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Promising methods for detection of novel coronavirus SARS‐CoV‐2
A very recent outbreak of the novel coronavirus, COVID‐19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169335/ http://dx.doi.org/10.1002/viw2.4 |
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author | Liu, Ran Fu, Aisi Deng, Zixin Li, Yan Liu, Tiangang |
author_facet | Liu, Ran Fu, Aisi Deng, Zixin Li, Yan Liu, Tiangang |
author_sort | Liu, Ran |
collection | PubMed |
description | A very recent outbreak of the novel coronavirus, COVID‐19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, we summarized the six promising methods, including whole‐genome sequencing, real‐time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody‐based immunoassay techniques, use of paper‐based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats‐Cas system‐based technology, which can also be deployed for the detection of SARS‐CoV‐2. We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID‐19. |
format | Online Article Text |
id | pubmed-7169335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71693352020-04-20 Promising methods for detection of novel coronavirus SARS‐CoV‐2 Liu, Ran Fu, Aisi Deng, Zixin Li, Yan Liu, Tiangang View Perspectives A very recent outbreak of the novel coronavirus, COVID‐19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, we summarized the six promising methods, including whole‐genome sequencing, real‐time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody‐based immunoassay techniques, use of paper‐based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats‐Cas system‐based technology, which can also be deployed for the detection of SARS‐CoV‐2. We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID‐19. John Wiley and Sons Inc. 2020-03-23 2020-03 /pmc/articles/PMC7169335/ http://dx.doi.org/10.1002/viw2.4 Text en © 2020 The Authors. VIEW published by John Wiley & Sons Australia, Ltd and Shanghai Fuji Technology Consulting Co., Ltd, authorized by Professional Community of Experimental Medicine, National Association of Health Industry and Enterprise Management (PCEM) This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Perspectives Liu, Ran Fu, Aisi Deng, Zixin Li, Yan Liu, Tiangang Promising methods for detection of novel coronavirus SARS‐CoV‐2 |
title | Promising methods for detection of novel coronavirus SARS‐CoV‐2 |
title_full | Promising methods for detection of novel coronavirus SARS‐CoV‐2 |
title_fullStr | Promising methods for detection of novel coronavirus SARS‐CoV‐2 |
title_full_unstemmed | Promising methods for detection of novel coronavirus SARS‐CoV‐2 |
title_short | Promising methods for detection of novel coronavirus SARS‐CoV‐2 |
title_sort | promising methods for detection of novel coronavirus sars‐cov‐2 |
topic | Perspectives |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169335/ http://dx.doi.org/10.1002/viw2.4 |
work_keys_str_mv | AT liuran promisingmethodsfordetectionofnovelcoronavirussarscov2 AT fuaisi promisingmethodsfordetectionofnovelcoronavirussarscov2 AT dengzixin promisingmethodsfordetectionofnovelcoronavirussarscov2 AT liyan promisingmethodsfordetectionofnovelcoronavirussarscov2 AT liutiangang promisingmethodsfordetectionofnovelcoronavirussarscov2 |