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Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea

Respiratory diseases cause significant economic losses (especially in the horse racing industry). The present study describes the detection and genetic characteristics of equine herpesvirus (EHV) from a total of 1497 samples from clinically healthy horses in Korea, including 926 blood samples, 187 l...

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Autores principales: Seo, Min-Goo, Ouh, In-Ohk, Lee, Sang Kyu, Lee, Jong-Seok, Kwon, Oh-Deog, Kwak, Dongmi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168308/
https://www.ncbi.nlm.nih.gov/pubmed/32053974
http://dx.doi.org/10.3390/pathogens9020110
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author Seo, Min-Goo
Ouh, In-Ohk
Lee, Sang Kyu
Lee, Jong-Seok
Kwon, Oh-Deog
Kwak, Dongmi
author_facet Seo, Min-Goo
Ouh, In-Ohk
Lee, Sang Kyu
Lee, Jong-Seok
Kwon, Oh-Deog
Kwak, Dongmi
author_sort Seo, Min-Goo
collection PubMed
description Respiratory diseases cause significant economic losses (especially in the horse racing industry). The present study describes the detection and genetic characteristics of equine herpesvirus (EHV) from a total of 1497 samples from clinically healthy horses in Korea, including 926 blood samples, 187 lung tissues, and 384 nasal swabs. EHV-2 and EHV-5 were detected in 386 (41.7%; 95% CI: 38.5–44.9) and 201 (21.7%; 95% CI: 19.1–24.4) blood samples, respectively, and in 25 (13.4%; 95% CI: 8.5–18.2) and 35 (18.7%; 95% CI: 13.1–24.3) lung tissues, respectively. EHV-1 and EHV-4 were not detected in either blood or lung tissues. EHV-1, EHV-2, and EHV-5 were detected in 46 (12.0%; 95% CI: 8.7–15.2), 21 (5.5%; 95% CI: 3.2–7.7), and 43 (11.2%; 95% CI: 8.0–14.4) nasal swabs, respectively. EHV-4 was not detected in nasal swabs. Co-infection with EHV-2 and EHV-5 was detected in 11.6% (107/926) of the blood samples and 6.4% (12/187) of lung tissues. In nasal swabs, co-infection with EHV-1, EHV-2, and EHV-5 was detected in 0.8% (3/384) of samples. Phylogenetic analysis of the glycoprotein B gene showed that EHV-1, EHV-2, and EHV-5 strains demonstrated significant genetic diversity in Korea, with a nucleotide sequence identity among them that ranged from 95.7% to 100% for EHV-1, 96.2–100% for EHV-2, and 93.8–99.3% for EHV-5. These results are the first phylogenetic analyses of EHV-1 in Korea in nasal swabs from a nationwide population of clinically healthy horses. Both EHV-2 and EHV-5 from blood, lung tissues, and nasal swabs were also detected.
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spelling pubmed-71683082020-04-22 Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea Seo, Min-Goo Ouh, In-Ohk Lee, Sang Kyu Lee, Jong-Seok Kwon, Oh-Deog Kwak, Dongmi Pathogens Article Respiratory diseases cause significant economic losses (especially in the horse racing industry). The present study describes the detection and genetic characteristics of equine herpesvirus (EHV) from a total of 1497 samples from clinically healthy horses in Korea, including 926 blood samples, 187 lung tissues, and 384 nasal swabs. EHV-2 and EHV-5 were detected in 386 (41.7%; 95% CI: 38.5–44.9) and 201 (21.7%; 95% CI: 19.1–24.4) blood samples, respectively, and in 25 (13.4%; 95% CI: 8.5–18.2) and 35 (18.7%; 95% CI: 13.1–24.3) lung tissues, respectively. EHV-1 and EHV-4 were not detected in either blood or lung tissues. EHV-1, EHV-2, and EHV-5 were detected in 46 (12.0%; 95% CI: 8.7–15.2), 21 (5.5%; 95% CI: 3.2–7.7), and 43 (11.2%; 95% CI: 8.0–14.4) nasal swabs, respectively. EHV-4 was not detected in nasal swabs. Co-infection with EHV-2 and EHV-5 was detected in 11.6% (107/926) of the blood samples and 6.4% (12/187) of lung tissues. In nasal swabs, co-infection with EHV-1, EHV-2, and EHV-5 was detected in 0.8% (3/384) of samples. Phylogenetic analysis of the glycoprotein B gene showed that EHV-1, EHV-2, and EHV-5 strains demonstrated significant genetic diversity in Korea, with a nucleotide sequence identity among them that ranged from 95.7% to 100% for EHV-1, 96.2–100% for EHV-2, and 93.8–99.3% for EHV-5. These results are the first phylogenetic analyses of EHV-1 in Korea in nasal swabs from a nationwide population of clinically healthy horses. Both EHV-2 and EHV-5 from blood, lung tissues, and nasal swabs were also detected. MDPI 2020-02-11 /pmc/articles/PMC7168308/ /pubmed/32053974 http://dx.doi.org/10.3390/pathogens9020110 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seo, Min-Goo
Ouh, In-Ohk
Lee, Sang Kyu
Lee, Jong-Seok
Kwon, Oh-Deog
Kwak, Dongmi
Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea
title Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea
title_full Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea
title_fullStr Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea
title_full_unstemmed Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea
title_short Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea
title_sort molecular detection and genetic characteristics of equine herpesvirus in korea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168308/
https://www.ncbi.nlm.nih.gov/pubmed/32053974
http://dx.doi.org/10.3390/pathogens9020110
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