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The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser
Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, and bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation of the protein through structural changes. We present serial femtosecond X-ray crystallographic data of...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164956/ https://www.ncbi.nlm.nih.gov/pubmed/32228856 http://dx.doi.org/10.7554/eLife.53514 |
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author | Claesson, Elin Wahlgren, Weixiao Yuan Takala, Heikki Pandey, Suraj Castillon, Leticia Kuznetsova, Valentyna Henry, Léocadie Panman, Matthijs Carrillo, Melissa Kübel, Joachim Nanekar, Rahul Isaksson, Linnéa Nimmrich, Amke Cellini, Andrea Morozov, Dmitry Maj, Michał Kurttila, Moona Bosman, Robert Nango, Eriko Tanaka, Rie Tanaka, Tomoyuki Fangjia, Luo Iwata, So Owada, Shigeki Moffat, Keith Groenhof, Gerrit Stojković, Emina A Ihalainen, Janne A Schmidt, Marius Westenhoff, Sebastian |
author_facet | Claesson, Elin Wahlgren, Weixiao Yuan Takala, Heikki Pandey, Suraj Castillon, Leticia Kuznetsova, Valentyna Henry, Léocadie Panman, Matthijs Carrillo, Melissa Kübel, Joachim Nanekar, Rahul Isaksson, Linnéa Nimmrich, Amke Cellini, Andrea Morozov, Dmitry Maj, Michał Kurttila, Moona Bosman, Robert Nango, Eriko Tanaka, Rie Tanaka, Tomoyuki Fangjia, Luo Iwata, So Owada, Shigeki Moffat, Keith Groenhof, Gerrit Stojković, Emina A Ihalainen, Janne A Schmidt, Marius Westenhoff, Sebastian |
author_sort | Claesson, Elin |
collection | PubMed |
description | Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, and bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation of the protein through structural changes. We present serial femtosecond X-ray crystallographic data of the chromophore-binding domains of a bacterial phytochrome at delay times of 1 ps and 10 ps after photoexcitation. The data reveal a twist of the D-ring, which leads to partial detachment of the chromophore from the protein. Unexpectedly, the conserved so-called pyrrole water is photodissociated from the chromophore, concomitant with movement of the A-ring and a key signaling aspartate. The changes are wired together by ultrafast backbone and water movements around the chromophore, channeling them into signal transduction towards the output domains. We suggest that the observed collective changes are important for the phytochrome photoresponse, explaining the earliest steps of how plants, fungi and bacteria sense red light. |
format | Online Article Text |
id | pubmed-7164956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-71649562020-04-20 The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser Claesson, Elin Wahlgren, Weixiao Yuan Takala, Heikki Pandey, Suraj Castillon, Leticia Kuznetsova, Valentyna Henry, Léocadie Panman, Matthijs Carrillo, Melissa Kübel, Joachim Nanekar, Rahul Isaksson, Linnéa Nimmrich, Amke Cellini, Andrea Morozov, Dmitry Maj, Michał Kurttila, Moona Bosman, Robert Nango, Eriko Tanaka, Rie Tanaka, Tomoyuki Fangjia, Luo Iwata, So Owada, Shigeki Moffat, Keith Groenhof, Gerrit Stojković, Emina A Ihalainen, Janne A Schmidt, Marius Westenhoff, Sebastian eLife Structural Biology and Molecular Biophysics Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, and bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation of the protein through structural changes. We present serial femtosecond X-ray crystallographic data of the chromophore-binding domains of a bacterial phytochrome at delay times of 1 ps and 10 ps after photoexcitation. The data reveal a twist of the D-ring, which leads to partial detachment of the chromophore from the protein. Unexpectedly, the conserved so-called pyrrole water is photodissociated from the chromophore, concomitant with movement of the A-ring and a key signaling aspartate. The changes are wired together by ultrafast backbone and water movements around the chromophore, channeling them into signal transduction towards the output domains. We suggest that the observed collective changes are important for the phytochrome photoresponse, explaining the earliest steps of how plants, fungi and bacteria sense red light. eLife Sciences Publications, Ltd 2020-03-31 /pmc/articles/PMC7164956/ /pubmed/32228856 http://dx.doi.org/10.7554/eLife.53514 Text en © 2020, Claesson et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Structural Biology and Molecular Biophysics Claesson, Elin Wahlgren, Weixiao Yuan Takala, Heikki Pandey, Suraj Castillon, Leticia Kuznetsova, Valentyna Henry, Léocadie Panman, Matthijs Carrillo, Melissa Kübel, Joachim Nanekar, Rahul Isaksson, Linnéa Nimmrich, Amke Cellini, Andrea Morozov, Dmitry Maj, Michał Kurttila, Moona Bosman, Robert Nango, Eriko Tanaka, Rie Tanaka, Tomoyuki Fangjia, Luo Iwata, So Owada, Shigeki Moffat, Keith Groenhof, Gerrit Stojković, Emina A Ihalainen, Janne A Schmidt, Marius Westenhoff, Sebastian The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser |
title | The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser |
title_full | The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser |
title_fullStr | The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser |
title_full_unstemmed | The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser |
title_short | The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser |
title_sort | primary structural photoresponse of phytochrome proteins captured by a femtosecond x-ray laser |
topic | Structural Biology and Molecular Biophysics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164956/ https://www.ncbi.nlm.nih.gov/pubmed/32228856 http://dx.doi.org/10.7554/eLife.53514 |
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