Transient Transfection of the Zoonotic Parasite Babesia microti

The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection of B. microti. The plasmids containing the fi...

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Detalles Bibliográficos
Autores principales: Liu, Mingming, Ji, Shengwei, Rizk, Mohamed Abdo, Adjou Moumouni, Paul Franck, Galon, Eloiza May, Li, Jixu, Li, Yongchang, Zheng, Weiqing, Benedicto, Byamukama, Tumwebaze, Maria Agnes, Asada, Masahito, Xuan, Xuenan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169379/
https://www.ncbi.nlm.nih.gov/pubmed/32050586
http://dx.doi.org/10.3390/pathogens9020108
Descripción
Sumario:The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection of B. microti. The plasmids containing the firefly luciferase reporter gene were transfected into B. microti by an AMAXA 4D Nucleofection system. Twenty-four-hour synchronization, the 5′-actin promoter, program FA100, and 50 μg of plasmid DNA constituted the best conditions for the transient transfection of B. microti. This finding is the first step towards a stable transfection method for B. microti, which may contribute to a better understanding of the biology of the parasite.

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