Cargando…

Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231

Metabolic reprogramming in tumor cells is considered one of the hallmarks of cancer. Many studies have been carried out in order to elucidate the effects of tumor cell metabolism on invasion and tumor progression. However, little is known about the immediate substrate preference in tumor cells. In t...

Descripción completa

Detalles Bibliográficos
Autores principales: Ocaña, Mª Carmen, Martínez-Poveda, Beatriz, Quesada, Ana R., Medina, Miguel Ángel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168317/
https://www.ncbi.nlm.nih.gov/pubmed/31936882
http://dx.doi.org/10.3390/biology9010016
_version_ 1783523660735184896
author Ocaña, Mª Carmen
Martínez-Poveda, Beatriz
Quesada, Ana R.
Medina, Miguel Ángel
author_facet Ocaña, Mª Carmen
Martínez-Poveda, Beatriz
Quesada, Ana R.
Medina, Miguel Ángel
author_sort Ocaña, Mª Carmen
collection PubMed
description Metabolic reprogramming in tumor cells is considered one of the hallmarks of cancer. Many studies have been carried out in order to elucidate the effects of tumor cell metabolism on invasion and tumor progression. However, little is known about the immediate substrate preference in tumor cells. In this work, we wanted to study this short-time preference using the highly invasive, hormone independent breast cancer cell line MDA-MB-231. By means of Seahorse and uptake experiments, our results point to a preference for glucose. However, although both glucose and glutamine are required for tumor cell proliferation, MDA-MB-231 cells can survive two days in the absence of glucose, but not in the absence of glutamine. On the other hand, the presence of glucose increased palmitate uptake in this cell line, which accumulates in the cytosol instead of going to the plasma membrane. In order to exert this effect, glucose needs to be converted to glycerol-3 phosphate, leading to palmitate metabolism through lipid synthesis, most likely to the synthesis of triacylglycerides. The effect of glucose on the palmitate uptake was also found in other triple-negative, invasive breast cancer cell lines, but not in the non-invasive ones. The results presented in this work suggest an important and specific role of glucose in lipid biosynthesis in triple-negative breast cancer.
format Online
Article
Text
id pubmed-7168317
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71683172020-04-22 Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231 Ocaña, Mª Carmen Martínez-Poveda, Beatriz Quesada, Ana R. Medina, Miguel Ángel Biology (Basel) Article Metabolic reprogramming in tumor cells is considered one of the hallmarks of cancer. Many studies have been carried out in order to elucidate the effects of tumor cell metabolism on invasion and tumor progression. However, little is known about the immediate substrate preference in tumor cells. In this work, we wanted to study this short-time preference using the highly invasive, hormone independent breast cancer cell line MDA-MB-231. By means of Seahorse and uptake experiments, our results point to a preference for glucose. However, although both glucose and glutamine are required for tumor cell proliferation, MDA-MB-231 cells can survive two days in the absence of glucose, but not in the absence of glutamine. On the other hand, the presence of glucose increased palmitate uptake in this cell line, which accumulates in the cytosol instead of going to the plasma membrane. In order to exert this effect, glucose needs to be converted to glycerol-3 phosphate, leading to palmitate metabolism through lipid synthesis, most likely to the synthesis of triacylglycerides. The effect of glucose on the palmitate uptake was also found in other triple-negative, invasive breast cancer cell lines, but not in the non-invasive ones. The results presented in this work suggest an important and specific role of glucose in lipid biosynthesis in triple-negative breast cancer. MDPI 2020-01-10 /pmc/articles/PMC7168317/ /pubmed/31936882 http://dx.doi.org/10.3390/biology9010016 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ocaña, Mª Carmen
Martínez-Poveda, Beatriz
Quesada, Ana R.
Medina, Miguel Ángel
Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231
title Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231
title_full Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231
title_fullStr Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231
title_full_unstemmed Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231
title_short Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231
title_sort glucose favors lipid anabolic metabolism in the invasive breast cancer cell line mda-mb-231
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168317/
https://www.ncbi.nlm.nih.gov/pubmed/31936882
http://dx.doi.org/10.3390/biology9010016
work_keys_str_mv AT ocanamacarmen glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231
AT martinezpovedabeatriz glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231
AT quesadaanar glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231
AT medinamiguelangel glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231