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First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community

Infection with SARS-CoV-2, the etiologic agent of the ongoing COVID-19 pandemic, is accompanied by the shedding of the virus in stool. Therefore, the quantification of SARS-CoV-2 in wastewater affords the ability to monitor the prevalence of infections among the population via wastewater-based epide...

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Autores principales: Ahmed, Warish, Angel, Nicola, Edson, Janette, Bibby, Kyle, Bivins, Aaron, O'Brien, Jake W., Choi, Phil M., Kitajima, Masaaki, Simpson, Stuart L., Li, Jiaying, Tscharke, Ben, Verhagen, Rory, Smith, Wendy J.M., Zaugg, Julian, Dierens, Leanne, Hugenholtz, Philip, Thomas, Kevin V., Mueller, Jochen F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165106/
https://www.ncbi.nlm.nih.gov/pubmed/32387778
http://dx.doi.org/10.1016/j.scitotenv.2020.138764
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author Ahmed, Warish
Angel, Nicola
Edson, Janette
Bibby, Kyle
Bivins, Aaron
O'Brien, Jake W.
Choi, Phil M.
Kitajima, Masaaki
Simpson, Stuart L.
Li, Jiaying
Tscharke, Ben
Verhagen, Rory
Smith, Wendy J.M.
Zaugg, Julian
Dierens, Leanne
Hugenholtz, Philip
Thomas, Kevin V.
Mueller, Jochen F.
author_facet Ahmed, Warish
Angel, Nicola
Edson, Janette
Bibby, Kyle
Bivins, Aaron
O'Brien, Jake W.
Choi, Phil M.
Kitajima, Masaaki
Simpson, Stuart L.
Li, Jiaying
Tscharke, Ben
Verhagen, Rory
Smith, Wendy J.M.
Zaugg, Julian
Dierens, Leanne
Hugenholtz, Philip
Thomas, Kevin V.
Mueller, Jochen F.
author_sort Ahmed, Warish
collection PubMed
description Infection with SARS-CoV-2, the etiologic agent of the ongoing COVID-19 pandemic, is accompanied by the shedding of the virus in stool. Therefore, the quantification of SARS-CoV-2 in wastewater affords the ability to monitor the prevalence of infections among the population via wastewater-based epidemiology (WBE). In the current work, SARS-CoV-2 RNA was concentrated from wastewater in a catchment in Australia and viral RNA copies were enumerated using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) resulting in two positive detections within a six day period from the same wastewater treatment plant (WWTP). The estimated viral RNA copy numbers observed in the wastewater were then used to estimate the number of infected individuals in the catchment via Monte Carlo simulation. Given the uncertainty and variation in the input parameters, the model estimated a median range of 171 to 1,090 infected persons in the catchment, which is in reasonable agreement with clinical observations. This work highlights the viability of WBE for monitoring infectious diseases, such as COVID-19, in communities. The work also draws attention to the need for further methodological and molecular assay validation for enveloped viruses in wastewater.
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spelling pubmed-71651062020-04-20 First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community Ahmed, Warish Angel, Nicola Edson, Janette Bibby, Kyle Bivins, Aaron O'Brien, Jake W. Choi, Phil M. Kitajima, Masaaki Simpson, Stuart L. Li, Jiaying Tscharke, Ben Verhagen, Rory Smith, Wendy J.M. Zaugg, Julian Dierens, Leanne Hugenholtz, Philip Thomas, Kevin V. Mueller, Jochen F. Sci Total Environ Article Infection with SARS-CoV-2, the etiologic agent of the ongoing COVID-19 pandemic, is accompanied by the shedding of the virus in stool. Therefore, the quantification of SARS-CoV-2 in wastewater affords the ability to monitor the prevalence of infections among the population via wastewater-based epidemiology (WBE). In the current work, SARS-CoV-2 RNA was concentrated from wastewater in a catchment in Australia and viral RNA copies were enumerated using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) resulting in two positive detections within a six day period from the same wastewater treatment plant (WWTP). The estimated viral RNA copy numbers observed in the wastewater were then used to estimate the number of infected individuals in the catchment via Monte Carlo simulation. Given the uncertainty and variation in the input parameters, the model estimated a median range of 171 to 1,090 infected persons in the catchment, which is in reasonable agreement with clinical observations. This work highlights the viability of WBE for monitoring infectious diseases, such as COVID-19, in communities. The work also draws attention to the need for further methodological and molecular assay validation for enveloped viruses in wastewater. Published by Elsevier B.V. 2020-08-01 2020-04-18 /pmc/articles/PMC7165106/ /pubmed/32387778 http://dx.doi.org/10.1016/j.scitotenv.2020.138764 Text en Crown Copyright © 2020 Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ahmed, Warish
Angel, Nicola
Edson, Janette
Bibby, Kyle
Bivins, Aaron
O'Brien, Jake W.
Choi, Phil M.
Kitajima, Masaaki
Simpson, Stuart L.
Li, Jiaying
Tscharke, Ben
Verhagen, Rory
Smith, Wendy J.M.
Zaugg, Julian
Dierens, Leanne
Hugenholtz, Philip
Thomas, Kevin V.
Mueller, Jochen F.
First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
title First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
title_full First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
title_fullStr First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
title_full_unstemmed First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
title_short First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
title_sort first confirmed detection of sars-cov-2 in untreated wastewater in australia: a proof of concept for the wastewater surveillance of covid-19 in the community
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165106/
https://www.ncbi.nlm.nih.gov/pubmed/32387778
http://dx.doi.org/10.1016/j.scitotenv.2020.138764
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