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Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays

Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One...

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Autores principales: Tan, Li Kiang, Wong, Wing Yan, Yang, Hui Ting, Huber, Roland G., Bond, Peter J., Ng, Lee Ching, Maurer-Stroh, Sebastian, Hapuarachchi, Hapuarachchige Chanditha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167843/
https://www.ncbi.nlm.nih.gov/pubmed/31878299
http://dx.doi.org/10.3390/diagnostics10010011
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author Tan, Li Kiang
Wong, Wing Yan
Yang, Hui Ting
Huber, Roland G.
Bond, Peter J.
Ng, Lee Ching
Maurer-Stroh, Sebastian
Hapuarachchi, Hapuarachchige Chanditha
author_facet Tan, Li Kiang
Wong, Wing Yan
Yang, Hui Ting
Huber, Roland G.
Bond, Peter J.
Ng, Lee Ching
Maurer-Stroh, Sebastian
Hapuarachchi, Hapuarachchige Chanditha
author_sort Tan, Li Kiang
collection PubMed
description Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate.
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spelling pubmed-71678432020-04-21 Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays Tan, Li Kiang Wong, Wing Yan Yang, Hui Ting Huber, Roland G. Bond, Peter J. Ng, Lee Ching Maurer-Stroh, Sebastian Hapuarachchi, Hapuarachchige Chanditha Diagnostics (Basel) Article Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate. MDPI 2019-12-24 /pmc/articles/PMC7167843/ /pubmed/31878299 http://dx.doi.org/10.3390/diagnostics10010011 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tan, Li Kiang
Wong, Wing Yan
Yang, Hui Ting
Huber, Roland G.
Bond, Peter J.
Ng, Lee Ching
Maurer-Stroh, Sebastian
Hapuarachchi, Hapuarachchige Chanditha
Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
title Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
title_full Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
title_fullStr Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
title_full_unstemmed Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
title_short Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
title_sort flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167843/
https://www.ncbi.nlm.nih.gov/pubmed/31878299
http://dx.doi.org/10.3390/diagnostics10010011
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