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New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India

Members of the genus Curvularia are melanin-producing dematiaceous fungi of increasing clinical importance as causal agents of both local and invasive infections. This study contributes to the taxonomical and clinical knowledge of this genus by describing two new Curvularia species based on isolates...

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Autores principales: Kiss, Noémi, Homa, Mónika, Manikandan, Palanisamy, Mythili, Arumugam, Krizsán, Krisztina, Revathi, Rajaraman, Varga, Mónika, Papp, Tamás, Vágvölgyi, Csaba, Kredics, László, Kocsubé, Sándor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168623/
https://www.ncbi.nlm.nih.gov/pubmed/31861831
http://dx.doi.org/10.3390/pathogens9010009
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author Kiss, Noémi
Homa, Mónika
Manikandan, Palanisamy
Mythili, Arumugam
Krizsán, Krisztina
Revathi, Rajaraman
Varga, Mónika
Papp, Tamás
Vágvölgyi, Csaba
Kredics, László
Kocsubé, Sándor
author_facet Kiss, Noémi
Homa, Mónika
Manikandan, Palanisamy
Mythili, Arumugam
Krizsán, Krisztina
Revathi, Rajaraman
Varga, Mónika
Papp, Tamás
Vágvölgyi, Csaba
Kredics, László
Kocsubé, Sándor
author_sort Kiss, Noémi
collection PubMed
description Members of the genus Curvularia are melanin-producing dematiaceous fungi of increasing clinical importance as causal agents of both local and invasive infections. This study contributes to the taxonomical and clinical knowledge of this genus by describing two new Curvularia species based on isolates from corneal scrapings of South Indian fungal keratitis patients. The phylogeny of the genus was updated based on three phylogenetic markers: the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster as well as fragments of the glyceraldehyde-3-phosphate dehydrogenase (gpdh) and translation elongation factor 1-α (tef1α) genes. The maximum likelihood phylogenetic tree constructed from the alignment of the three concatenated loci revealed that the examined isolates are representing two new, yet undescribed, Curvularia species. Examination of colony and microscopic morphology revealed differences between the two species as well as between the new species and their close relatives. The new species were formally described as Curvularia tamilnaduensis N. Kiss & S. Kocsubé sp. nov. and Curvularia coimbatorensis N. Kiss & S. Kocsubé sp. nov. Antifungal susceptibility testing by the broth microdilution method of CLSI (Clinical & Laboratory Standards Institute) revealed that the type strain of C. coimbatorensis is less susceptible to a series of antifungals than the C. tamilnaduensis strains.
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spelling pubmed-71686232020-04-22 New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India Kiss, Noémi Homa, Mónika Manikandan, Palanisamy Mythili, Arumugam Krizsán, Krisztina Revathi, Rajaraman Varga, Mónika Papp, Tamás Vágvölgyi, Csaba Kredics, László Kocsubé, Sándor Pathogens Article Members of the genus Curvularia are melanin-producing dematiaceous fungi of increasing clinical importance as causal agents of both local and invasive infections. This study contributes to the taxonomical and clinical knowledge of this genus by describing two new Curvularia species based on isolates from corneal scrapings of South Indian fungal keratitis patients. The phylogeny of the genus was updated based on three phylogenetic markers: the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster as well as fragments of the glyceraldehyde-3-phosphate dehydrogenase (gpdh) and translation elongation factor 1-α (tef1α) genes. The maximum likelihood phylogenetic tree constructed from the alignment of the three concatenated loci revealed that the examined isolates are representing two new, yet undescribed, Curvularia species. Examination of colony and microscopic morphology revealed differences between the two species as well as between the new species and their close relatives. The new species were formally described as Curvularia tamilnaduensis N. Kiss & S. Kocsubé sp. nov. and Curvularia coimbatorensis N. Kiss & S. Kocsubé sp. nov. Antifungal susceptibility testing by the broth microdilution method of CLSI (Clinical & Laboratory Standards Institute) revealed that the type strain of C. coimbatorensis is less susceptible to a series of antifungals than the C. tamilnaduensis strains. MDPI 2019-12-20 /pmc/articles/PMC7168623/ /pubmed/31861831 http://dx.doi.org/10.3390/pathogens9010009 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kiss, Noémi
Homa, Mónika
Manikandan, Palanisamy
Mythili, Arumugam
Krizsán, Krisztina
Revathi, Rajaraman
Varga, Mónika
Papp, Tamás
Vágvölgyi, Csaba
Kredics, László
Kocsubé, Sándor
New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India
title New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India
title_full New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India
title_fullStr New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India
title_full_unstemmed New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India
title_short New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India
title_sort new species of the genus curvularia: c. tamilnaduensis and c. coimbatorensis from fungal keratitis cases in south india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168623/
https://www.ncbi.nlm.nih.gov/pubmed/31861831
http://dx.doi.org/10.3390/pathogens9010009
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