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Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
Acanthamoeba is a genus of free-living amoebae widely distributed in nature, associated with the development of encephalitis and keratitis. Despite the fact that it is common to find genotype T5 in environmental samples, only a few cases have been associated with clinical cases in humans. The wide d...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168589/ https://www.ncbi.nlm.nih.gov/pubmed/32098034 http://dx.doi.org/10.3390/pathogens9020144 |
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author | Retana Moreira, Lissette Vargas Ramírez, Daniel Linares, Fátima Prescilla Ledezma, Alexa Vaglio Garro, Annette Osuna, Antonio Lorenzo Morales, Jacob Abrahams Sandí, Elizabeth |
author_facet | Retana Moreira, Lissette Vargas Ramírez, Daniel Linares, Fátima Prescilla Ledezma, Alexa Vaglio Garro, Annette Osuna, Antonio Lorenzo Morales, Jacob Abrahams Sandí, Elizabeth |
author_sort | Retana Moreira, Lissette |
collection | PubMed |
description | Acanthamoeba is a genus of free-living amoebae widely distributed in nature, associated with the development of encephalitis and keratitis. Despite the fact that it is common to find genotype T5 in environmental samples, only a few cases have been associated with clinical cases in humans. The wide distribution of Acanthamoeba, the characteristic of being amphizoic and the severity of the disease motivate researchers to focus on the isolation of these organisms, but also in demonstrating direct and indirect factors that could indicate a possible pathogenic potential. Here, we performed the characterization of the pathogenic potential of an Acanthamoeba T5 isolate collected from a water source in a hospital. Osmo- and thermotolerance, the secretion of proteases and the effect of trophozoites over cell monolayers were analyzed by different methodologies. Additionally, we confirm the secretion of extracellular vesicles (EVs) of this isolate incubated at two different temperatures, and the presence of serine and cysteine proteases in these vesicles. Finally, using atomic force microscopy, we determined some nanomechanical properties of the secreted vesicles and found a higher value of adhesion in the EVs obtained at 37 °C, which could have implications in the parasite´s survival and damaging potential in two different biological environments. |
format | Online Article Text |
id | pubmed-7168589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71685892020-04-22 Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles Retana Moreira, Lissette Vargas Ramírez, Daniel Linares, Fátima Prescilla Ledezma, Alexa Vaglio Garro, Annette Osuna, Antonio Lorenzo Morales, Jacob Abrahams Sandí, Elizabeth Pathogens Article Acanthamoeba is a genus of free-living amoebae widely distributed in nature, associated with the development of encephalitis and keratitis. Despite the fact that it is common to find genotype T5 in environmental samples, only a few cases have been associated with clinical cases in humans. The wide distribution of Acanthamoeba, the characteristic of being amphizoic and the severity of the disease motivate researchers to focus on the isolation of these organisms, but also in demonstrating direct and indirect factors that could indicate a possible pathogenic potential. Here, we performed the characterization of the pathogenic potential of an Acanthamoeba T5 isolate collected from a water source in a hospital. Osmo- and thermotolerance, the secretion of proteases and the effect of trophozoites over cell monolayers were analyzed by different methodologies. Additionally, we confirm the secretion of extracellular vesicles (EVs) of this isolate incubated at two different temperatures, and the presence of serine and cysteine proteases in these vesicles. Finally, using atomic force microscopy, we determined some nanomechanical properties of the secreted vesicles and found a higher value of adhesion in the EVs obtained at 37 °C, which could have implications in the parasite´s survival and damaging potential in two different biological environments. MDPI 2020-02-21 /pmc/articles/PMC7168589/ /pubmed/32098034 http://dx.doi.org/10.3390/pathogens9020144 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Retana Moreira, Lissette Vargas Ramírez, Daniel Linares, Fátima Prescilla Ledezma, Alexa Vaglio Garro, Annette Osuna, Antonio Lorenzo Morales, Jacob Abrahams Sandí, Elizabeth Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles |
title | Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles |
title_full | Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles |
title_fullStr | Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles |
title_full_unstemmed | Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles |
title_short | Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles |
title_sort | isolation of acanthamoeba t5 from water: characterization of its pathogenic potential, including the production of extracellular vesicles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168589/ https://www.ncbi.nlm.nih.gov/pubmed/32098034 http://dx.doi.org/10.3390/pathogens9020144 |
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