Cargando…

Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence

Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is assoc...

Descripción completa

Detalles Bibliográficos
Autores principales: Silva, Karla Christina Sousa, Silva, Lana O’Hara Souza, Silva, Guilherme Algusto Alves, Borges, Clayton Luiz, Novaes, Evandro, Paccez, Juliano Domiraci, Fontes, Wagner, Giambiagi-deMarval, Marcia, Soares, Célia Maria de Almeida, Parente-Rocha, Juliana Alves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169411/
https://www.ncbi.nlm.nih.gov/pubmed/31963821
http://dx.doi.org/10.3390/pathogens9010069
_version_ 1783523784253243392
author Silva, Karla Christina Sousa
Silva, Lana O’Hara Souza
Silva, Guilherme Algusto Alves
Borges, Clayton Luiz
Novaes, Evandro
Paccez, Juliano Domiraci
Fontes, Wagner
Giambiagi-deMarval, Marcia
Soares, Célia Maria de Almeida
Parente-Rocha, Juliana Alves
author_facet Silva, Karla Christina Sousa
Silva, Lana O’Hara Souza
Silva, Guilherme Algusto Alves
Borges, Clayton Luiz
Novaes, Evandro
Paccez, Juliano Domiraci
Fontes, Wagner
Giambiagi-deMarval, Marcia
Soares, Célia Maria de Almeida
Parente-Rocha, Juliana Alves
author_sort Silva, Karla Christina Sousa
collection PubMed
description Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive host immune defenses. In this work, we presented the comparative proteomic profile of three S. saprophyticus strains. It was possible to characterize differences in the proteome content, specially related to expression of virulence factors. We compiled this data and previous data and we detected one strain (9325) possessing higher production and secretion of proteins related to virulence. Our results show that phenotypic, genotypic, and proteomic differences reflect in the ability to survive during interaction with host cells, since the 9325 strain presented a higher survival rate after macrophage interaction. In counterpart, the 7108 strain that possesses lower content of proteins related to virulence presented higher ability to form biofilm suggesting that this strain can be better adapted to persist in the host and in the environment. Our work describes, for the first time, proteomic flexibility among S. saprophyticus strains, reflecting in virulence and persistence.
format Online
Article
Text
id pubmed-7169411
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71694112020-04-20 Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence Silva, Karla Christina Sousa Silva, Lana O’Hara Souza Silva, Guilherme Algusto Alves Borges, Clayton Luiz Novaes, Evandro Paccez, Juliano Domiraci Fontes, Wagner Giambiagi-deMarval, Marcia Soares, Célia Maria de Almeida Parente-Rocha, Juliana Alves Pathogens Article Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive host immune defenses. In this work, we presented the comparative proteomic profile of three S. saprophyticus strains. It was possible to characterize differences in the proteome content, specially related to expression of virulence factors. We compiled this data and previous data and we detected one strain (9325) possessing higher production and secretion of proteins related to virulence. Our results show that phenotypic, genotypic, and proteomic differences reflect in the ability to survive during interaction with host cells, since the 9325 strain presented a higher survival rate after macrophage interaction. In counterpart, the 7108 strain that possesses lower content of proteins related to virulence presented higher ability to form biofilm suggesting that this strain can be better adapted to persist in the host and in the environment. Our work describes, for the first time, proteomic flexibility among S. saprophyticus strains, reflecting in virulence and persistence. MDPI 2020-01-19 /pmc/articles/PMC7169411/ /pubmed/31963821 http://dx.doi.org/10.3390/pathogens9010069 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Karla Christina Sousa
Silva, Lana O’Hara Souza
Silva, Guilherme Algusto Alves
Borges, Clayton Luiz
Novaes, Evandro
Paccez, Juliano Domiraci
Fontes, Wagner
Giambiagi-deMarval, Marcia
Soares, Célia Maria de Almeida
Parente-Rocha, Juliana Alves
Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
title Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
title_full Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
title_fullStr Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
title_full_unstemmed Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
title_short Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
title_sort staphylococcus saprophyticus proteomic analyses elucidate differences in the protein repertories among clinical strains related to virulence and persistence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169411/
https://www.ncbi.nlm.nih.gov/pubmed/31963821
http://dx.doi.org/10.3390/pathogens9010069
work_keys_str_mv AT silvakarlachristinasousa staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT silvalanaoharasouza staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT silvaguilhermealgustoalves staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT borgesclaytonluiz staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT novaesevandro staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT paccezjulianodomiraci staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT fonteswagner staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT giambiagidemarvalmarcia staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT soaresceliamariadealmeida staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence
AT parenterochajulianaalves staphylococcussaprophyticusproteomicanalyseselucidatedifferencesintheproteinrepertoriesamongclinicalstrainsrelatedtovirulenceandpersistence