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Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is assoc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169411/ https://www.ncbi.nlm.nih.gov/pubmed/31963821 http://dx.doi.org/10.3390/pathogens9010069 |
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author | Silva, Karla Christina Sousa Silva, Lana O’Hara Souza Silva, Guilherme Algusto Alves Borges, Clayton Luiz Novaes, Evandro Paccez, Juliano Domiraci Fontes, Wagner Giambiagi-deMarval, Marcia Soares, Célia Maria de Almeida Parente-Rocha, Juliana Alves |
author_facet | Silva, Karla Christina Sousa Silva, Lana O’Hara Souza Silva, Guilherme Algusto Alves Borges, Clayton Luiz Novaes, Evandro Paccez, Juliano Domiraci Fontes, Wagner Giambiagi-deMarval, Marcia Soares, Célia Maria de Almeida Parente-Rocha, Juliana Alves |
author_sort | Silva, Karla Christina Sousa |
collection | PubMed |
description | Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive host immune defenses. In this work, we presented the comparative proteomic profile of three S. saprophyticus strains. It was possible to characterize differences in the proteome content, specially related to expression of virulence factors. We compiled this data and previous data and we detected one strain (9325) possessing higher production and secretion of proteins related to virulence. Our results show that phenotypic, genotypic, and proteomic differences reflect in the ability to survive during interaction with host cells, since the 9325 strain presented a higher survival rate after macrophage interaction. In counterpart, the 7108 strain that possesses lower content of proteins related to virulence presented higher ability to form biofilm suggesting that this strain can be better adapted to persist in the host and in the environment. Our work describes, for the first time, proteomic flexibility among S. saprophyticus strains, reflecting in virulence and persistence. |
format | Online Article Text |
id | pubmed-7169411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71694112020-04-20 Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence Silva, Karla Christina Sousa Silva, Lana O’Hara Souza Silva, Guilherme Algusto Alves Borges, Clayton Luiz Novaes, Evandro Paccez, Juliano Domiraci Fontes, Wagner Giambiagi-deMarval, Marcia Soares, Célia Maria de Almeida Parente-Rocha, Juliana Alves Pathogens Article Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive host immune defenses. In this work, we presented the comparative proteomic profile of three S. saprophyticus strains. It was possible to characterize differences in the proteome content, specially related to expression of virulence factors. We compiled this data and previous data and we detected one strain (9325) possessing higher production and secretion of proteins related to virulence. Our results show that phenotypic, genotypic, and proteomic differences reflect in the ability to survive during interaction with host cells, since the 9325 strain presented a higher survival rate after macrophage interaction. In counterpart, the 7108 strain that possesses lower content of proteins related to virulence presented higher ability to form biofilm suggesting that this strain can be better adapted to persist in the host and in the environment. Our work describes, for the first time, proteomic flexibility among S. saprophyticus strains, reflecting in virulence and persistence. MDPI 2020-01-19 /pmc/articles/PMC7169411/ /pubmed/31963821 http://dx.doi.org/10.3390/pathogens9010069 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Silva, Karla Christina Sousa Silva, Lana O’Hara Souza Silva, Guilherme Algusto Alves Borges, Clayton Luiz Novaes, Evandro Paccez, Juliano Domiraci Fontes, Wagner Giambiagi-deMarval, Marcia Soares, Célia Maria de Almeida Parente-Rocha, Juliana Alves Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence |
title | Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence |
title_full | Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence |
title_fullStr | Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence |
title_full_unstemmed | Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence |
title_short | Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence |
title_sort | staphylococcus saprophyticus proteomic analyses elucidate differences in the protein repertories among clinical strains related to virulence and persistence |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169411/ https://www.ncbi.nlm.nih.gov/pubmed/31963821 http://dx.doi.org/10.3390/pathogens9010069 |
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