Cargando…

Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome

BACKGROUND: In myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), altered membrane excitability often occurs in exercising muscles demonstrating muscle dysfunction regardless of any psychiatric disorder. Increased oxidative stress is also present in many ME/CFS patients and could affect th...

Descripción completa

Detalles Bibliográficos
Autores principales: Jammes, Yves, Adjriou, Nabil, Kipson, Nathalie, Criado, Christine, Charpin, Caroline, Rebaudet, Stanislas, Stavris, Chloé, Guieu, Régis, Fenouillet, Emmanuel, Retornaz, Frédérique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168976/
https://www.ncbi.nlm.nih.gov/pubmed/32306967
http://dx.doi.org/10.1186/s12967-020-02341-9
_version_ 1783523755528552448
author Jammes, Yves
Adjriou, Nabil
Kipson, Nathalie
Criado, Christine
Charpin, Caroline
Rebaudet, Stanislas
Stavris, Chloé
Guieu, Régis
Fenouillet, Emmanuel
Retornaz, Frédérique
author_facet Jammes, Yves
Adjriou, Nabil
Kipson, Nathalie
Criado, Christine
Charpin, Caroline
Rebaudet, Stanislas
Stavris, Chloé
Guieu, Régis
Fenouillet, Emmanuel
Retornaz, Frédérique
author_sort Jammes, Yves
collection PubMed
description BACKGROUND: In myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), altered membrane excitability often occurs in exercising muscles demonstrating muscle dysfunction regardless of any psychiatric disorder. Increased oxidative stress is also present in many ME/CFS patients and could affect the membrane excitability of resting muscles. METHODS: Seventy-two patients were examined at rest, during an incremental cycling exercise and during a 10-min post-exercise recovery period. All patients had at least four criteria leading to a diagnosis of ME/CFS. To explore muscle membrane excitability, M-waves were recorded during exercise (rectus femoris (RF) muscle) and at rest (flexor digitorum longus (FDL) muscle). Two plasma markers of oxidative stress (thiobarbituric acid reactive substance (TBARS) and oxidation–reduction potential (ORP)) were measured. Plasma potassium (K(+)) concentration was also measured at rest and at the end of exercise to explore K(+) outflow. RESULTS: Thirty-nine patients had marked M-wave alterations in both the RF and FDL muscles during and after exercise while the resting values of plasma TBARS and ORP were increased and exercise-induced K(+) outflow was decreased. In contrast, 33 other patients with a diagnosis of ME/CFS had no M-wave alterations and had lower baseline levels of TBARS and ORP. M-wave changes were inversely proportional to TBARS and ORP levels. CONCLUSIONS: Resting muscles of ME/CFS patients have altered muscle membrane excitability. However, our data reveal heterogeneity in some major biomarkers in ME/CFS patients. Measurement of ORP may help to improve the diagnosis of ME/CFS. Trial registration Ethics Committee “Ouest II” of Angers (May 17, 2019) RCB ID: number 2019-A00611-56
format Online
Article
Text
id pubmed-7168976
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-71689762020-04-23 Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome Jammes, Yves Adjriou, Nabil Kipson, Nathalie Criado, Christine Charpin, Caroline Rebaudet, Stanislas Stavris, Chloé Guieu, Régis Fenouillet, Emmanuel Retornaz, Frédérique J Transl Med Research BACKGROUND: In myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), altered membrane excitability often occurs in exercising muscles demonstrating muscle dysfunction regardless of any psychiatric disorder. Increased oxidative stress is also present in many ME/CFS patients and could affect the membrane excitability of resting muscles. METHODS: Seventy-two patients were examined at rest, during an incremental cycling exercise and during a 10-min post-exercise recovery period. All patients had at least four criteria leading to a diagnosis of ME/CFS. To explore muscle membrane excitability, M-waves were recorded during exercise (rectus femoris (RF) muscle) and at rest (flexor digitorum longus (FDL) muscle). Two plasma markers of oxidative stress (thiobarbituric acid reactive substance (TBARS) and oxidation–reduction potential (ORP)) were measured. Plasma potassium (K(+)) concentration was also measured at rest and at the end of exercise to explore K(+) outflow. RESULTS: Thirty-nine patients had marked M-wave alterations in both the RF and FDL muscles during and after exercise while the resting values of plasma TBARS and ORP were increased and exercise-induced K(+) outflow was decreased. In contrast, 33 other patients with a diagnosis of ME/CFS had no M-wave alterations and had lower baseline levels of TBARS and ORP. M-wave changes were inversely proportional to TBARS and ORP levels. CONCLUSIONS: Resting muscles of ME/CFS patients have altered muscle membrane excitability. However, our data reveal heterogeneity in some major biomarkers in ME/CFS patients. Measurement of ORP may help to improve the diagnosis of ME/CFS. Trial registration Ethics Committee “Ouest II” of Angers (May 17, 2019) RCB ID: number 2019-A00611-56 BioMed Central 2020-04-19 /pmc/articles/PMC7168976/ /pubmed/32306967 http://dx.doi.org/10.1186/s12967-020-02341-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Jammes, Yves
Adjriou, Nabil
Kipson, Nathalie
Criado, Christine
Charpin, Caroline
Rebaudet, Stanislas
Stavris, Chloé
Guieu, Régis
Fenouillet, Emmanuel
Retornaz, Frédérique
Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome
title Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome
title_full Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome
title_fullStr Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome
title_full_unstemmed Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome
title_short Altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome
title_sort altered muscle membrane potential and redox status differentiates two subgroups of patients with chronic fatigue syndrome
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168976/
https://www.ncbi.nlm.nih.gov/pubmed/32306967
http://dx.doi.org/10.1186/s12967-020-02341-9
work_keys_str_mv AT jammesyves alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT adjriounabil alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT kipsonnathalie alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT criadochristine alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT charpincaroline alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT rebaudetstanislas alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT stavrischloe alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT guieuregis alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT fenouilletemmanuel alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome
AT retornazfrederique alteredmusclemembranepotentialandredoxstatusdifferentiatestwosubgroupsofpatientswithchronicfatiguesyndrome