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Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus
Hepatitis C virus (HCV) infection is a major worldwide health problem which can cause chronic hepatitis, liver fibrosis and hepatocellular carcinoma (HCC). There is still no vaccine to prevent HCV infection. Currently, the clinical treatment of HCV infection mainly relies on the use of direct-acting...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168052/ https://www.ncbi.nlm.nih.gov/pubmed/31963532 http://dx.doi.org/10.3390/antibiotics9010033 |
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author | Cheng, Yuting Sun, Fang Li, Songryong Gao, Minjun Wang, Luyao Sarhan, Moustafa Abdel-Rahman, Mohamed A. Li, Wenxin Kwok, Hang Fai Wu, Yingliang Cao, Zhijian |
author_facet | Cheng, Yuting Sun, Fang Li, Songryong Gao, Minjun Wang, Luyao Sarhan, Moustafa Abdel-Rahman, Mohamed A. Li, Wenxin Kwok, Hang Fai Wu, Yingliang Cao, Zhijian |
author_sort | Cheng, Yuting |
collection | PubMed |
description | Hepatitis C virus (HCV) infection is a major worldwide health problem which can cause chronic hepatitis, liver fibrosis and hepatocellular carcinoma (HCC). There is still no vaccine to prevent HCV infection. Currently, the clinical treatment of HCV infection mainly relies on the use of direct-acting antivirals (DAAs) which are expensive and have side effects. Here, BmKDfsin3, a scorpion defensin from the venom of Mesobuthus martensii Karsch, is found to dose-dependently inhibit HCV infection at noncytotoxic concentrations and affect viral attachment and post-entry in HCV life cycle. Further experimental results show that BmKDfsin3 not only suppresses p38 mitogen-activated protein kinase (MAPK) activation of HCV-infected Huh7.5.1 cells, but also inhibits p38 activation of Huh7.5.1 cells stimulated by tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or lipopolysaccharide (LPS). BmKDfsin3 is also revealed to enter into cells. Using an upstream MyD88 dimerization inhibitor ST2345 or kinase IRAK-1/4 inhibitor I, the inhibition of p38 activation represses HCV replication in vitro. Taken together, a scorpion defensin BmKDfsin3 inhibits HCV replication, related to regulated p38 MAPK activation. |
format | Online Article Text |
id | pubmed-7168052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71680522020-04-21 Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus Cheng, Yuting Sun, Fang Li, Songryong Gao, Minjun Wang, Luyao Sarhan, Moustafa Abdel-Rahman, Mohamed A. Li, Wenxin Kwok, Hang Fai Wu, Yingliang Cao, Zhijian Antibiotics (Basel) Article Hepatitis C virus (HCV) infection is a major worldwide health problem which can cause chronic hepatitis, liver fibrosis and hepatocellular carcinoma (HCC). There is still no vaccine to prevent HCV infection. Currently, the clinical treatment of HCV infection mainly relies on the use of direct-acting antivirals (DAAs) which are expensive and have side effects. Here, BmKDfsin3, a scorpion defensin from the venom of Mesobuthus martensii Karsch, is found to dose-dependently inhibit HCV infection at noncytotoxic concentrations and affect viral attachment and post-entry in HCV life cycle. Further experimental results show that BmKDfsin3 not only suppresses p38 mitogen-activated protein kinase (MAPK) activation of HCV-infected Huh7.5.1 cells, but also inhibits p38 activation of Huh7.5.1 cells stimulated by tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or lipopolysaccharide (LPS). BmKDfsin3 is also revealed to enter into cells. Using an upstream MyD88 dimerization inhibitor ST2345 or kinase IRAK-1/4 inhibitor I, the inhibition of p38 activation represses HCV replication in vitro. Taken together, a scorpion defensin BmKDfsin3 inhibits HCV replication, related to regulated p38 MAPK activation. MDPI 2020-01-17 /pmc/articles/PMC7168052/ /pubmed/31963532 http://dx.doi.org/10.3390/antibiotics9010033 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cheng, Yuting Sun, Fang Li, Songryong Gao, Minjun Wang, Luyao Sarhan, Moustafa Abdel-Rahman, Mohamed A. Li, Wenxin Kwok, Hang Fai Wu, Yingliang Cao, Zhijian Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus |
title | Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus |
title_full | Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus |
title_fullStr | Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus |
title_full_unstemmed | Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus |
title_short | Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus |
title_sort | inhibitory activity of a scorpion defensin bmkdfsin3 against hepatitis c virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168052/ https://www.ncbi.nlm.nih.gov/pubmed/31963532 http://dx.doi.org/10.3390/antibiotics9010033 |
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