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Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent

Merkel cell carcinoma is a rare, lethal cancer histopathologically composed of cells showing similarity with mechanoreceptor Merkel cells. Merkel cell tumors manifest in two distinct forms. While a virus called Merkel cell polyomavirus is involved in the pathogenesis of one form of Merkel tumors, th...

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Autores principales: Arora, Reety, Rekhi, Bharat, Chandrani, Pratik, Krishna, Sudhir, Dutt, Amit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166130/
https://www.ncbi.nlm.nih.gov/pubmed/31600537
http://dx.doi.org/10.1016/j.micpath.2019.103778
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author Arora, Reety
Rekhi, Bharat
Chandrani, Pratik
Krishna, Sudhir
Dutt, Amit
author_facet Arora, Reety
Rekhi, Bharat
Chandrani, Pratik
Krishna, Sudhir
Dutt, Amit
author_sort Arora, Reety
collection PubMed
description Merkel cell carcinoma is a rare, lethal cancer histopathologically composed of cells showing similarity with mechanoreceptor Merkel cells. Merkel cell tumors manifest in two distinct forms. While a virus called Merkel cell polyomavirus is involved in the pathogenesis of one form of Merkel tumors, the other is driven by ultraviolet (UV)-linked mutations. In this study we investigated 18 cases, from the Indian population, of Merkel cell carcinoma for immunohistochemical (IHC) expression of Merkel cell polyomavirus (MCV) T antigen, including 12 cases tested by PCR, to identify viral etiopathology. We tested the tumors with two sensitive antibodies (CM2B4 and Ab3), targeting the viral large T antigen protein and with PCR primers targeting the N terminus of T antigen. Overall, we observed 38.8% (7/18) tumors displaying positive IHC expression of Merkel cell polyomavirus T antigen and 25% (3/12) tumors showing positive results, by both, immunohistochemistry and PCR. This constitutes the first report from India showing implication of MCV in Merkel cell carcinomas. Moreover, this is one of the larger series of Merkel cell carcinomas, tested for MCV, by both immunohistochemistry and PCR, in this part of the world. These results further indicate that a slightly more number of such cases in India are likely to be caused by UV-linked damage, as opposed to Merkel cell polyomavirus mediated tumorigenesis, which is definitely implicated in a subset of cases.
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spelling pubmed-71661302020-04-18 Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent Arora, Reety Rekhi, Bharat Chandrani, Pratik Krishna, Sudhir Dutt, Amit Microb Pathog Article Merkel cell carcinoma is a rare, lethal cancer histopathologically composed of cells showing similarity with mechanoreceptor Merkel cells. Merkel cell tumors manifest in two distinct forms. While a virus called Merkel cell polyomavirus is involved in the pathogenesis of one form of Merkel tumors, the other is driven by ultraviolet (UV)-linked mutations. In this study we investigated 18 cases, from the Indian population, of Merkel cell carcinoma for immunohistochemical (IHC) expression of Merkel cell polyomavirus (MCV) T antigen, including 12 cases tested by PCR, to identify viral etiopathology. We tested the tumors with two sensitive antibodies (CM2B4 and Ab3), targeting the viral large T antigen protein and with PCR primers targeting the N terminus of T antigen. Overall, we observed 38.8% (7/18) tumors displaying positive IHC expression of Merkel cell polyomavirus T antigen and 25% (3/12) tumors showing positive results, by both, immunohistochemistry and PCR. This constitutes the first report from India showing implication of MCV in Merkel cell carcinomas. Moreover, this is one of the larger series of Merkel cell carcinomas, tested for MCV, by both immunohistochemistry and PCR, in this part of the world. These results further indicate that a slightly more number of such cases in India are likely to be caused by UV-linked damage, as opposed to Merkel cell polyomavirus mediated tumorigenesis, which is definitely implicated in a subset of cases. 2019-12-01 2019-10-07 /pmc/articles/PMC7166130/ /pubmed/31600537 http://dx.doi.org/10.1016/j.micpath.2019.103778 Text en http://creativecommons.org/licenses/BY-NC-ND/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
spellingShingle Article
Arora, Reety
Rekhi, Bharat
Chandrani, Pratik
Krishna, Sudhir
Dutt, Amit
Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent
title Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent
title_full Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent
title_fullStr Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent
title_full_unstemmed Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent
title_short Merkel cell polyomavirus is implicated in a subset of Merkel cell carcinomas, in the Indian subcontinent
title_sort merkel cell polyomavirus is implicated in a subset of merkel cell carcinomas, in the indian subcontinent
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166130/
https://www.ncbi.nlm.nih.gov/pubmed/31600537
http://dx.doi.org/10.1016/j.micpath.2019.103778
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