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Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients

Chronic renal allograft dysfunction (CAD) is a major limiting factor of long-term graft survival. The hallmarks of progressive CAD are interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs are small, regulatory RNAs involved in many immunological processes. In particular, microRNA-21-5p (miR-2...

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Autores principales: Gniewkiewicz, Michal S., Paszkowska, Izabela, Gozdowska, Jolanta, Czerwinska, Katarzyna, Sadowska-Jakubowicz, Anna, Deborska-Materkowska, Dominika, Perkowska-Ptasinska, Agnieszka, Kosieradzki, Maciej, Durlik, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168003/
https://www.ncbi.nlm.nih.gov/pubmed/32092939
http://dx.doi.org/10.3390/diagnostics10020113
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author Gniewkiewicz, Michal S.
Paszkowska, Izabela
Gozdowska, Jolanta
Czerwinska, Katarzyna
Sadowska-Jakubowicz, Anna
Deborska-Materkowska, Dominika
Perkowska-Ptasinska, Agnieszka
Kosieradzki, Maciej
Durlik, Magdalena
author_facet Gniewkiewicz, Michal S.
Paszkowska, Izabela
Gozdowska, Jolanta
Czerwinska, Katarzyna
Sadowska-Jakubowicz, Anna
Deborska-Materkowska, Dominika
Perkowska-Ptasinska, Agnieszka
Kosieradzki, Maciej
Durlik, Magdalena
author_sort Gniewkiewicz, Michal S.
collection PubMed
description Chronic renal allograft dysfunction (CAD) is a major limiting factor of long-term graft survival. The hallmarks of progressive CAD are interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs are small, regulatory RNAs involved in many immunological processes. In particular, microRNA-21-5p (miR-21) is considered to be strongly associated with pathogenesis regarding tubulointerstitium. The aim of this study was to assess urinary miR-21 expression levels in the kidney transplant recipients and determine their application in the evaluation of IFTA and kidney allograft function. The expression levels of miR-21 were quantified in the urine of 31 kidney transplant recipients with biopsy-assessed IFTA (IFTA 0 + I: n = 17; IFTA II + III: n = 14) by real-time quantitative PCR. Urine samples were collected at the time of protocolar biopsies performed 1 or 2 years after kidney transplantation. MicroRNA-191-5p was used as reference gene. MiR-21 was significantly up-regulated in IFTA II + III group compared to IFTA 0 + I group (p = 0.003). MiR-21 correlated significantly with serum concentration of creatinine (r = 0.52, p = 0.003) and eGFR (r = −0.45; p = 0.01). ROC analysis determined the diagnostic value of miR-21 with an area under curve (AUC) of 0.80 (p = 0.0002), sensitivity of 0.86 and specificity of 0.71. miR-21 is associated with renal allograft dysfunction and IFTA. Therefore, it could be considered as a potential diagnostic, non-invasive biomarker for monitoring renal graft function.
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spelling pubmed-71680032020-04-21 Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients Gniewkiewicz, Michal S. Paszkowska, Izabela Gozdowska, Jolanta Czerwinska, Katarzyna Sadowska-Jakubowicz, Anna Deborska-Materkowska, Dominika Perkowska-Ptasinska, Agnieszka Kosieradzki, Maciej Durlik, Magdalena Diagnostics (Basel) Article Chronic renal allograft dysfunction (CAD) is a major limiting factor of long-term graft survival. The hallmarks of progressive CAD are interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs are small, regulatory RNAs involved in many immunological processes. In particular, microRNA-21-5p (miR-21) is considered to be strongly associated with pathogenesis regarding tubulointerstitium. The aim of this study was to assess urinary miR-21 expression levels in the kidney transplant recipients and determine their application in the evaluation of IFTA and kidney allograft function. The expression levels of miR-21 were quantified in the urine of 31 kidney transplant recipients with biopsy-assessed IFTA (IFTA 0 + I: n = 17; IFTA II + III: n = 14) by real-time quantitative PCR. Urine samples were collected at the time of protocolar biopsies performed 1 or 2 years after kidney transplantation. MicroRNA-191-5p was used as reference gene. MiR-21 was significantly up-regulated in IFTA II + III group compared to IFTA 0 + I group (p = 0.003). MiR-21 correlated significantly with serum concentration of creatinine (r = 0.52, p = 0.003) and eGFR (r = −0.45; p = 0.01). ROC analysis determined the diagnostic value of miR-21 with an area under curve (AUC) of 0.80 (p = 0.0002), sensitivity of 0.86 and specificity of 0.71. miR-21 is associated with renal allograft dysfunction and IFTA. Therefore, it could be considered as a potential diagnostic, non-invasive biomarker for monitoring renal graft function. MDPI 2020-02-19 /pmc/articles/PMC7168003/ /pubmed/32092939 http://dx.doi.org/10.3390/diagnostics10020113 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gniewkiewicz, Michal S.
Paszkowska, Izabela
Gozdowska, Jolanta
Czerwinska, Katarzyna
Sadowska-Jakubowicz, Anna
Deborska-Materkowska, Dominika
Perkowska-Ptasinska, Agnieszka
Kosieradzki, Maciej
Durlik, Magdalena
Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients
title Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients
title_full Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients
title_fullStr Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients
title_full_unstemmed Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients
title_short Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients
title_sort urinary microrna-21-5p as potential biomarker of interstitial fibrosis and tubular atrophy (ifta) in kidney transplant recipients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168003/
https://www.ncbi.nlm.nih.gov/pubmed/32092939
http://dx.doi.org/10.3390/diagnostics10020113
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