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Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles

Acanthamoeba is a genus of free-living amoebae widely distributed in nature, associated with the development of encephalitis and keratitis. Despite the fact that it is common to find genotype T5 in environmental samples, only a few cases have been associated with clinical cases in humans. The wide d...

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Autores principales: Retana Moreira, Lissette, Vargas Ramírez, Daniel, Linares, Fátima, Prescilla Ledezma, Alexa, Vaglio Garro, Annette, Osuna, Antonio, Lorenzo Morales, Jacob, Abrahams Sandí, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168589/
https://www.ncbi.nlm.nih.gov/pubmed/32098034
http://dx.doi.org/10.3390/pathogens9020144
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author Retana Moreira, Lissette
Vargas Ramírez, Daniel
Linares, Fátima
Prescilla Ledezma, Alexa
Vaglio Garro, Annette
Osuna, Antonio
Lorenzo Morales, Jacob
Abrahams Sandí, Elizabeth
author_facet Retana Moreira, Lissette
Vargas Ramírez, Daniel
Linares, Fátima
Prescilla Ledezma, Alexa
Vaglio Garro, Annette
Osuna, Antonio
Lorenzo Morales, Jacob
Abrahams Sandí, Elizabeth
author_sort Retana Moreira, Lissette
collection PubMed
description Acanthamoeba is a genus of free-living amoebae widely distributed in nature, associated with the development of encephalitis and keratitis. Despite the fact that it is common to find genotype T5 in environmental samples, only a few cases have been associated with clinical cases in humans. The wide distribution of Acanthamoeba, the characteristic of being amphizoic and the severity of the disease motivate researchers to focus on the isolation of these organisms, but also in demonstrating direct and indirect factors that could indicate a possible pathogenic potential. Here, we performed the characterization of the pathogenic potential of an Acanthamoeba T5 isolate collected from a water source in a hospital. Osmo- and thermotolerance, the secretion of proteases and the effect of trophozoites over cell monolayers were analyzed by different methodologies. Additionally, we confirm the secretion of extracellular vesicles (EVs) of this isolate incubated at two different temperatures, and the presence of serine and cysteine proteases in these vesicles. Finally, using atomic force microscopy, we determined some nanomechanical properties of the secreted vesicles and found a higher value of adhesion in the EVs obtained at 37 °C, which could have implications in the parasite´s survival and damaging potential in two different biological environments.
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spelling pubmed-71685892020-04-22 Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles Retana Moreira, Lissette Vargas Ramírez, Daniel Linares, Fátima Prescilla Ledezma, Alexa Vaglio Garro, Annette Osuna, Antonio Lorenzo Morales, Jacob Abrahams Sandí, Elizabeth Pathogens Article Acanthamoeba is a genus of free-living amoebae widely distributed in nature, associated with the development of encephalitis and keratitis. Despite the fact that it is common to find genotype T5 in environmental samples, only a few cases have been associated with clinical cases in humans. The wide distribution of Acanthamoeba, the characteristic of being amphizoic and the severity of the disease motivate researchers to focus on the isolation of these organisms, but also in demonstrating direct and indirect factors that could indicate a possible pathogenic potential. Here, we performed the characterization of the pathogenic potential of an Acanthamoeba T5 isolate collected from a water source in a hospital. Osmo- and thermotolerance, the secretion of proteases and the effect of trophozoites over cell monolayers were analyzed by different methodologies. Additionally, we confirm the secretion of extracellular vesicles (EVs) of this isolate incubated at two different temperatures, and the presence of serine and cysteine proteases in these vesicles. Finally, using atomic force microscopy, we determined some nanomechanical properties of the secreted vesicles and found a higher value of adhesion in the EVs obtained at 37 °C, which could have implications in the parasite´s survival and damaging potential in two different biological environments. MDPI 2020-02-21 /pmc/articles/PMC7168589/ /pubmed/32098034 http://dx.doi.org/10.3390/pathogens9020144 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Retana Moreira, Lissette
Vargas Ramírez, Daniel
Linares, Fátima
Prescilla Ledezma, Alexa
Vaglio Garro, Annette
Osuna, Antonio
Lorenzo Morales, Jacob
Abrahams Sandí, Elizabeth
Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
title Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
title_full Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
title_fullStr Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
title_full_unstemmed Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
title_short Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
title_sort isolation of acanthamoeba t5 from water: characterization of its pathogenic potential, including the production of extracellular vesicles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168589/
https://www.ncbi.nlm.nih.gov/pubmed/32098034
http://dx.doi.org/10.3390/pathogens9020144
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