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Promising methods for detection of novel coronavirus SARS‐CoV‐2

A very recent outbreak of the novel coronavirus, COVID‐19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection....

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Autores principales: Liu, Ran, Fu, Aisi, Deng, Zixin, Li, Yan, Liu, Tiangang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169335/
http://dx.doi.org/10.1002/viw2.4
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author Liu, Ran
Fu, Aisi
Deng, Zixin
Li, Yan
Liu, Tiangang
author_facet Liu, Ran
Fu, Aisi
Deng, Zixin
Li, Yan
Liu, Tiangang
author_sort Liu, Ran
collection PubMed
description A very recent outbreak of the novel coronavirus, COVID‐19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, we summarized the six promising methods, including whole‐genome sequencing, real‐time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody‐based immunoassay techniques, use of paper‐based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats‐Cas system‐based technology, which can also be deployed for the detection of SARS‐CoV‐2. We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID‐19.
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spelling pubmed-71693352020-04-20 Promising methods for detection of novel coronavirus SARS‐CoV‐2 Liu, Ran Fu, Aisi Deng, Zixin Li, Yan Liu, Tiangang View Perspectives A very recent outbreak of the novel coronavirus, COVID‐19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, we summarized the six promising methods, including whole‐genome sequencing, real‐time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody‐based immunoassay techniques, use of paper‐based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats‐Cas system‐based technology, which can also be deployed for the detection of SARS‐CoV‐2. We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID‐19. John Wiley and Sons Inc. 2020-03-23 2020-03 /pmc/articles/PMC7169335/ http://dx.doi.org/10.1002/viw2.4 Text en © 2020 The Authors. VIEW published by John Wiley & Sons Australia, Ltd and Shanghai Fuji Technology Consulting Co., Ltd, authorized by Professional Community of Experimental Medicine, National Association of Health Industry and Enterprise Management (PCEM) This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Perspectives
Liu, Ran
Fu, Aisi
Deng, Zixin
Li, Yan
Liu, Tiangang
Promising methods for detection of novel coronavirus SARS‐CoV‐2
title Promising methods for detection of novel coronavirus SARS‐CoV‐2
title_full Promising methods for detection of novel coronavirus SARS‐CoV‐2
title_fullStr Promising methods for detection of novel coronavirus SARS‐CoV‐2
title_full_unstemmed Promising methods for detection of novel coronavirus SARS‐CoV‐2
title_short Promising methods for detection of novel coronavirus SARS‐CoV‐2
title_sort promising methods for detection of novel coronavirus sars‐cov‐2
topic Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169335/
http://dx.doi.org/10.1002/viw2.4
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