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Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and cu...

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Autores principales: Prasad Pawar, Rajendra, Mishra, Pooja, Durgbanshi, Abhilasha, Bose, Devasish, Albiol-Chiva, Jaume, Peris-Vicente, Juan, García-Ferrer, Daniel, Esteve-Romero, Josep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168181/
https://www.ncbi.nlm.nih.gov/pubmed/32075155
http://dx.doi.org/10.3390/antibiotics9020086
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author Prasad Pawar, Rajendra
Mishra, Pooja
Durgbanshi, Abhilasha
Bose, Devasish
Albiol-Chiva, Jaume
Peris-Vicente, Juan
García-Ferrer, Daniel
Esteve-Romero, Josep
author_facet Prasad Pawar, Rajendra
Mishra, Pooja
Durgbanshi, Abhilasha
Bose, Devasish
Albiol-Chiva, Jaume
Peris-Vicente, Juan
García-Ferrer, Daniel
Esteve-Romero, Josep
author_sort Prasad Pawar, Rajendra
collection PubMed
description Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)–6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25–50 ppm), sensitivity (limit of detection 0.1–0.2 ppm; limit of quantification, 0.3–0.6 ppm), trueness (92.5–102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control.
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spelling pubmed-71681812020-04-21 Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals Prasad Pawar, Rajendra Mishra, Pooja Durgbanshi, Abhilasha Bose, Devasish Albiol-Chiva, Jaume Peris-Vicente, Juan García-Ferrer, Daniel Esteve-Romero, Josep Antibiotics (Basel) Article Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)–6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25–50 ppm), sensitivity (limit of detection 0.1–0.2 ppm; limit of quantification, 0.3–0.6 ppm), trueness (92.5–102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control. MDPI 2020-02-14 /pmc/articles/PMC7168181/ /pubmed/32075155 http://dx.doi.org/10.3390/antibiotics9020086 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Prasad Pawar, Rajendra
Mishra, Pooja
Durgbanshi, Abhilasha
Bose, Devasish
Albiol-Chiva, Jaume
Peris-Vicente, Juan
García-Ferrer, Daniel
Esteve-Romero, Josep
Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals
title Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals
title_full Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals
title_fullStr Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals
title_full_unstemmed Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals
title_short Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals
title_sort use of micellar liquid chromatography to determine mebendazole in dairy products and breeding waste from bovine animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168181/
https://www.ncbi.nlm.nih.gov/pubmed/32075155
http://dx.doi.org/10.3390/antibiotics9020086
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