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Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil

Giardiasis is an intestinal infection caused by ingestion of water or food contaminated with cysts of Giardia lamblia. Susceptibility is higher in children and overall prevalence can reach up to 90% in low-income areas, although outbreaks are also reported in developed countries. Both parasite and i...

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Autores principales: Cascais-Figueiredo, Tiara, Austriaco-Teixeira, Phelipe, Fantinatti, Maria, Silva-Freitas, Maria Luciana, Santos-Oliveira, Joanna Reis, Coelho, Camila H., Singer, Steven M., Da-Cruz, Alda Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169386/
https://www.ncbi.nlm.nih.gov/pubmed/31861618
http://dx.doi.org/10.3390/pathogens9010007
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author Cascais-Figueiredo, Tiara
Austriaco-Teixeira, Phelipe
Fantinatti, Maria
Silva-Freitas, Maria Luciana
Santos-Oliveira, Joanna Reis
Coelho, Camila H.
Singer, Steven M.
Da-Cruz, Alda Maria
author_facet Cascais-Figueiredo, Tiara
Austriaco-Teixeira, Phelipe
Fantinatti, Maria
Silva-Freitas, Maria Luciana
Santos-Oliveira, Joanna Reis
Coelho, Camila H.
Singer, Steven M.
Da-Cruz, Alda Maria
author_sort Cascais-Figueiredo, Tiara
collection PubMed
description Giardiasis is an intestinal infection caused by ingestion of water or food contaminated with cysts of Giardia lamblia. Susceptibility is higher in children and overall prevalence can reach up to 90% in low-income areas, although outbreaks are also reported in developed countries. Both parasite and immune-mediated epithelial damage has been observed in vitro and in animal models. However, whether enterocytes are directly damaged during infection is not entirely known. Our goal was to identify whether plasma levels of intestinal fatty acid binding protein (I-FABP), a marker of enterocyte damage, are related to the immune response in giardiasis. Blood plasma was collected from 31 children (19 Giardia-positive) from a public day care in Rio de Janeiro, Brazil. The levels of I-FABP were increased in Giardia-infected children compared to children without detectable infection. There was no difference in I-FABP levels in giardiasis caused by different genetic assemblages of Giardia. Levels of IL-8 were decreased, while there was a trend to elevated IL-17 in the Giardia-positive children. A positive correlation was observed between I-FABP and IL-17 levels as well as TNF, suggesting that epithelial damage can be related to cytokine production during giardiasis. These results help elucidate the relationship between the disruption of the intestinal mucosal barrier and immune responses to G. lamblia in children.
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spelling pubmed-71693862020-04-20 Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil Cascais-Figueiredo, Tiara Austriaco-Teixeira, Phelipe Fantinatti, Maria Silva-Freitas, Maria Luciana Santos-Oliveira, Joanna Reis Coelho, Camila H. Singer, Steven M. Da-Cruz, Alda Maria Pathogens Article Giardiasis is an intestinal infection caused by ingestion of water or food contaminated with cysts of Giardia lamblia. Susceptibility is higher in children and overall prevalence can reach up to 90% in low-income areas, although outbreaks are also reported in developed countries. Both parasite and immune-mediated epithelial damage has been observed in vitro and in animal models. However, whether enterocytes are directly damaged during infection is not entirely known. Our goal was to identify whether plasma levels of intestinal fatty acid binding protein (I-FABP), a marker of enterocyte damage, are related to the immune response in giardiasis. Blood plasma was collected from 31 children (19 Giardia-positive) from a public day care in Rio de Janeiro, Brazil. The levels of I-FABP were increased in Giardia-infected children compared to children without detectable infection. There was no difference in I-FABP levels in giardiasis caused by different genetic assemblages of Giardia. Levels of IL-8 were decreased, while there was a trend to elevated IL-17 in the Giardia-positive children. A positive correlation was observed between I-FABP and IL-17 levels as well as TNF, suggesting that epithelial damage can be related to cytokine production during giardiasis. These results help elucidate the relationship between the disruption of the intestinal mucosal barrier and immune responses to G. lamblia in children. MDPI 2019-12-19 /pmc/articles/PMC7169386/ /pubmed/31861618 http://dx.doi.org/10.3390/pathogens9010007 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cascais-Figueiredo, Tiara
Austriaco-Teixeira, Phelipe
Fantinatti, Maria
Silva-Freitas, Maria Luciana
Santos-Oliveira, Joanna Reis
Coelho, Camila H.
Singer, Steven M.
Da-Cruz, Alda Maria
Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil
title Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil
title_full Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil
title_fullStr Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil
title_full_unstemmed Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil
title_short Giardiasis Alters Intestinal Fatty Acid Binding Protein (I-FABP) and Plasma Cytokines Levels in Children in Brazil
title_sort giardiasis alters intestinal fatty acid binding protein (i-fabp) and plasma cytokines levels in children in brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169386/
https://www.ncbi.nlm.nih.gov/pubmed/31861618
http://dx.doi.org/10.3390/pathogens9010007
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