptes scabiei. A total of 47 participants, 33 with suspected scabies, 10 with unrelated diseases, and 4 healthy volunteers were enrolled. Of the 33 patients, 22 were classified as confirmed scabies, 2 as clinical scabies, 6 as suspected scabies, and 3 as no scabies. The sensitivities of the microscopic examination were 100%, 92%, and 73% in confirmed scabies; confirmed and clinical scabies; and confirmed, clinical, and suspected scabies, respectively (p = 0.006). The sensitivities of PCR were 86%, 83%, and 80% in confirmed scabies; confirmed and clinical scabies; and confirmed, clinical, and suspected scabies, respectively (p = 0.59). The specificity of the scabies PCR in the no scabies control was 100% (95% CI = 80–100).PCR testing for scabies may be helpful in the improvement of sensitivity for the diagnosis of scabies by clinical criteria.
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spelling pubmed-71646702020-04-22 Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies Bae, Moonsuk Kim, Ji Yeun Jung, Jiwon Cha, Hye Hee Jeon, Na-Young Lee, Hyun-Jung Kim, Min Jae Chang, Sung Eun Kim, Sung-Han PLoS Negl Trop Dis Research Article Scabies is a highly contagious parasitic disease associated with long-term residence in nursing homes, and it is a public health burden worldwide. However, atypical skin manifestations are frequent and the widely used diagnostic test based on microscopic examinations has limited sensitivity. We evaluated the diagnostic value of polymerase chain reaction (PCR) from skin scraping in patients with suspected scabies. Adult patients with suspected scabies, unrelated diseases or healthy volunteers were enrolled at a tertiary hospital, in Seoul, South Korea, from December 2017 through October 2018. We classified participants based on the consensus criteria established by the International Alliance for the Control of Scabies in 2018; confirmed (microscopic mite detection), clinical (scabies burrow or typical lesions with two history features including itch and close contact with scabies patients), suspected scabies (typical lesion with one history feature or atypical lesion with two history features), or no scabies. PCR was performed on the skin scrapings to target the cytochrome c oxidase subunit 1 (cox1) gene of Sarcoptes scabiei. A total of 47 participants, 33 with suspected scabies, 10 with unrelated diseases, and 4 healthy volunteers were enrolled. Of the 33 patients, 22 were classified as confirmed scabies, 2 as clinical scabies, 6 as suspected scabies, and 3 as no scabies. The sensitivities of the microscopic examination were 100%, 92%, and 73% in confirmed scabies; confirmed and clinical scabies; and confirmed, clinical, and suspected scabies, respectively (p = 0.006). The sensitivities of PCR were 86%, 83%, and 80% in confirmed scabies; confirmed and clinical scabies; and confirmed, clinical, and suspected scabies, respectively (p = 0.59). The specificity of the scabies PCR in the no scabies control was 100% (95% CI = 80–100).PCR testing for scabies may be helpful in the improvement of sensitivity for the diagnosis of scabies by clinical criteria. Public Library of Science 2020-04-07 /pmc/articles/PMC7164670/ /pubmed/32255795 http://dx.doi.org/10.1371/journal.pntd.0008229 Text en © 2020 Bae et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bae, Moonsuk
Kim, Ji Yeun
Jung, Jiwon
Cha, Hye Hee
Jeon, Na-Young
Lee, Hyun-Jung
Kim, Min Jae
Chang, Sung Eun
Kim, Sung-Han
Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies
title Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies
title_full Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies
title_fullStr Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies
title_full_unstemmed Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies
title_short Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies
title_sort diagnostic value of the molecular detection of sarcoptes scabiei from a skin scraping in patients with suspected scabies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164670/
https://www.ncbi.nlm.nih.gov/pubmed/32255795
http://dx.doi.org/10.1371/journal.pntd.0008229
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