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Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia

The high prevalence of Toxoplasma gondii in the human population in Colombia has been linked to the existence of a high density of urban stray cats, exposing the whole population to a high density of oocysts. The goal of this study was to determine the DNA prevalence of T. gondii by conventional PCR...

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Autores principales: Zamora-Vélez, Alejandro, Triviño, Jessica, Cuadrado-Ríos, Sebastián, Lora-Suarez, Fabiana, Enrique Gómez-Marín, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164795/
https://www.ncbi.nlm.nih.gov/pubmed/32302287
http://dx.doi.org/10.1051/parasite/2020023
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author Zamora-Vélez, Alejandro
Triviño, Jessica
Cuadrado-Ríos, Sebastián
Lora-Suarez, Fabiana
Enrique Gómez-Marín, Jorge
author_facet Zamora-Vélez, Alejandro
Triviño, Jessica
Cuadrado-Ríos, Sebastián
Lora-Suarez, Fabiana
Enrique Gómez-Marín, Jorge
author_sort Zamora-Vélez, Alejandro
collection PubMed
description The high prevalence of Toxoplasma gondii in the human population in Colombia has been linked to the existence of a high density of urban stray cats, exposing the whole population to a high density of oocysts. The goal of this study was to determine the DNA prevalence of T. gondii by conventional PCR and to phylogenetically analyze ROP18 sequences from positive samples in domestic cat (Felis catus) fecal samples in the city of Armenia, Quindío. Fecal samples from 140 cats were collected from 10 districts around the city. Samples were concentrated using Ritchie’s method and analyzed through optical microscopy. Concentrates were used for DNA extraction followed by nested PCR amplification for T. gondii gene B1. PCR for ROP18 was performed on all B1 positive samples; the ROP18 sequences obtained were related to the Archetype I Brazilian and Chinese strains. No oocysts were detected by optical microscopy; however, 17.8% (25/140) B1 and 24% (6/25) ROP18 PCR-positive samples were detected. Phylogenetic analyses showed that isolates clustered into a single group. We assessed whether associations existed between T. gondii positive fecal samples and survey variables such as cat healthcare and socioeconomic characteristics of owners, but no statistically significant associations were found. The presence of T. gondii in cat feces is an important factor contributing to the high prevalence in the human population of this city.
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spelling pubmed-71647952020-04-21 Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia Zamora-Vélez, Alejandro Triviño, Jessica Cuadrado-Ríos, Sebastián Lora-Suarez, Fabiana Enrique Gómez-Marín, Jorge Parasite Research Article The high prevalence of Toxoplasma gondii in the human population in Colombia has been linked to the existence of a high density of urban stray cats, exposing the whole population to a high density of oocysts. The goal of this study was to determine the DNA prevalence of T. gondii by conventional PCR and to phylogenetically analyze ROP18 sequences from positive samples in domestic cat (Felis catus) fecal samples in the city of Armenia, Quindío. Fecal samples from 140 cats were collected from 10 districts around the city. Samples were concentrated using Ritchie’s method and analyzed through optical microscopy. Concentrates were used for DNA extraction followed by nested PCR amplification for T. gondii gene B1. PCR for ROP18 was performed on all B1 positive samples; the ROP18 sequences obtained were related to the Archetype I Brazilian and Chinese strains. No oocysts were detected by optical microscopy; however, 17.8% (25/140) B1 and 24% (6/25) ROP18 PCR-positive samples were detected. Phylogenetic analyses showed that isolates clustered into a single group. We assessed whether associations existed between T. gondii positive fecal samples and survey variables such as cat healthcare and socioeconomic characteristics of owners, but no statistically significant associations were found. The presence of T. gondii in cat feces is an important factor contributing to the high prevalence in the human population of this city. EDP Sciences 2020-04-17 /pmc/articles/PMC7164795/ /pubmed/32302287 http://dx.doi.org/10.1051/parasite/2020023 Text en © A. Zamora-Vélez et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zamora-Vélez, Alejandro
Triviño, Jessica
Cuadrado-Ríos, Sebastián
Lora-Suarez, Fabiana
Enrique Gómez-Marín, Jorge
Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia
title Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia
title_full Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia
title_fullStr Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia
title_full_unstemmed Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia
title_short Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia
title_sort detection and genotypes of toxoplasma gondii dna in feces of domestic cats in colombia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164795/
https://www.ncbi.nlm.nih.gov/pubmed/32302287
http://dx.doi.org/10.1051/parasite/2020023
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