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Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species
Vulvovaginal candidiasis is a common fungal infection in women. In this study, Platonia insignis hydroalcoholic extract (PiHE) and its fractions were evaluated for antifungal and antivirulence activities against vaginal Candida species. Dichloromethane (DCMF) and ethyl acetate fractions (EAF) obtain...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168675/ https://www.ncbi.nlm.nih.gov/pubmed/32013047 http://dx.doi.org/10.3390/pathogens9020084 |
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author | da Silva, Anderson França da Rocha, Cláudia Quintino da Silva, Luís Cláudio Nascimento Carvalho Júnior, Alexsander Rodrigues Mendes, Iven Neylla Farias Vale de Araruna, Andrea Borges Motta, Elizangela Pestana Silva, Rayssa de Sousa Campos, Carmem Duarte Lima Farias, Josivan Regis Oliveira, Aluísio da Silva Silva, Douglas Henrique dos Santos Nascimento, Flávia Raquel F Guerra, Rosane Nassar Meireles Monteiro, Cristina Andrade |
author_facet | da Silva, Anderson França da Rocha, Cláudia Quintino da Silva, Luís Cláudio Nascimento Carvalho Júnior, Alexsander Rodrigues Mendes, Iven Neylla Farias Vale de Araruna, Andrea Borges Motta, Elizangela Pestana Silva, Rayssa de Sousa Campos, Carmem Duarte Lima Farias, Josivan Regis Oliveira, Aluísio da Silva Silva, Douglas Henrique dos Santos Nascimento, Flávia Raquel F Guerra, Rosane Nassar Meireles Monteiro, Cristina Andrade |
author_sort | da Silva, Anderson França |
collection | PubMed |
description | Vulvovaginal candidiasis is a common fungal infection in women. In this study, Platonia insignis hydroalcoholic extract (PiHE) and its fractions were evaluated for antifungal and antivirulence activities against vaginal Candida species. Dichloromethane (DCMF) and ethyl acetate fractions (EAF) obtained from PiHE effectively inhibited the pathogen. Electrospray ionization mass spectrometry was used for identifying the main compounds in extracts. Minimal inhibitory and fungicidal concentrations (MIC and MFC, respectively) were determined by a broth microdilution assay. Furthermore, we evaluated the effect of the extract and fractions on the virulence properties of Candida albicans, and their cytotoxicity effect was determined on RAW 264.7 cells. Compounds found in extracts were flavonoid glycosides, mainly derivatives of quercetin and myricetin. Extracts showed antifungal potential, with the lowest MIC value for EAF (1.3 mg/mL) and inhibited Candida adherence and biofilm formation. EAF disrupted 48 h biofilms with an inhibition rate of more than 90%. The extract and its fractions exhibited no cytotoxicity. The antifungal effects were attributed to the ability of these extracts to alter the mitochondrial membrane potential for the release of pro-apoptotic factors in the cytosol. In conclusion, our data suggest that PiHE and EAF could act as novel candidates for the development of new therapeutic treatments against fungal infections. |
format | Online Article Text |
id | pubmed-7168675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71686752020-04-20 Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species da Silva, Anderson França da Rocha, Cláudia Quintino da Silva, Luís Cláudio Nascimento Carvalho Júnior, Alexsander Rodrigues Mendes, Iven Neylla Farias Vale de Araruna, Andrea Borges Motta, Elizangela Pestana Silva, Rayssa de Sousa Campos, Carmem Duarte Lima Farias, Josivan Regis Oliveira, Aluísio da Silva Silva, Douglas Henrique dos Santos Nascimento, Flávia Raquel F Guerra, Rosane Nassar Meireles Monteiro, Cristina Andrade Pathogens Article Vulvovaginal candidiasis is a common fungal infection in women. In this study, Platonia insignis hydroalcoholic extract (PiHE) and its fractions were evaluated for antifungal and antivirulence activities against vaginal Candida species. Dichloromethane (DCMF) and ethyl acetate fractions (EAF) obtained from PiHE effectively inhibited the pathogen. Electrospray ionization mass spectrometry was used for identifying the main compounds in extracts. Minimal inhibitory and fungicidal concentrations (MIC and MFC, respectively) were determined by a broth microdilution assay. Furthermore, we evaluated the effect of the extract and fractions on the virulence properties of Candida albicans, and their cytotoxicity effect was determined on RAW 264.7 cells. Compounds found in extracts were flavonoid glycosides, mainly derivatives of quercetin and myricetin. Extracts showed antifungal potential, with the lowest MIC value for EAF (1.3 mg/mL) and inhibited Candida adherence and biofilm formation. EAF disrupted 48 h biofilms with an inhibition rate of more than 90%. The extract and its fractions exhibited no cytotoxicity. The antifungal effects were attributed to the ability of these extracts to alter the mitochondrial membrane potential for the release of pro-apoptotic factors in the cytosol. In conclusion, our data suggest that PiHE and EAF could act as novel candidates for the development of new therapeutic treatments against fungal infections. MDPI 2020-01-28 /pmc/articles/PMC7168675/ /pubmed/32013047 http://dx.doi.org/10.3390/pathogens9020084 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article da Silva, Anderson França da Rocha, Cláudia Quintino da Silva, Luís Cláudio Nascimento Carvalho Júnior, Alexsander Rodrigues Mendes, Iven Neylla Farias Vale de Araruna, Andrea Borges Motta, Elizangela Pestana Silva, Rayssa de Sousa Campos, Carmem Duarte Lima Farias, Josivan Regis Oliveira, Aluísio da Silva Silva, Douglas Henrique dos Santos Nascimento, Flávia Raquel F Guerra, Rosane Nassar Meireles Monteiro, Cristina Andrade Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species |
title | Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species |
title_full | Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species |
title_fullStr | Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species |
title_full_unstemmed | Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species |
title_short | Antifungal and Antivirulence Activities of Hydroalcoholic Extract and Fractions of Platonia insignis Leaves against Vaginal Isolates of Candida Species |
title_sort | antifungal and antivirulence activities of hydroalcoholic extract and fractions of platonia insignis leaves against vaginal isolates of candida species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168675/ https://www.ncbi.nlm.nih.gov/pubmed/32013047 http://dx.doi.org/10.3390/pathogens9020084 |
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