Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia

Immune biomarkers can stratify mortality risk in staphylococcal bacteremia. Microbial biomarkers may provide more consistent signals during early infection. We demonstrate that in ST45/USA600 bacteremia, bacterial membrane vesicle production in vitro predicts clinical mortality (773 vs. 116 RFU, sur...

Descripción completa

Detalles Bibliográficos
Autores principales: Dey, Somrita, Gudipati, Smitha, Giuliano, Christopher, Zervos, Marcus J., Monk, Jonathan M., Szubin, Richard, Jorgensen, Sarah C. J., Sakoulas, George, Berti, Andrew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168145/
https://www.ncbi.nlm.nih.gov/pubmed/31861446
http://dx.doi.org/10.3390/antibiotics9010002
_version_ 1783523619861692416
author Dey, Somrita
Gudipati, Smitha
Giuliano, Christopher
Zervos, Marcus J.
Monk, Jonathan M.
Szubin, Richard
Jorgensen, Sarah C. J.
Sakoulas, George
Berti, Andrew D.
author_facet Dey, Somrita
Gudipati, Smitha
Giuliano, Christopher
Zervos, Marcus J.
Monk, Jonathan M.
Szubin, Richard
Jorgensen, Sarah C. J.
Sakoulas, George
Berti, Andrew D.
author_sort Dey, Somrita
collection PubMed
description Immune biomarkers can stratify mortality risk in staphylococcal bacteremia. Microbial biomarkers may provide more consistent signals during early infection. We demonstrate that in ST45/USA600 bacteremia, bacterial membrane vesicle production in vitro predicts clinical mortality (773 vs. 116 RFU, survivors vs. decedents, p < 0.0001). Using a threshold of 301 relative fluorescence units (RFU), the sensitivity and specificity of the membrane vesicles to predict mortality are 78% and 90%, respectively. This platform is facile, scalable and can be integrated into clinical microbiology lab workflows.
format Online
Article
Text
id pubmed-7168145
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71681452020-04-21 Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia Dey, Somrita Gudipati, Smitha Giuliano, Christopher Zervos, Marcus J. Monk, Jonathan M. Szubin, Richard Jorgensen, Sarah C. J. Sakoulas, George Berti, Andrew D. Antibiotics (Basel) Article Immune biomarkers can stratify mortality risk in staphylococcal bacteremia. Microbial biomarkers may provide more consistent signals during early infection. We demonstrate that in ST45/USA600 bacteremia, bacterial membrane vesicle production in vitro predicts clinical mortality (773 vs. 116 RFU, survivors vs. decedents, p < 0.0001). Using a threshold of 301 relative fluorescence units (RFU), the sensitivity and specificity of the membrane vesicles to predict mortality are 78% and 90%, respectively. This platform is facile, scalable and can be integrated into clinical microbiology lab workflows. MDPI 2019-12-18 /pmc/articles/PMC7168145/ /pubmed/31861446 http://dx.doi.org/10.3390/antibiotics9010002 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dey, Somrita
Gudipati, Smitha
Giuliano, Christopher
Zervos, Marcus J.
Monk, Jonathan M.
Szubin, Richard
Jorgensen, Sarah C. J.
Sakoulas, George
Berti, Andrew D.
Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia
title Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia
title_full Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia
title_fullStr Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia
title_full_unstemmed Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia
title_short Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia
title_sort reduced production of bacterial membrane vesicles predicts mortality in st45/usa600 methicillin-resistant staphylococcus aureus bacteremia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168145/
https://www.ncbi.nlm.nih.gov/pubmed/31861446
http://dx.doi.org/10.3390/antibiotics9010002
work_keys_str_mv AT deysomrita reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT gudipatismitha reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT giulianochristopher reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT zervosmarcusj reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT monkjonathanm reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT szubinrichard reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT jorgensensarahcj reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT sakoulasgeorge reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia
AT bertiandrewd reducedproductionofbacterialmembranevesiclespredictsmortalityinst45usa600methicillinresistantstaphylococcusaureusbacteremia

Ejemplares similares