Cargando…

Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos

Newcastle disease (ND) control by vaccination and an institution of biosecurity measures is less feasible in backyard chicken in developing countries. Therefore, an alternative disease control strategy like the genetic selection of less susceptible chicken genotypes is a promising option. In the pre...

Descripción completa

Detalles Bibliográficos
Autores principales: Mpenda, Fulgence Ntangere, Tiambo, Christian Keambou, kyallo, Martina, Juma, John, Pelle, Roger, Lyantagaye, Sylvester Leonard, Buza, Joram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168712/
https://www.ncbi.nlm.nih.gov/pubmed/32328309
http://dx.doi.org/10.1155/2020/5187578
_version_ 1783523701651668992
author Mpenda, Fulgence Ntangere
Tiambo, Christian Keambou
kyallo, Martina
Juma, John
Pelle, Roger
Lyantagaye, Sylvester Leonard
Buza, Joram
author_facet Mpenda, Fulgence Ntangere
Tiambo, Christian Keambou
kyallo, Martina
Juma, John
Pelle, Roger
Lyantagaye, Sylvester Leonard
Buza, Joram
author_sort Mpenda, Fulgence Ntangere
collection PubMed
description Newcastle disease (ND) control by vaccination and an institution of biosecurity measures is less feasible in backyard chicken in developing countries. Therefore, an alternative disease control strategy like the genetic selection of less susceptible chicken genotypes is a promising option. In the present study, genetic polymorphism of LEIO258 marker and association with susceptibility to virulent Newcastle disease virus (NDV) infection in Kuroilers, Sasso, and local Tanzanian chicken embryos were investigated. Samples from high (15%) and less (15%) susceptible cohorts were genotyped by sequencing of LEI0258 marker. A total of 75 DNA sequences comprised of 29 Kuroiler, 29 local Tanzanian chickens, and 17 Sasso were analyzed. Neighbor-joining phylogenetic trees were constructed to depict the clustering of LEI0258 marker alleles and relationship with susceptibility. Alleles with frequency ≥3 were considered for association with susceptibility by the use of the inference technique. The present findings suggest that some LEI0258 marker genetic polymorphisms apart from LEI0258 marker allelic based on sizes may be linked with chicken MHC-B haplotypes that confer chickens variability in resistance or susceptibility to infections. Furthermore, these results demonstrate the presence of relationship between LEI0258 marker polymorphisms and variations in chicken susceptibility to NDV infection, which could be utilized in breeding programs designed to improve chicken disease resistance.
format Online
Article
Text
id pubmed-7168712
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-71687122020-04-23 Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos Mpenda, Fulgence Ntangere Tiambo, Christian Keambou kyallo, Martina Juma, John Pelle, Roger Lyantagaye, Sylvester Leonard Buza, Joram J Pathog Research Article Newcastle disease (ND) control by vaccination and an institution of biosecurity measures is less feasible in backyard chicken in developing countries. Therefore, an alternative disease control strategy like the genetic selection of less susceptible chicken genotypes is a promising option. In the present study, genetic polymorphism of LEIO258 marker and association with susceptibility to virulent Newcastle disease virus (NDV) infection in Kuroilers, Sasso, and local Tanzanian chicken embryos were investigated. Samples from high (15%) and less (15%) susceptible cohorts were genotyped by sequencing of LEI0258 marker. A total of 75 DNA sequences comprised of 29 Kuroiler, 29 local Tanzanian chickens, and 17 Sasso were analyzed. Neighbor-joining phylogenetic trees were constructed to depict the clustering of LEI0258 marker alleles and relationship with susceptibility. Alleles with frequency ≥3 were considered for association with susceptibility by the use of the inference technique. The present findings suggest that some LEI0258 marker genetic polymorphisms apart from LEI0258 marker allelic based on sizes may be linked with chicken MHC-B haplotypes that confer chickens variability in resistance or susceptibility to infections. Furthermore, these results demonstrate the presence of relationship between LEI0258 marker polymorphisms and variations in chicken susceptibility to NDV infection, which could be utilized in breeding programs designed to improve chicken disease resistance. Hindawi 2020-04-08 /pmc/articles/PMC7168712/ /pubmed/32328309 http://dx.doi.org/10.1155/2020/5187578 Text en Copyright © 2020 Fulgence Ntangere Mpenda et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mpenda, Fulgence Ntangere
Tiambo, Christian Keambou
kyallo, Martina
Juma, John
Pelle, Roger
Lyantagaye, Sylvester Leonard
Buza, Joram
Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos
title Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos
title_full Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos
title_fullStr Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos
title_full_unstemmed Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos
title_short Association of LEI0258 Marker Alleles and Susceptibility to Virulent Newcastle Disease Virus Infection in Kuroiler, Sasso, and Local Tanzanian Chicken Embryos
title_sort association of lei0258 marker alleles and susceptibility to virulent newcastle disease virus infection in kuroiler, sasso, and local tanzanian chicken embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168712/
https://www.ncbi.nlm.nih.gov/pubmed/32328309
http://dx.doi.org/10.1155/2020/5187578
work_keys_str_mv AT mpendafulgencentangere associationoflei0258markerallelesandsusceptibilitytovirulentnewcastlediseasevirusinfectioninkuroilersassoandlocaltanzanianchickenembryos
AT tiambochristiankeambou associationoflei0258markerallelesandsusceptibilitytovirulentnewcastlediseasevirusinfectioninkuroilersassoandlocaltanzanianchickenembryos
AT kyallomartina associationoflei0258markerallelesandsusceptibilitytovirulentnewcastlediseasevirusinfectioninkuroilersassoandlocaltanzanianchickenembryos
AT jumajohn associationoflei0258markerallelesandsusceptibilitytovirulentnewcastlediseasevirusinfectioninkuroilersassoandlocaltanzanianchickenembryos
AT pelleroger associationoflei0258markerallelesandsusceptibilitytovirulentnewcastlediseasevirusinfectioninkuroilersassoandlocaltanzanianchickenembryos
AT lyantagayesylvesterleonard associationoflei0258markerallelesandsusceptibilitytovirulentnewcastlediseasevirusinfectioninkuroilersassoandlocaltanzanianchickenembryos
AT buzajoram associationoflei0258markerallelesandsusceptibilitytovirulentnewcastlediseasevirusinfectioninkuroilersassoandlocaltanzanianchickenembryos